不同脂肪酸对体外培养的瘤胃微生物活力和蛋白质含量的影响

皮宇; 经语佳; 王梦芝; 王洪荣; 王慧; 黄蔚

doi:10.3969/j.issn.1006-267x.2014.01.034

动物营养学报 >

2014 , Vol. 26 >Issue 1: 260 - 269

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.01.034

研究简报 Short Communications

不同脂肪酸对体外培养的瘤胃微生物活力和蛋白质含量的影响

皮宇 ,
经语佳 ,
王梦芝 ,
王洪荣 ,
王慧 ,
黄蔚

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扬州大学动物科学与技术学院, 扬州 225009

收稿日期: 2013-07-01

网络出版日期: 2013-12-24

基金资助

江苏省自然科学基金项目（BK2011447）；江苏省优势学科建设项目

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Effects of Different Fatty Acids on in Vitro Rumen Microbial Activity and Protein Content

PI Yu ,
JING Yujia ,
WANG Mengzhi ,
WANG Hongrong ,
WANG Hui ,
HUANG Wei

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College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received date: 2013-07-01

Online published: 2013-12-24

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摘要

本试验旨在研究不同脂肪酸对体外培养的瘤胃微生物活力和蛋白质含量的影响。试验设6个分别添加3%硬脂酸、油酸、亚油酸、α-亚麻酸、花生四烯酸、二十碳五烯酸的试验组和1个添加3%棕榈酸钙的对照组，以3头瘘管山羊为试验动物提供瘤胃液进行体外培养。分别在培养开始后0、3、6、9、12、18、24 h采样，测定微生物活力和蛋白质含量相关指标。结果表明：油酸、亚油酸、α-亚麻酸、二十碳五烯酸组总脱氢酶活性均显著高于对照组和硬脂酸组（P<0.05）。油酸、亚油酸、α-亚麻酸、花生四烯酸、二十碳五烯酸组谷丙转氨酶和谷草转氨酶活性均显著高于对照组和硬脂酸组（P<0.05）。各组间pH的差异均不显著（P>0.05）。各组氨氮浓度随采样时间整体呈现先升高，再降低，最后升高的趋势，除了二十碳五烯酸组和对照组外，其他各组氨氮浓度在9 h时最低，组间则以α-亚麻酸组最低。6~18 h间α-亚麻酸组微生物蛋白质含量数值上均大于其他各组，部分组间差异显著（P<0.05）。综上，添加3%的不同脂肪酸对体外培养的瘤胃微生物活力和蛋白质含量具有一定的调控作用，且以α-亚麻酸效果较好。

关键词： 脂肪酸; 瘤胃微生物; 活力; 蛋白质

本文引用格式

皮宇 , 经语佳 , 王梦芝 , 王洪荣 , 王慧 , 黄蔚 . 不同脂肪酸对体外培养的瘤胃微生物活力和蛋白质含量的影响[J]. 动物营养学报, 2014 , 26(1) : 260 -269 . DOI: 10.3969/j.issn.1006-267x.2014.01.034

Abstract

This experiment was focused on the effects of different fatty acids on in vitro rumen microbial activity and protein content. Six experimental groups were stearic acid group, oleic acid group, linoleic acid group, α-linolenic acid group, arachidonic acid group and eicosapentaenoic acid group, which were added fatty acids at 3% level in the culture substrates, respectively, and one control group was added palmitic acid calcium at 3% level in the culture substrates. Three fistula goats provided rumen fluid in vitro. The samples were collected at 0, 3, 6, 9, 12, 18 and 24 h after culture to detect the microbial activity and protein content. The results showed that the total dehydrogenase activity in oleic acid, linoleic acid, α-linolenic acid and eicosapentaenoic acid groups were significantly higher than those in control group and stearic acid group (P<0.05). The glutamic-pyruvic transaminase and glutamic-oxal (o) acetic transaminase activities in oleic acid, linoleic acid, α-linolenic acid, arachidonic acid and eicosapentaenoic acid groups were significantly higher than those in control group and stearic acid group (P<0.05). No significant difference in pH was detected among all groups (P>0.05). In addition, ammonia nitrogen concentration was increased firstly and then decreased, and at last increased again. Expect for eicosapentaenoic acid and control groups, the ammonia nitrogen concentration was the lowest at 9 h in the other groups, with α-linolenic acid group showing the lowest data. The microbial protein content in α-linolenic acid group was significantly higher than that in the other groups from 6 to 18 h, and significant differences were found among part of groups (P<0.05). Taken together, different fatty acids at 3% level have certain regulatory effects on in vitro rumen microbial activity and protein content, and α-linolenic acid shows much better effects.

Key words： fatty acids; rumen microbes; activity; protein

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