动物营养学报 >

2013 , Vol. 25 >Issue 11: 2763 - 2769

DOI: https://doi.org/10.3969/j.issn.1006-267x.2013.11.033

研究简报 Short Communications

干酒糟及其可溶物和豆粕混合饲料蛋白质二级结构与瘤胃降解特性的关系

  • 吴鹏华 ,
  • 刘大森 ,
  • 仝泳 ,
  • 徐杨 ,
  • 郑立鑫
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  • 1. 东北农业大学动物科技学院, 哈尔滨 150030;
    2. 东北农业大学理学院, 哈尔滨 150030;
    3. 东北农业大学生命科学学院, 哈尔滨 150030

收稿日期: 2013-05-11

  网络出版日期: 2013-10-27

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Relationship between Protein Secondary Structure and Rumen Degradation Characteristics of Mixed Feeds of Distillers Dried Grains with Solubles Combined with Soybean Meal

  • WU Penghua ,
  • LIU Dasen ,
  • TONG Yong ,
  • XU Yang ,
  • ZHENG Lixin
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  • 1. College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China;
    2. College of AnimalScience, Northeast Agricultural University, Harbin 150030, China;
    3. College of Science, Northeast Agricultural University, Harbin 150030, China

Received date: 2013-05-11

  Online published: 2013-10-27

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摘要

本研究旨在探讨干酒糟及其可溶物(DDGS)和豆粕混合饲料蛋白质二级结构与瘤胃降解特性的关系。选用3头安装永久性瘤胃瘘管的荷斯坦奶牛为试验动物,将DDGS、豆粕按照不同比例(DDGS:豆粕分别为100:0、70:30、50:50、30:70、0:100)混合,作为混合饲料样品。通过尼龙袋法测定样品的粗蛋白质瘤胃降解参数及有效降解率,采用傅里叶变换红外光谱技术测定样品的蛋白质二级结构。结果表明:混合饲料样品的蛋白质二级结构中α-螺旋/β-折叠与饲料粗蛋白质有效降解率具有显著的正相关关系(r=0.976 5,P=0.004 3)。可见,可以通过测定蛋白质二级结构来预测混合饲料粗蛋白质有效降解率,蛋白质α-螺旋/β-折叠越高,饲料粗蛋白质有效降解率越高。

关键词： 奶牛; 傅里叶变换红外光谱; 蛋白质二级结构; 有效降解率

本文引用格式

吴鹏华 , 刘大森 , 仝泳 , 徐杨 , 郑立鑫 . 干酒糟及其可溶物和豆粕混合饲料蛋白质二级结构与瘤胃降解特性的关系[J]. 动物营养学报, 2013 , 25(11) : 2763 -2769 . DOI: 10.3969/j.issn.1006-267x.2013.11.033

Abstract

The objective of this study was to investigate the relationship between protein secondary structure and rumen degradation characteristics of mixed feeds of distillers dried grains with solubles (DDGS) combined with soybean meal. The DDGS and soybean meal were mixed at five different ratios (100:0, 75:25, 50:50, 25:75 and 0:100) to make five mixed feed samples. Three Holstein cows fitted with permanent rumen fistulas were used to estimate rumen degradation parameters and efficient degradation rate of crude protein by nylon bags method, and to determine protein secondary structure by Fourier transform infrared spectroscopy (FTIR) technique. The results showed that the α-helix to β-sheet ratio of protein structure of mixed feed samples had a strongly positive correlation with efficient degradation rate of crude protein (r=0.976 5, P=0.004 3). The results indicate that the efficient degradation rate of crude protein can be predicted by protein secondary structure. A higher α-helix to β-sheet ratio results in a higher efficient degradation rate in mixed feeds.

Key words： dairy cows; FTIR; protein secondary structure; efficient degradation rate

参考文献

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