饲料中的玉米蛋白质对军曹鱼幼鱼蛋白质生长的贡献率

周晖; 陈刚; 古滨河; 董晓慧

doi:10.3969/j.issn.1006-267x.2013.11.016

动物营养学报 >

2013 , Vol. 25 >Issue 11: 2633 - 2642

DOI: https://doi.org/10.3969/j.issn.1006-267x.2013.11.016

水产营养 Aquaculture Nutrition

饲料中的玉米蛋白质对军曹鱼幼鱼蛋白质生长的贡献率

周晖 ,
陈刚 ,
古滨河 ,
董晓慧

展开

1. 广东海洋大学水产学院, 湛江 524025;
2. 广东省水产经济动物病原生物学及流行病学重点实验室, 湛江 524025;
3. 广东省普通高校南海水产经济动物增养殖重点实验室, 湛江 524025

收稿日期: 2013-05-22

网络出版日期: 2013-10-27

基金资助

国家海洋局海洋公益性行业科研专项项目(201205028);广东省海洋经济创新发展区域示范专项经费(GD2012-A01-007);广东省科技计划项目(2009B020308005,用2010B080203044);广东省海洋与渔业科技项目(A2009-08D03,A2010-08D04);广东海洋大学2008年校选项目"军曹鱼幼鱼碳、氮稳定同位素分馏实验(0812068)"

收起

Contribution Rate of Dietary Corn Protein to Protein Growth of Juvenile Cobia (Rachycentron canadum L.)

ZHOU Hui ,
CHEN Gang ,
GU Binhe ,
DONG Xiaohui

Expand

1. College of Fishery, Guangdong Ocean University, Zhanjiang 524025, China;
2. Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Animals, Zhanjiang 524025, China;
3. Guangdong South China Sea Key Laboratory of Aquaculture for Aquatic Animals, Zhanjiang 524025, China

Received date: 2013-05-22

Online published: 2013-10-27

Fold

摘要

本试验旨在研究饲料中的玉米蛋白质对军曹鱼幼鱼蛋白质生长的贡献率,并估算军曹鱼幼鱼的碳、氮元素周转时间。试验配制2种等氮等能的饲料,对照饲料以鱼粉为蛋白质源,试验饲料在对照饲料的基础上以玉米蛋白质(玉米蛋白粉形式)替代10%鱼粉蛋白质,分别投喂初始体质量为(21.34±1.54) g的军曹鱼幼鱼24 d后采样,进行碳、氮稳定同位素值测定和数学模型分析。对照组和试验组各设3个重复,每个重复40尾鱼。结果表明:试验组幼鱼的摄食量与对照组无显著差异(P>0.05),而其增重率、饲料转化率、蛋白质效率和特定生长率则均显著小于对照组(P<0.05)。试验组幼鱼全鱼碳、氮元素周转半时(以T₅₀表示)(5.72和9.88 d)与对照组(6.03和9.55 d)均无显著差异(P>0.05);而其肌肉碳、氮元素T₅₀(7.79和10.01 d)与对照组(6.99和9.37 d)均有显著差异(P<0.05)。玉米蛋白质在军曹鱼幼鱼全鱼和肌肉蛋白质生长中的贡献率分别为7.64%和9.62%,存在显著差异(P<0.05)。结果提示,玉米蛋白质对军曹鱼幼鱼蛋白质生长的实际贡献率小于其在饲料蛋白质中所占比例,替代10%鱼粉蛋白质后降低了军曹鱼幼鱼的生长性能和碳、氮元素周转速度,如以其替代军曹鱼幼鱼饲料中的鱼粉(不补充必需氨基酸),替代比例应小于10%。

关键词： 军曹鱼幼鱼; 碳稳定同位素值; 氮稳定同位素值; 玉米蛋白质; 蛋白质生长; 贡献率

本文引用格式

周晖 , 陈刚 , 古滨河 , 董晓慧 . 饲料中的玉米蛋白质对军曹鱼幼鱼蛋白质生长的贡献率[J]. 动物营养学报, 2013 , 25(11) : 2633 -2642 . DOI: 10.3969/j.issn.1006-267x.2013.11.016

