维生素D3对丝毛乌鸡组织白细胞介素2和白细胞介素18基因相对表达量的影响

李思明; 唐艳强; 谷德平; 肖海红; 韦启鹏; 欧阳玲花

doi:10.3969/j.issn.1006-267x.2014.01.026

动物营养学报 >

2014 , Vol. 26 >Issue 1: 197 - 202

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.01.026

饲料营养 Feed Science and Technology

维生素D₃对丝毛乌鸡组织白细胞介素2和白细胞介素18基因相对表达量的影响

李思明 ,
唐艳强 ,
谷德平 ,
肖海红 ,
韦启鹏 ,
欧阳玲花

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江西省农业科学院畜牧兽医研究所, 南昌 330200

收稿日期: 2013-07-22

网络出版日期: 2013-12-24

基金资助

国家自然科学基金项目资助（31160500）；江西省青年科学家培养计划项目资助（20112BCB23030）

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Effects of Vitamin D₃ on the Relative Expression Levels of Interleukin 2 and Interleukin 18 Genes in Tissues of Silky Fowls

LI Siming ,
TANG Yanqiang ,
GU Deping ,
XIAO Haihong ,
WEI Qipeng ,
OUYANG Linghua

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Institute of Animal Husbandry and Veterinary, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China

Received date: 2013-07-22

Online published: 2013-12-24

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摘要

本试验旨在研究维生素D₃对丝毛乌鸡组织白细胞介素2（IL-2）和白细胞介素18（IL-18）基因相对表达量的影响。试验选用120只丝毛乌鸡，随机分为5个处理，每个处理4个重复，每个重复6只鸡。对照处理饲喂基础饲粮，各试验处理分别饲喂在基础饲粮中添加800、1 600、3 200和6 400 IU/kg维生素D₃的试验饲粮。常规笼养6周。采集胸腺、十二指肠、空肠、回肠、盲肠和法氏囊，检测IL-2和IL-18基因相对表达量。结果表明：6 400 IU/kg的维生素D₃上调了IL-2基因在十二指肠、法氏囊和盲肠组织中的相对表达量，而未见其在胸腺、空肠和回肠组织中的相对表达量上调；6 400 IU/kg的维生素D₃上调了IL-18基因在丝毛乌鸡胸腺、回肠、盲肠和法氏囊组织中的相对表达量。结果提示，饲粮维生素D₃添加水平与丝毛乌鸡体内IL-2和IL-18基因相对表达量相关，但作用存在组织差异。

关键词： 丝毛乌鸡; 维生素D₃; 白细胞介素

本文引用格式

李思明 , 唐艳强 , 谷德平 , 肖海红 , 韦启鹏 , 欧阳玲花 . 维生素D₃对丝毛乌鸡组织白细胞介素2和白细胞介素18基因相对表达量的影响[J]. 动物营养学报, 2014 , 26(1) : 197 -202 . DOI: 10.3969/j.issn.1006-267x.2014.01.026

Abstract

The aim of this experiment was to research the effects of vitamin D₃ on the relative expression levels of interleukin 2 (IL-2) and interleukin 18 (IL-18) genes in tissues of Silky fowls. One hundred and twenty Silky fowls were randomly allotted to 5 treatments with 4 replicates in each treatment and 6 fowls per replicate. Fowls in control treatment were fed a basal diet, while those in experimental treatments were fed the basal diet supplemented with 800, 1 600, 3 200 and 6 400 IU/kg vitamin D₃, respectively. Fowls were fed for 6 weeks in cages. The tissues (thymus, duodenum, jejunum, ileum, cecum and bursa of Fabricius) were collected for the analysis of relative expression levels of IL-2 and IL-18 genes. The results showed as follows: 6 400 IU/kg vitamin D₃ up-regulated the relative expression level of IL-2 gene in tissues of duodenum, bursa of Fabricius and cecum, but no regulation was found in tissues of thymus, jejunum and ileum; 6 400 IU/kg vitamin D₃ up-regulated the relative expression level of IL-18 gene in tissues of thymus, ileum, cecum and bursa of Fabricius of Silky fowls. The results indicate that dietary supplemental level of vitamin D₃ can affect the relative expression levels of IL-2 and IL-18 genes in Silky fowls, but the effects are differ in different tissues.

Key words： Silky fowl; vitamin D₃; interleukin

参考文献

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