精氨酸和N-氨甲酰谷氨酸对环江香猪脂质代谢的影响

周笑犁; 刘俊锋; 吴琛; 耿梅梅; 孔祥峰

doi:10.3969/j.issn.1006-267x.2014.04.026

动物营养学报 >

2014 , Vol. 26 >Issue 4: 1055 - 1060

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.04.026

研究简报 Short Communications

精氨酸和N-氨甲酰谷氨酸对环江香猪脂质代谢的影响

周笑犁 ,
刘俊锋 ,
吴琛 ,
耿梅梅 ,
孔祥峰

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1. 中国科学院亚热带农业生态研究所, 亚热带农业生态过程重点实验室, 湖南省畜禽健康养殖工程技术研究中心, 长沙 410125;
2. 贵阳学院, 贵阳 550005;
3. 中国科学院环江喀斯特农业生态试验站香猪研究中心, 环江 547100

收稿日期: 2013-10-10

网络出版日期: 2014-04-01

基金资助

国家973计划课题（2012CB124704、2013CB127305）；国家自然科学基金项目（31270044、31101729）

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Effects of Arginine and N-Carbamylglutamate on Lipid Metabolism of Huanjiang Mini-Pigs

ZHOU Xiaoli ,
LIU Junfeng ,
WU Chen ,
GENG Meimei ,
KONG Xiangfeng

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1. Key Laboratory for Agro-Ecological Processes in Subtropical Region, Hunan Engineering and Research Center of Animal and Poultry Science, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;
2. Guiyang University, Guiyang 550005, China;
3. Research Center of Mini-Pig, Huanjiang Observation and Research Station for Karst Ecosystems, Chinese Academy of Sciences, Huanjiang 547100, China

Received date: 2013-10-10

Online published: 2014-04-01

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摘要

为了研究饲粮添加L-精氨酸（Arg）或N-氨甲酰谷氨酸（NCG）对环江香猪肌肉脂肪酸含量和脂质代谢相关基因表达的影响，选用初始体重为（28.14±3.45）kg的环江香猪24头，随机分为3组，每组4个重复，每个重复2头猪。Arg组和NCG组分别在基础饲粮中添加0.83%L-Arg+0.88%米糠和0.08%NCG+1.71%L-丙氨酸，对照组在基础饲粮中添加1.71%L-丙氨酸。饲喂45 d后称取体重，放血处死，采集肝脏和背最长肌，测定背最长肌中脂肪酸的含量、肝脏和背最长肌中脂质代谢相关基因的表达。结果表明，与对照组相比，饲粮添加Arg可显著降低肌肉C18：0含量（P<0.05），显著下调肝脏乙酰CoA羧化酶α（ACCα）以及肌肉ACCα和过氧化物酶增生物激活受体γ（PPARγ）mRNA表达（P<0.05），显著上调肝脏PPARγ mRNA表达（P<0.05）；饲粮添加NCG可显著下调肝脏ACCα和脂蛋白脂酶（LPL）以及肌肉ACCα、脂肪酸合成酶（FAS）和LPL mRNA表达（P<0.05），显著上调肌肉PPARγ mRNA表达（P<0.05）。上述结果提示，饲粮添加Arg或NCG可调节肝脏和背最长肌中脂质代谢相关基因的表达，并降低背最长肌中C18：0的含量。

关键词： 精氨酸; N-氨甲酰谷氨酸; 环江香猪; 脂肪酸; 脂质代谢

本文引用格式

周笑犁 , 刘俊锋 , 吴琛 , 耿梅梅 , 孔祥峰 . 精氨酸和N-氨甲酰谷氨酸对环江香猪脂质代谢的影响[J]. 动物营养学报, 2014 , 26(4) : 1055 -1060 . DOI: 10.3969/j.issn.1006-267x.2014.04.026

Abstract

This study was conducted to determine the effects of dietary supplemented with L-arginine (Arg) or N-carbamylglutamate (NCG) on intramuscular contents of fatty acids and the gene expression levels related to lipid metabolism in Huanjiang mini-pigs. Tweety-four Huanjiang mini-pigs with initial body weight (BW) of (28.14±3.45) kg were randomly assigned into 3 groups with 4 replicates in each group and 2 pigs per replicate, representing Arg, NCG and control groups, and pigs were supplemented with 0.83% L-Arg + 0.88% rice bran, 0.08% NCG+1.71% L-alanine, and 1.71% L-alanine in a basal diet, respectively. After a 45-day feeding period, the liver and longissimus dorsi were obtained for determining intramuscular contents of fatty acids and the gene expression levels related to lipid metabolism. The results showed that compared with the control group, dietary supplemented with Arg significantly decreased muscle content of C18:0 (P<0.05), as well as the expression levels of acetyl-CoA carboxylase α (ACCα) mRNA in liver, and ACCα and peroxisome proliferator activated receptor-γ (PPARγ) mRNA in muscle (P<0.05), while significantly increased the expression level of PPARγ mRNA in liver (P<0.05); dietary supplementation of NCG significantly decreased the expression levels of ACCα and lipoprteinlipase (LPL) mRNA in liver (P<0.05), as well as ACCα, fatty acid synthetase (FAS) and LPL mRNA in muscle, while significantly increased the expression level of PPARγ mRNA in muscle (P<0.05). These results indicate that dietary supplemented with Arg or NCG have a regulating effect on gene expression related to lipid metabolism in the liver and longissimus dorsi, and decrease the content of C18:0 in longissimus dorsi.

Key words： arginine; N-carbamylglutamate; Huanjiang mini-pig; fatty acid; lipid metabolism

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