特种经济动物营养 Special Economic Animal Nutrition

低蛋白质饲粮中添加蛋氨酸对冬毛期蓝狐生产性能、营养物质消化率及氮代谢的影响

  • 郭俊刚 ,
  • 张铁涛 ,
  • 崔虎 ,
  • 高秀华 ,
  • 杨福合 ,
  • 邢秀梅
展开
  • 1. 中国农业科学院饲料研究所, 北京 100081;
    2. 中国农业科学院特产研究所, 吉林省特种经济动物分子生物学省部共建实验室, 长春 130112;
    3. 北京生泰尔 生物科技有限公司, 北京 102206

收稿日期: 2013-11-11

  网络出版日期: 2014-04-01

基金资助

公益性行业(农业)科研专项(200903014)

Effects of Methionine Supplementation in Low-Protein Diets on Performance, Nutrient Digestibility and Nitrogen Metabolism of Blue Foxes during the Winter Hair Period

  • GUO Jungang ,
  • ZHANG Tietao ,
  • CUI Hu ,
  • GAO Xiuhua ,
  • YANG Fuhe ,
  • XING Xiumei
Expand
  • 1. Institute of Feed Research, Chinese Academy of Agriculture Sciences, Beijing 100081, China;
    2. State Key Laboratory of Special Economic Animal Molecular Biology, Institute of Special Animal and Plant Science, Chinese Academy of Agriculture Sciences, Changchun 130112, China;
    3. Beijing Centre Biology Co., Ltd., Beijing 102206, China

Received date: 2013-11-11

  Online published: 2014-04-01

摘要

本试验旨在研究低蛋白质饲粮中添加蛋氨酸对冬毛期蓝狐生产性能、营养物质消化率及氮代谢的影响。选用25周龄健康雄性蓝狐105只,平均体重为(4.88±0.60)kg,随机分为7组,每组15个重复,每个重复1只。正对照组饲喂蛋白质水平为28%的基础饲粮,负对照组饲喂蛋白质水平为26%的基础饲粮,试验组分别饲喂在低蛋白质饲粮(蛋白质水平为26%的基础饲粮)基础上添加0.2%、0.4%、0.6%、0.8%和1.0%蛋氨酸的试验饲粮。预试期7 d,正试期80 d。结果表明:0.6%蛋氨酸组终末体重、平均日增重和饲料转化率极显著高于负对照组和0.2%蛋氨酸组(P<0.01),并且饲料转化率显著高于0.4%、0.8%、1.0%蛋氨酸组和正对照组(P<0.05);0.6%蛋氨酸组干皮长显著长于0.2%、0.4%、0.8%蛋氨酸组和负对照组(P<0.05),并与正对照组差异不显著(P>0.05)。0.6%蛋氨酸组干物质消化率、粗蛋白质消化率及粗脂肪消化率与正对照组相比差异不显著(P>0.05),但显著或极显著高于负对照组(P<0.05或P<0.01);1.0%蛋氨酸组半胱氨酸消化率和蛋氨酸消化率显著或极显著高于其他各组(P<0.05或P<0.01)。0.6%蛋氨酸组氮沉积极显著高于0.2%、0.4%、1.0%蛋氨酸组及负对照组(P<0.01),与正对照组相比差异不显著(P>0.05);0.6%蛋氨酸组净蛋白质利用率和蛋白质生物学价值显著高于1.0%蛋氨酸组和负对照组(P<0.05),与正对照组相比差异不显著(P>0.05)。综合本试验各项测定指标,在蛋白质水平为26%的低蛋白质饲粮中添加0.6%的蛋氨酸,即饲粮中蛋氨酸水平为0.99%时,能够满足冬毛期蓝狐对蛋氨酸的需要量,此时蓝狐的生长性能、营养物质消化率、氮沉积和相应的毛皮参数较为理想。

本文引用格式

郭俊刚 , 张铁涛 , 崔虎 , 高秀华 , 杨福合 , 邢秀梅 . 低蛋白质饲粮中添加蛋氨酸对冬毛期蓝狐生产性能、营养物质消化率及氮代谢的影响[J]. 动物营养学报, 2014 , 26(4) : 996 -1003 . DOI: 10.3969/j.issn.1006-267x.2014.04.019

Abstract

This experiment was conducted to study the effects of methionine supplementation in low-protein diets on performance, nutrient digestibility and nitrogen metabolism of blue foxes during the winter hair period. One hundred and five healthy male blue foxes at the age of 25 weeks with an average body weight of (4.88±0.60) kg were randomly assigned into 7 groups with 15 replicates per group and 1 fox per replicate. The blue foxes in positive control group were fed a 28% protein level basal diet, and those in negative control group were fed a 26% protein level basal diet. The blue foxes in experimental groups were fed low-protein diets (26% protein level basal diets) supplemented with 0.2%, 0.4%, 0.6%, 0.8% and 1.0% methionine, respectively. The pre-test lasted for 7 days and the formal test lasted for 80 days. The results showed as follows: the final body weight, average daily gain and feed conversion rate in 0.6% methionine group were extremely significantly higher than those in negative control group and 0.2% methionine group (P<0.01), and the feed conversion rate was significantly higher than that in 0.4%, 0.8% and 1.0% methionine groups and positive control group (P<0.05). The length of dry fur in 0.6% methionine group was not significantly different compared with positive control group (P>0.05), but significantly longer than that in 0.2%, 0.4% and 0.8% methionine groups and positive control group (P<0.05). The digestibility of dry matter, crude protein and ether extract in 0.6% methionine group was not significantly different compared with positive control group (P>0.05), but significantly or extremely significantly higher than that in negative control group (P<0.05 or P<0.01); the digestibility of cysteine and methionine in 1.0% methionine group was significantly or extremely significantly higher than that in other groups (P<0.05 or P<0.01). The nitrogen deposition in 0.6% methionine group was not significantly different compared with positive control group (P>0.05), but extremely significantly higher than that in 0.2%, 0.4% and 1.0% methionine groups and negative control group (P<0.01); the net protein utilization and protein biological value in 0.6% methionine group were not significantly different compared with positive control group (P>0.05), but significantly higher than those in 1.0% methionine group and negative control group (P<0.05). In conclusion, considering all measured indices of this experiment, when 0.6% methionine is supplemented in the low-protein diet with 26% protein level, that is, when the dietary methionine level is 0.99%, methionine requirement of blue foxes during the winter hair period can be met. In this case, the blue foxes have the optimum growth performance, nutrient digestibility, nitrogen deposition and relevant fur parameters.

