硫胺素对高精料底物瘤胃体外发酵培养液挥发性脂肪酸和乳酸浓度的影响

张军; 王梦芝; 郝志敏; 喻礼怀; 王洪荣

doi:10.3969/j.issn.1006-267x.2014.02.026

动物营养学报 >

2014 , Vol. 26 >Issue 2: 489 - 495

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.02.026

饲料营养 Feed Science and Technology

硫胺素对高精料底物瘤胃体外发酵培养液挥发性脂肪酸和乳酸浓度的影响

张军 ,
王梦芝 ,
郝志敏 ,
喻礼怀 ,
王洪荣

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1. 扬州市扬大康源乳业有限公司, 扬州 225009;
2. 扬州大学实验农牧场, 扬州 225009;
3. 扬州大学动物科学与技术学院, 扬州 225009

收稿日期: 2013-08-19

网络出版日期: 2014-01-27

基金资助

国家自然科学基金项目（31072051）；江苏省自然科学基金项目（BK2011447）；江苏省优势学科

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Effects of Thiamine on Concentrations of Volatile Fatty Acids and Lactate in Culture Medium of High Concentrate Substrate after in Vitro Rumen Fermentation

ZHANG Jun ,
WANG Mengzhi ,
HAO Zhimin ,
YU Lihuai ,
WANG Hongrong

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1. Yangzhou Yangda Kangyuan Dairy Co., Ltd., Yangzhou 225009, China;
2. Yangzhou University Experimental Farm, Yangzhou 225009, China;
3. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received date: 2013-08-19

Online published: 2014-01-27

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摘要

本试验旨在研究高精料底物条件下添加硫胺素对瘤胃体外发酵培养液挥发性脂肪酸和乳酸浓度的影响。选择4头身体健康状况良好、安装永久性瘤胃瘘管的荷斯坦奶牛，用于瘤胃液的采集。以酪蛋白、小麦淀粉、可溶性淀粉、果胶、木聚糖、滤纸纤维素、羧甲基纤维素为原料，设计精粗比为6：4的发酵底物，硫胺素的添加量分别为0、60、120、180和240 mg/kg。结果表明，高精料底物条件下，添加硫胺素整体上降低了培养液乳酸的浓度，并以添加180 mg/kg硫胺素组效果较好，同时该组还提高了总挥发性脂肪酸的浓度，降低了乙酸与丙酸比例。综上所述，添加180 mg/kg的硫胺素能够较好地稳定高精料（6：4）底物的瘤胃发酵。

关键词： 硫胺素; 瘤胃发酵; 乳酸; 挥发性脂肪酸

本文引用格式

张军 , 王梦芝 , 郝志敏 , 喻礼怀 , 王洪荣 . 硫胺素对高精料底物瘤胃体外发酵培养液挥发性脂肪酸和乳酸浓度的影响[J]. 动物营养学报, 2014 , 26(2) : 489 -495 . DOI: 10.3969/j.issn.1006-267x.2014.02.026

Abstract

This experiment was conducted to study the effects of thiamine on concentrations of volatile fatty acids and lactate in culture medium of high concentrate substrate after in vitro rumen fermentation. Four healthy Holstein dairy cows equipped with permanent ruminal cannulas were used to provide rumen fluid. The fermentation substrate was consist of casein, wheat starches, soluble starches, pectins, xylan, milled filter paper and carboxymethyl cellulose, and the concentrate to forage ratio was 6：4. The supplemental levels of thiamine were 0, 60, 120, 180 and 240 mg/kg, respectively. The results showed as follows: generally, the lactate concentration in culture medium was decreased by thiamine under the condition of high concentrate substrate, and 180 mg/kg group showed the best effect. It was also observed that 180 mg/kg group increased total volatile fatty acid concentration but decreased the ratio of acetate to propionate. It is concluded that the supplemental level of 180 mg/kg thiamine can stabilize rumen microbial fermentation of high concentrate substrate with 6：4 of the concentrate to forage ratio.

Key words： thiamine; rumen fermentation; lactate; volatile fatty acid

参考文献

[1] MUTSVANGWA T, WRIGHT, T C.Sub-acute ruminal acidosis(SARA)in dairy cows[M].Ontario:Ministry of Agriculture and Food, 2003.

[2] COMBS G F, Jr.The vitamins:fundamental aspects in nutrition and health[J].American Journal of Clinical Nutrition, 1991, 53(3):755-763.

[3] SHAVER R D, BAL M A.Effect of dietary thiamin supplementation on milk production by dairy cows[J].Journal of Dairy Science, 2000, 83(10):2335-2340.

[4] 张红伟.硫胺素(VB₁)对瘤胃内营养物质消化代谢的影响[D].硕士学位论文.扬州:扬州大学, 2010.

[5] DE OLIVEIRA L A, JEAN-BLAIN C, KOMISARCZUK-BONY S, et al.Microbial thiamin metabolism in the rumen simulating fermenter:the effect of acidogenic conditions, a high sulfur level and added thiamin[J].British Journal of Nutrition, 1997, 78(4):599-613.

[6] 熊本海, 卢德勋, 高俊.绵羊瘤胃VFA吸收效率及模型参数的研究[J].动物营养学报, 1999, 11(S1):248-255.

[7] 张龙翔, 张庭芳, 李令媛.生化实验方法和技术[M].2版.北京:高等教育出版社, 1997:422-428.

[8] BECHDEL S I, HONEYWELL H E, DURCHER R A, et al.Synthesis of vitamin B in the rumen of the cow[J].Journal of Biological Chemistry, 1928, 80(1):231-238.

[9] MILLER B L, MEISKE J C, GOODRICH R D.Effect of grain source and concentrate level on B-vitamin production and absorption in steers[J].Journal of Animal Science, 1986, 62(2):473-483.

[10] ZINN R A, OWEN F N, STUART R L, et al.B-vitamin supplementation of diets for feedlot calves[J].Journal of Animal Science, 1987, 65(1):267-277.

[11] TAJIMA K, ARAI S, OGATA K, et al.Rumen bacterial community transition during adaptation to high-grain diet[J].Anaerobe, 2000, 6(5):273-284.

[12] 韩昊奇, 刘大程, 高民, 等.不同NFC/NDF比对奶山羊瘤胃微生物及瘤胃pH值变化的影响[J].动物营养学报, 2011, 25(4):597-603.

[13] PREVOT S, SENAUD J, BOHATIER J, et al.Variation in the composition of the ruminal bacterial microflora during the adaptation phase in an artificial fermentor (Rusitec)[J].Zoological Science, 1994, 11:871-878.

[14] 冯仰廉.反刍动物营养学[M].北京:科学出版社, 2004.

[15] VAN HOUTERT M.Challenging the retinal for altering VFA ratios in growing ruminates[J].Feed Mix, 1996, 4(1):8-12.

[16] BANNINK A, FRANCE J, LOPEZ S, et al.Modelling the implications of feeding strategy on rumen fermentation and functioning of the rumen wall[J].Animal Feed Science and Technology, 2008, 143(1/2/3/4):3-26.

[17] ØRSKOV E R, GRUBB D A, SMITH J S, et al.Efficiency of utilization of volatile fatty acids for maintenance and energy retention by sheep[J].British Journal of Nutrition, 1979, 41(3):541-552.

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