Abstract

The purpose of this experiment was to investigate the contribution rate of dietary corn protein to protein growth of juvenile cobia (Rachycentron canadum L.), and estimate the turnover time of carbon and nitrogen elements in juvenile cobia. Two isonitrogenous and isocaloric diets were formulated: the control group diet was prepared with fish meal as a protein source, and experimental diet was prepared with corn protein (corn gluten meal form) to replace 10% fish meal on the basis of the control group diet. After 24 days feeding for juvenile cobia with an average body weight of (21.34±1.54) g, the carbon and nitrogen stable isotope values of sampled fishes were measured and assessed by mathematical model. There were 3 replicates in control group and experimental group, respectively, and each replicate had 40 fish. The results showed that there was no significant difference in feed intake between the experimental group and control group (P>0.05), but the weight gain rate, feed conversion ratio, protein efficiency ratio and special growth rate of fish fed experimental diet were significantly lower than those of fish fed control diet (P<0.05). There was no significant difference between the turnover half life (represented by T₅₀) of carbon and nitrogen for experiment group (5.72 and 9.88 days, respectively) and control group (6.03 and 9.55 days, respectively) in whole fish (P>0.05), and there was a significant difference between T₅₀ of carbon and nitrogen for experimental group (7.79 and 10.01 days, respectively) and control group (6.99 and 9.37 days, respectively) in muscle (P<0.05). The contribution rate of corn protein to the protein growth of juvenile cobia whole fish and muscle was 7.64% and 9.62%, respectively, and there was a significant difference between them (P<0.05). It is concluded that the actual contribution rate of dietary corn protein to the protein growth of juvenile cobia is lower than its percentage in the dietary protein; when 10% fish meal in the diet is replaced by corn protein, the growth performance and turnover rate of carbon and nitrogen elements are reduced. The corn protein should not replace more than 10% fish meal in the diet when it is used as an substitute for fish meal (without essential amino acid supplementation).

Key words： juvenile cobia; δ¹³C; δ¹⁵N; corn protein; protein growth; contribution rate

参考文献

[1] CHOU R L,SU M S,CHEN H Y.Optimal dietary protein and lipid levels for juvenile cobia(Rachycentron canadum)[J].Aquaculture,2001,193(1/2):81-89.

[2] 周歧存,麦康森,刘永坚,等.动植物蛋白源替代鱼粉研究进展[J].水产学报,2005,29(3):404-410.

[3] 周晖,陈刚,林小涛.三种蛋白源部分替代鱼粉对军曹鱼幼鱼生长和体成分的影响[J].水产科学,2012,31(6):311-315.

[4] GAMBOA-DELGADO J.Application of natural stable isotopes in aquaculture nutrition[D].Ph.D.Thesis.Bangor:University of Wales,2009:27.

[5] PHILLIPS D L,GREGG J W.Uncertainty in source partitioning using stable isotopes[J].Oecologia,2001,127(2):171-179.

[6] HESSLEIN R H,HALLARD K A,RAMLAL P.Replacement of sulfur,carbon,and nitrogen in tissue of growing broad whitefish (Coregonus nasus) in response to a change in diet traced by ³⁴S,¹³C,and ¹⁵N[J].Canadian Journal of Fisheries and Aquatic Sciences,1993,50(10):2071-2076.

[7] 王广军,吴锐全,谢骏,等.军曹鱼饲料中用豆粕代替鱼粉的研究[J].大连水产学院学报,2005,20(4):304-307.

[8] ZHOU Q C,MAI K S,TAN B P,et al.Partial replacement of fishmeal by soybean meal in diets for juvenile cobia (Rachycentron canadum)[J].Aquaculture Nutrition,2005,11(3):175-182.

[9] ANGELA N L,CRAIG S R,MCLEAN E.Replacement of fish meal in cobia (Rachycentron canadum) diets using an organically certified protein[J].Aquaculture,2006,257(1/2/3/4):393-399.

[10] ANGELA N L,MCLEAN E,CRAIG S R.The effects of organic protein supplementation upon growth,feed conversion and texture quality parameters of juvenile cobia (Rachycentron canadum)[J].Aquaculture,2007,264(1/2/3/4):342-352.

[11] 晏家友,贾刚.玉米蛋白粉的营养价值及其应用概况[J].饲料工业,2009,30(15):53-55.

[12] ZHOU Q C,TAN B P,MAI K S,et al.Apparent digestibility of selected feed ingredients for juvenile cobia Rachycentron canadum[J].Aquaculture,2004,241(1/2/3/4):441-451.

