饲料安全 Feed Security

转基因豆粕对崂山奶山羊生长性能、肌肉营养成分及组织器官中外源基因转移的影响

  • 刘斌 ,
  • 秦志华 ,
  • 黄娟 ,
  • 张廷荣 ,
  • 刘文达 ,
  • 王述柏
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  • 青岛农业大学动物科技学院, 青岛 266109

收稿日期: 2013-11-11

  网络出版日期: 2014-04-01

基金资助

动物卫生风险分析关键技术与应用研究(200903055)

Effects of Genetically Modified Soybean Meal on Growth Performance, Meat Nutrient Composition, and Exogenous Gene Transformation to Tissues and Organs of Laoshan Dairy Goats

  • LIU Bin ,
  • QIN Zhihua ,
  • HUANG Juan ,
  • ZHANG Tingrong ,
  • LIU Wenda ,
  • WANG Shubai
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  • College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China

Received date: 2013-11-11

  Online published: 2014-04-01

摘要

本试验旨在研究转基因豆粕对崂山奶山羊生长性能、肌肉营养成分及组织器官中外源基因转移的影响。选取30只50日龄崂山奶山羊随机分为2组,每组3个重复,每个重复5只。精饲料分别以非转基因豆粕和转基因豆粕(MON89788和GTS40-3-2)配制,粗饲料均为干草。试验期150 d。结果表明:2组奶山羊平均日采食量、平均日增重和料重比均无显著差异(P>0.05);奶山羊肌肉的干物质、粗脂肪、粗蛋白质、粗灰分、钙、磷含量2组间亦均无显著差异(P>0.05);采用实时定量PCR法检测奶山羊肌肉、肝脏、胰腺、肾脏、脾脏、胸腺、肺、心脏、皱胃、小肠组织的外源基因,均未检测出转基因片段。可见,转基因豆粕(MON89788和GTS40-3-2)对崂山奶山羊生长性能和肌肉品质均无显著影响,转基因豆粕的外源基因在奶山羊肌肉及各组织器官未发生水平转移。

本文引用格式

刘斌 , 秦志华 , 黄娟 , 张廷荣 , 刘文达 , 王述柏 . 转基因豆粕对崂山奶山羊生长性能、肌肉营养成分及组织器官中外源基因转移的影响[J]. 动物营养学报, 2014 , 26(4) : 1028 -1033 . DOI: 10.3969/j.issn.1006-267x.2014.04.023

Abstract

In order to analyze the effects of genetically modified soybean meal on growth performance, meat nutrient composition, and exogenous gene transformation to tissues and organs of Laoshan dairy goats, thirty 50-day-old Laoshan dairy goats were randomly divided into two groups with three replicates in each group and five goats per replicate. Goats in different groups were fed concentrates containing non-genetically modified soybean meal and genetically modified soybean meal (MON89788 and GTS40-3-2), respectively, and were fed the same roughage (Chinese wildrye). The experiment lasted for 150 days. The results showed as follows: average daily feed intake, average daily gain and feed to gain ratio in the two groups were not significantly different (P>0.05); the contents of dry matter, ether extract, crude protein, ash, calcium and phosphorus in muscle of dairy goats were not significantly different either (P>0.05). Moreover, no gene ragments of genetically modified soybean meal were detected from muscle, liver, pancreas, kidney, spleen, thymus, lung, heart, abomasum and small intestine of dairy goats by real-time quantification PCR. The results suggest that genetically modified soybean meal (MON89788 and GTS40-3-2) had no significant effects on growth performance and muscle quality of Laoshan dairy goats, and no genetic horizontal transformation is observed in tissues and organs.

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