本试验旨在研究黄芪多糖（APS）对肉仔鸡血清免疫细胞因子含量及小肠诱导型一氧化氮合成酶（iNOS）mRNA表达的影响。试验选择1日龄健康艾维茵肉仔鸡300只，随机分为5个组，每组6个重复，每个重复10只鸡。对照组饲喂基础饲粮，试验组分别在基础饲粮中添加0.1%、0.5%、1.0%和2.0%的APS。试验期42 d。结果表明：1）饲粮中添加APS可提高肉仔鸡的平均体重和平均日增重，降低料重比，其中以1.0%APS组效果最佳。2）饲粮中添加APS可提高肉仔鸡血清白细胞介素-1、白细胞介素-2和肿瘤坏死因子-α含量，42日龄血清白细胞介素-1和肿瘤坏死因子-α含量呈显著二次曲线增加（P<0.05），二者分别以1.0%和0.5%APS组最高。3）饲粮中添加APS可提高肉仔鸡血清一氧化氮含量，增加iNOS活性，促进小肠iNOS mRNA的表达，42日龄时小肠iNOS mRNA的表达呈显著二次曲线增加（P<0.05），其中以1.0%APS组效果最佳。由此可见，APS对肉仔鸡免疫功能的调节作用可能与iNOS mRNA的表达和活性以及免疫细胞因子的分泌有关。
This experiment was conducted to study the effects of Astragalus polysaccharides (APS) on serum immunocyte factor contents and small intestine inducible nitric oxide synthase (iNOS) mRNA expression of broilers. A total of 300 one-day-old healthy Avian broilers were randomly divided into 5 groups with 6 replicates per group and 10 broilers per replicate. Broilers in the control group were fed a basal diet, and the others in the experimental groups were fed the basal diet supplemented with 0.1%, 0.5%, 1.0% and 2.0% APS, respectively. The experiment lasted for 42 days. The results showed as follows: 1) dietary supplementation of APS increased the average weight and the average daily gain of broilers, and decreased the ratio of feed to gain, and the 1.0% APS group had the best effect. 2) Dietary supplementation of APS increased the contents of interleukin-1, interleukin-2 and tumor necrosis factor-α in serum of broilers, the contents of interleukin-1 and tumor necrosis factor-α in serum showed a quadratic increasing at 42 days of age (P<0.05), and the contents of tumor necrosis factor-α and interleukin-1 in serum in 0.5% and 1.0% APS groups had the best effect at 42 days of age, respectively. 3) Dietary supplementation of APS increased the nitric oxide content and iNOS activity in serum, and increased the small intestine iNOS mRNA expression, the small intestine iNOS mRNA expression showed a quadratic increasing at 42 days of age (P<0.05), and the 1.0% APS group had the best effect. In conclusion, the regulation of APS on immune function of broilers may relate to the expression of iNOS mRNA, iNOS activity and the secretion of immunocyte factors.
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