[13] TIBBETTS S M,MILLEY J E,LALL S P.Apparent protein and energy digestibility of common and alternative feed ingredients by Atlantic cod,Gadus morhua (Linnaeus,1758)[J].Aquaculture,2006,261(4):1314-1327.

[14] ROBAINA L,MOYANO F J,IZQUIERDO M S,et al.Corn gluten and meat and bone meals as protein sources in diets for gilthead sea bream (Sparus aurata):nutritional and histological implications[J].Aquaculture,1997,157(3/4):347-359.

[15] 钟国防,韩斌,华雪铭,等.暗纹东方鲀对几种蛋白质原料表观消化率及酶制剂对其消化能力的影响[J].上海海洋大学学报,2012,21(2):241-246.

[16] 明建华,叶金云,张易祥,等.青鱼对8种饲料原料中营养物质的表观消化率[J].动物营养学报,2012,24(10):2050-2058.

[17] 梁丹妮,姜雪姣,刘文斌,等.建鲤对6种非常规蛋白质原料中营养物质的表观消化率[J].动物营养学报,2011,23(6):1065-1072.

[18] 姜雪姣,梁丹妮,刘文斌,等.团头鲂对8种非常规饲料原料中营养物质的表观消化率[J].水产学报,2011,35(6):932-939.

[19] PEREIRA T G,OLIVA-TELES A.Evaluation of corn gluten meal as a protein source in diets for gilthead sea bream (Sparus aurata L.) juveniles[J].Aquaculture Research,2003,34(13):1111-1117.

[20] HEIDI A L,CHRISTOPHER C K.Corn gluten meal partially replaces dietary fish meal without compromising growth or fatty acid composition of sunshine bass[J].North American Journal of Aquaculture,2008,70(1):50-60.

[21] KOTARO K.Partial replacement of fish meal with corn gluten meal in diets for Japanese flounder Paralichthys olivaceus[J].Journal of the World Aquaculture Society,1999,30(3):357-363.

[22] REGOST C,ARZEL J,KAUSHIK S J.Partial or total replacement of fish meal by corn gluten meal in diet for turbot (Psetta maxima)[J].Aquaculture,1999,180(1/2):99-117.

[23] 刘兴旺,麦康森,艾庆辉,等.玉米蛋白粉替代鱼粉对大菱鲆摄食、生长及体组成的影响[J].水产学报,2012,36(3):466-472.

[24] 陈然,华雪铭,黄旭雄,等.玉米蛋白粉替代鱼粉对异育银鲫生长、蛋白酶活性及表观消化率的影响[J].上海交通大学学报:农业科学版,2009,27(4):358-362,367.

[25] 林峰,蔡木易,易维学.玉米蛋白深加工现状及发展趋势[J].食品与发酵工业,2006,32(11):123-127.

[26] DIAS J,ALVAREZ M J,ARZEL J,et al.Dietary protein source affects lipid metabolism in the European seabass (Dicentrarchus labrax)[J].Comparative Biochemistry and Physiology Part A:Molecular and Integrative Physiology,2005,142(1):19-31.

[27] 王军,华雪铭,邢思华,等.玉米蛋白粉部分替代鱼粉对网箱养殖异育银鲫肌肉组织氮磷保留率的影响[J].上海海洋大学学报,2011,20(5):741-747.

[28] GAMBOA-DELGADO J,CAÑAVATE J P,ZEROLO R,et al.Natural carbon stable isotope ratios as indicators of the relative contribution of live and inert diets to growth in larval Senegalese sole (Solea senegalensis)[J].Aquaculture,2008,280(1):190-197.

[29] GAMBOA-DELGADO J,LE VAY L.Natural stable isotopes as indicators of the relative contribution of soy protein and fish meal to tissue growth in Pacific white shrimp (Litopenaeus vannamei) fed compound diets[J].Aquaculture,2009,291(1/2):115-123.

[30] BELTRÁN M,FERNÁNDEZ-BORRÁS J,MÉDALE F,et al.Natural abundance of ¹⁵N and ¹³C in fish tissues and the use of stable isotopes as dietary protein tracers in rainbow trout and gilthead sea bream[J].Aquaculture Nutrition,2009,15(1):9-18.

Options

文章导航

摘要

本文引用格式

Abstract

参考文献

关于本刊

在线期刊

作者中心

相关单位

联系我们

模态框（Modal）标题

摘要

本文引用格式

Abstract

参考文献

关于本刊

在线期刊

作者中心

相关单位

联系我们