本试验旨在研究蒲公英粗多糖对断奶至3月龄生长肉兔生长性能及免疫功能的影响,为开发应用新型饲料添加剂提供依据。选择160只平均体重为(650±50) g的35日龄的断奶仔兔,随机分为4组,每组2个重复,每个重复20只兔。对照组(Ⅰ组)饲喂基础饲粮,试验组(Ⅱ、Ⅲ、Ⅳ组)分别在基础饲粮中添加0.1%、0.3%、0.5%的蒲公英粗多糖。试验期为50 d。结果表明:1)Ⅲ、Ⅳ组分别平均日增重显著(P<0.05)和极显著(P<0.01)高于对照组,Ⅳ组料重比极显著低于对照组和Ⅱ组(P<0.01)。2)Ⅱ、Ⅲ、Ⅳ组胸腺指数极显著高于对照组(P<0.01),Ⅲ、Ⅳ组脾脏指数极显著高于对照组(P<0.01)。3)各组血清免疫球蛋白A含量无显著差异(P>0.05);Ⅲ、Ⅳ组血清免疫球蛋白M含量极显著高于对照组(P<0.01),免疫球蛋白G含量显著高于对照组(P<0.05)。由此可见,饲粮中添加蒲公英粗多糖可提高断奶至3月龄生长肉兔的生长性能和免疫功能,适宜添加量为0.5%。
This experiment was conducted to study the effects of Taraxacum polysaccharides on growth performance and immune function of growing meat rabbits from weaner to 3 months of age, and to provide a theoretical support for exploitation of a new-style feed additive. A total of 160 35-day-old weaner rabbits with average body weight of (650±50) g were randomly allocated into 4 groups with 2 replicates per group and 20 rabbits per replicate. The control group (group Ⅰ) was fed a basal diet and experimental groups (groups Ⅱ, Ⅲ and Ⅳ) were fed the basal diets supplemented with 0.1%, 0.3% and 0.5% Taraxacum polysaccharides, respectively. The experiment lasted for 50 days. The results showed as follows: 1) the average daily gain in groups Ⅲ and Ⅳ was significantly higher than that in control group (P<0.05 and P<0.01,respectively), the ratio of feed to gain in group Ⅳ was significantly higher than that in control group and group Ⅱ (P<0.01). 2) The spleen index in groups Ⅱ, Ⅲ and Ⅳ was significantly higher than that in control group (P<0.01), and the thymus index in groups Ⅲ and Ⅳ was significantly higher than that in control group (P<0.01). 3) There was no significant difference in serum immunoglobulin A content among all groups (P>0.05), and the serum immunoglobulin M content in groups Ⅲ and Ⅳ was significantly higher than that in control group (P<0.01), the serum immunoglobulin G content in groups Ⅲ and Ⅳ was significantly higher than that in control group (P<0.05). It is concluded that dietary Taraxacum polysaccharides can improve growth performance and immune function of growing meat rabbits from weaner to 3 months of age,the appropriate supplementation is 0.5%.
[1] 周荣汉.药用植物化学分类学[M].上海:上海科技出版社,1989:55.
[2] 文贵辉,李丽立,张彬,等.白术粗多糖对樱桃谷鸭生长性能的影响[J].农业现代化研究,2008,29(3):379-381.
[3] 李红梅,吴山,夏春辉.药食佳品蒲公英的医疗营养保健功能及应用研究与开发利用[J].齐齐哈尔医学院学报,2006,27(6):673-674.
[4] 杨秀女,孟艳,郑丽丽,等.黄芪多糖粉对兔瘟疫苗免疫效果及增重的影响[J].养殖与饲料,2011(3):3-4.
[5] 赵庆友.泰山槐花多糖的提取及对鸡兔免疫增强作用的研究[D].硕士学位论文.泰安:山东农业大学,2012:32-34.
[6] 王世若,王兴龙,韩文瑜.现代动物免疫学[M].长春:吉林科学技术出版社,2001:373-382.
[7] 陈福星,陈文英,郝艳霜.蒲公英多糖对小鼠免疫器官的影响[J].动物医学进展,2008,29(4):10-12.
[8] 刘永杰,李庆章,郝艳红.黄芪多糖和香菇多糖对雏鸡IL-2活性和淋巴细胞增殖反应的影响[J].吉林农业大学学报,1999,21(3):91-98.
[9] 王俊丽,章世元,徐春燕,等.黄芪多糖对肉仔鸡生产性能和部分免疫指标的影响[J].中国饲料,2010(7):18-21.
[10] 梁英,姜宁,何雯娟,等.黄芩多糖对肉仔鸡生长性能和免疫功能的影响[J].动物营养学报,2010,22(4):1031-1036.
[11] 周锐丽,卢峰,秦龙龙.蒲公英的营养与保健功能[J].中国食物与营养,2011,17(6):71-72.