本试验旨在研究饲粮胆碱水平对3~5月龄獭兔生长性能、血清生化指标、盲肠发酵及毛皮质量的影响。选用200只体重相近、健康状况良好的3月龄獭兔,随机分成5组(每组40个重复,每个重复1只),分别饲喂胆碱实测水平为0.08%(A组)、0.09%(B组)、0.10%(C组)、0.11%(D组)和0.12%(E组)的试验饲粮。预试期7 d,正试期60 d。结果表明:饲粮胆碱水平对平均日采食量无显著影响(P>0.05),但极显著影响平均日增重(P<0.000 1)和料重比(P=0.000 2)。平均日增重和料重比在D组分别达到最高值和最低值,且均与A组差异极显著(P<0.01)。饲粮胆碱水平显著影响血清尿素氮(P=0.048 2)和总蛋白含量(P=0.011 7),而对谷丙转氨酶活性及甘油三酯、总胆固醇、高密度脂蛋白胆固醇和低密度脂蛋白胆固醇含量均无显著影响(P>0.05)。与A组相比,B、C和D组尿素氮含量显著降低(P<0.05),C、D和E组总蛋白含量显著降低(P<0.05)。饲粮胆碱水平极显著影响盲肠内容物pH(P<0.000 1)、总挥发性脂肪酸浓度(P<0.000 1)、乙酸比例(P=0.003 2)、乙酸/(丙酸+丁酸)(P=0.004 5),显著影响丙酸比例(P=0.030 7),但对丁酸比例和氨态氮浓度无显著影响(P>0.05)。与A组相比,C、D和E组pH极显著降低(P<0.01),D组总挥发性脂肪酸浓度极显著升高(P<0.01),D和E组乙酸比例极显著降低(P<0.01),C、D和E组丙酸比例显著升高(P<0.05),C、D和E组乙酸/(丙酸+丁酸)极显著升高(P<0.01)。饲粮胆碱水平极显著影响毛皮面积(P<0.000 1),显著影响毛皮重量(P=0.015 6),但对毛皮厚度和被毛长度无显著影响(P>0.05)。D和E组毛皮面积极显著高于A、B和C组(P<0.01),D组毛皮重量显著高于其他各组(P<0.05)。综合本试验测定指标,3~5月龄獭兔饲粮适宜的胆碱水平为0.11%。
This experiment was conducted to investigate the effects of dietary choline level on growth performance, serum biochemical indices, caecum fermentation and fur quality of 3 to 5-month-old Rex rabbits. Two hundred 3-month-old healthy Rex rabbits with similar body weight were randomly divided into 5 groups with 40 replicates in each group and 1 rabbit in each replicate. Rabbits in 5 groups were fed experimental diets which containing 0.08% (group A), 0.09% (group B), 0.10% (group C), 0.11% (group D) and 0.12% choline (group E), respectively. The choline levels in experimental diets were measured values. The experiment lasted for 7 days for adaptation, and 60 days for test. The results showed as follows: dietary choline level had no significant effect on average daily feed intake (ADFI) (P>0.05), but had significant effects on average daily gain (ADG) (P<0.000 1) and feed/gain (F/G) (P=0.000 2). The highest ADG and the lowest F/G were all found in group D, and there were significant differences in them between groups D and A (P<0.01). Dietary choline level had significant effects on the contents of serum urea nitrogen (P=0.048 2)and total protein (P=0.011 7), but had no significant effects on the activity of alanine aminotransferase and the contents of triglycerides, total cholesterol, high-density lipoprotein cholesterol and low-density lipoprotein cholesterol (P>0.05). Compared with the group A, the urea nitrogen content in groups B, C and D was significantly decreased (P<0.05), and the total protein content in groups C, D and E was significantly decreased (P<0.05). Dietary choline level had significant effects on cecal digesta pH (P<0.000 1), total volatile fatty acid concentration (P<0.000 1), acetic acid proportion (P=0.003 2), acetic acid/(propionic acid+butyric acid) (P=0.004 5) and propionic acid proportion (P=0.030 7), but had no significant effects on butyric acid proportion and ammonia nitrogen concentration (P>0.05). Compared with the group A, the pH in groups C, D and E was significantly decreased (P<0.01), the total volatile fatty acid concentration in group D was significantly increased (P<0.01), the acetic acid proportion in groups D and E was significantly decreased (P<0.01), the propionic acid proportion in groups C, D and E was significantly increased (P<0.05), and the acetic acid/(propionic acid+butyric acid) in groups C, D and E was significantly increased (P<0.01). Dietary choline level had significant effects on fur area (P<0.000 1) and fur weight (P=0.015 6), but had no significant effects on fur thickness and wool length (P>0.05). The fur area in groups D and E was significantly higher than that in groups A, B and C (P<0.01), and the fur weight in group D was significantly higher than that in other groups (P<0.05). Considering all indices of this experiment,the appropriate dietary choline level is 0.11% for 3 to 5-month-old Rex rabbits.
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