参考文献

[1] DAHLMAN T,VALAJA J,NIEMELÄ P,et al.Influence of protein level and supplementary L-methionine and lysine on growth performance and fur quality of blue fox (Alopex lagopus)[J].Acta Agriculture Scandinavica,Section A:Animal Science,2002,52(4):174-182.  

[2] 崔虎,张铁涛,高秀华,等.饲粮蛋白质水平对育成期蓝狐生长性能及营养物质消化代谢的影响[J].动物营养学报,2011,23(8):1439-1445.

[3] 崔虎,张铁涛,张志强,等.饲粮蛋白质水平对冬毛期蓝狐生长性能、营养物质消化代谢及血清生化指标的影响[J].动物营养学报,2011,23(12):2217-2224.

[4] 张铁涛,崔虎,杨颖,等.饲粮蛋白质水平对育成期母貂生长性能、营养物质消化代谢及血清生化指标的影响[J].动物营养学报,2012,24(5):835-844.

[5] SKREDE A.Utilization of fish and animal byproducts in mink nutrition. Ⅳ. Fecal excretion and digestibility of nitrogen and amino acids by mink fed cod (Gadus morrhua) fillet or meat-and-bone meal[J].Acta Agriculture Scandinavica,1979,29(3):241-257.  

[6] DAHLMAN T.Protein and amino acids in the nutrition of the growing-furring blue fox [D].Ph.D.Thesis.Finland:University of Helsinki,2003:33.

[7] 张铁涛,崔虎,高秀华,等.低蛋白质饲粮中添加蛋氨酸对育成期蓝狐生长性能和营养物质消化代谢的影响[J].动物营养学报,2013,25(9):2036-2043.

[8] NRC.Nutrient requirements of mink and foxes[S].Washington,D.C.:National Academy Press,1982.

[9] 全国饲料工业标准化技术委员会.GB/T 6435—2006饲料中水分和其他挥发性物质含量的测定[S].北京:中国标准出版社,2006.

[10] 全国饲料工业标准化技术委员会.GB/T 6433—2006饲料中粗脂肪测定方法[S].北京:中国标准出版社,2006.

[11] 全国饲料工业标准化技术委员会.GB/T 6432—1994饲料中粗蛋白测定方法[S].北京:中国标准出版社,1994.

[12] 刘景,方桂友,董志岩,等.不同赖氨酸水平的低蛋白日粮对肥育猪生产性能、血清尿素氮及游离氨基酸水平的影响[J].福建畜牧兽医,2012,34(5):5-7.

[13] ABE M,OKADA H,MATSUMURA D,et al.Methionin embalance and toxicity in calves[J].Journal of Animal Science,2000,78(10):2722-2730.

[14] 谢明,侯水生,刘福柱.蛋氨酸毒性及其中毒机制的研究进展[J].饲料博览,2002(4):14-15.

[15] LIU S M,MATA G,FIGLIOMENI S,et al.Transsulfuration, protein synthesis rate and follicle mRNA in the skin of young Merino lambs in response to infusions of methionine and serine[J].British Journal of Nutrition,2000,83(4):401-409.

[16] TYÖPPÖNEN J,BERG H,VALTONEN M.Effects of dietary supplement of methionine and lysine on blood parameters and fur quality in mink fed with low-protein diets[J].Acta Agriculture Scandinavica,1987,37(4):487-494.  

[17] 张铁涛,张志强,高秀华,等.冬毛生长期公貂对不同蛋白质水平日粮营养物质消化率及氮代谢的比较研究[J].动物营养学报,2010,22(3):723-728.

[18] STRYER L.Biochemistry[M].New York:W.H. Freeman and Company,1998.

[19] RØSJØ C,NORDRUM S,OLLI J J,et al.Lipid digestibility and metabolism in Atlantic salmon (Salmo salar) fed medium-chain triglycerides[J].Aquaculture,2000,190(1/2):65-76.

[20] MUTT V.Historical perspectives on cholecystokinin research[J].Annals of the New York Academy of Sciences,1994,713(1):1-10.

[21] DAHLMAN T,BLOMSTEDT L.Effect of feed protein level on fur and skin of the blue fox[J].Scientifur,2000,24(4):13-16.

[22] 董志岩,刘景,叶鼎承等.不同低蛋白日粮添加氨基酸对生长猪生长性能及血液生化指标的影响[J].福建农业学报,2009,24(4):341-344.

[23] 杨春花,邹兴淮,张贵权.精料的蛋白质水平及能量浓度对成体大熊猫日粮消化率的影响[J].林业科学,2005,41(6):119-125.

[24] 张海华,李光玉,杨福合,等.低蛋白质日粮中添加赖氨酸和蛋氨酸对生长前期蓝狐消化代谢及生产性能的影响[J].动物营养学报,2008,20(6):23-29.
文章导航

/