反刍动物营养 Ruminant Nutrition

真胃灌注不同水平淀粉对泌乳后期奶牛采食量、泌乳性能、胃肠道发酵和血液代谢的影响

  • 邹杨 ,
  • 李胜利 ,
  • 曹志军 ,
  • 杨占山
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  • 中国农业大学动物科技学院, 动物营养国家重点实验室, 北京 100193

收稿日期: 2013-12-01

  网络出版日期: 2014-05-30

基金资助

国家自然科学基金项目(31372334)

Effects of Graded Abomasal Starch Infusion on Feed Intake, Lactation Performance, Gastrointestinal Fermentation and Blood Metabolism of Lactating Cows

  • ZOU Yang ,
  • LI Shengli ,
  • CAO Zhijun ,
  • YANG Zhanshan
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  • State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China

Received date: 2013-12-01

  Online published: 2014-05-30

摘要

为了研究真胃灌注不同水平淀粉对泌乳后期奶牛采食量、泌乳性能、胃肠道发酵和血液代谢的影响,采用4×4拉丁方设计将4头安装有永久性瘤胃瘘管的荷斯坦2胎奶牛分为4组,分别向真胃内灌注0(对照组)、800、1 600、2 400 g/d淀粉。共进行4期试验,每期7 d(5 d的预试期和2 d的正试期)。结果表明:1)真胃灌注不同水平淀粉对奶牛干物质采食量无显著影响(P>0.05);奶牛的消化能(P=0.022)和泌乳净能采食量(P=0.014)均随着真胃淀粉灌注量的增加而呈线性增加,而奶牛的淀粉表观消化率则呈线性降低(P=0.029)。2)随着真胃淀粉灌注量的增加,乳脂率(P=0.024)和乳脂产量(P=0.022)均呈二次曲线上升,以1 600 g/d灌注量时最大。3)800 g/d组的粪液丁酸浓度显著低于1 600和2 400 g/d组(P0.05)。综合以上,乳脂率随着真胃淀粉灌注量的增加呈二次曲线上升;泌乳后期奶牛能够利用的过瘤胃淀粉的最大量为1 600 g/d。

本文引用格式

邹杨 , 李胜利 , 曹志军 , 杨占山 . 真胃灌注不同水平淀粉对泌乳后期奶牛采食量、泌乳性能、胃肠道发酵和血液代谢的影响[J]. 动物营养学报, 2014 , 26(6) : 1467 -1476 . DOI: 10.3969/j.issn.1006-267x.2014.06.006

Abstract

This experiment was performed to assess the effects of infusion of graded starch in abomasum on feed intake, lactation performance, gastrointestinal fermentation and blood metabolism of lactating cows. Four Holstein cows fitted with permanent ruminal cannulas were arranged into four groups by a 4×4 Latin square design. Cows were infused with 0, 800, 1 600 and 2 400 g/d starch in abomasum, respectively. The experiment lasted for 7 days, which was consisted of a 5-day pre-experimental period and a 2-day experimental period. The results showed as follows: 1) dry matter intake of dairy cows was not affected by abomasal starch infusion at different levels (P>0.05); with the increase of abomasal starch infusion level, digestive energy intake (P=0.022) and net energy for lactation intake (P=0.014) were linearly increased, while starch apparent digestibility was linearly decreased (P=0.029). 2) With the increase of abomasal starch infusion level, milk fat percentage (P=0.024) and yield (P=0.022) were quadratically increased, those in 1 600 g/d group reached the highest. 3) Butyric acid concentration in fecal fluid in 800 g/d group was significantly lower than that in 1 600 and 2 400 g/d groups (P0.05). In conclusion, milk fat percentage is quadratically increased with the increase of abomasal starch infusion level, and the peak amount of rumen escaped starch that can be utilized by dairy cows at late lactation period is 1 600 g/d.

参考文献

[1] YOUNG J W.Gluconeogenesis in cattle:significance and methodology[J].Journal of Dairy Science,1977,60(1):1-15.  
[2] REYNOLDS C K,HARMON D L,CECAVA M J.Absorption and delivery of nutrients for milk protein synthesis by portal-drained viscera[J].Journal of Dairy Science,1994,77(9):2787-2808.  
[3] HUNTINGTON G B,REYNOLDS P J.Net absorption of glucose,L-lactate,volatile fatty acids,and nitrogenous compounds by bovine given abomasal infusions of starch or glucose[J].Journal of Dairy Science,1986,69(9):2428-2436.  
[4] KREIKEMEIER K K,HARMON D L,BRANDT,R T,et al.Small intestinal starch digestion in steers:effect of various levels of abomasal glucose, corn starch and corn dextrin infusion on small intestinal disappearance and net glucose absorption[J].Journal of Animal Science,1991,69(1):328-338.
[5] REYNOLDS C K,HUMPHRIES D J,CAMMELL S B,et al.Effects of abomasal wheat-starch infusion on splanchnic metabolism and energy-balance of lactating dairy-cows[M]//MCCRACKEN K,UNSWORTH E F,WYLIE A R G.Energy metabolism of farm animals.New York:CAB International,1998:39-42.
[6] OWENS F N,ZINN R A,KIM Y K.Limits to starch digestion in the ruminant small intestine[J].Journal of Animal Science,1986,63(5):1634-1648.
[7] HUNTINGTON G B.Starch utilization by ruminants:from basics to the bunk[J].Journal of Animal Science,1997,75(3):852-867.
[8] ARMSTRONG D A,BLAXTER K L,GRAHAM N M.Fat synthesis from glucose by sheep [C]//Proceedings of the nutrition society.Oxon:CAB International,1960,19(2):R31-R32.
[9] MCDONALD P,EDWARDS R A,GREENHALGH J F D,et al.Animal nutrition[M].5th ed.Harlow:Longman Scientific & Technical,1995.
[10] ØRSKOV E R,FRASER C,KAY R N B.Dietary factors influencing the digestion of starch in the rumen and small and large intestine of early weaned lambs[J].British Journal of Nutrition,1969,23(2):217-226.  
[11] TYRRELL H F,MOE P W.Net energy value of a corn and a barley ration for lactation[J].Journal of Dairy Science,1974,57(4):451-458.  
[12] NOCEK J E,TAMMINGA S.Site of digestion of starch in the gastrointestinal tract of dairy cows and its effect on milk yield and composition[J].Journal of Dairy Science,1991,74(10):3598-3629.  
[13] THEURER C B,HUBER J T,DELGADO-ELORDUY A,et al.Invited review:summary of steam-flaking corn or sorghum grain for lactating dairy cows[J].Journal of Dairy Science,1999,82(9):1950-1959.  
[14] BOUDON A,FAVERDIN P,DELAGARDE R,et al.Effects of rumen or duodenal glucose infusions on intake in dairy cows fed fresh perennial ryegrass indoors[J].Journal of Dairy Science,2007,90(9):4397-4410.  
[15] KNOWLTON K F,DAWSON T E,GLENN B P,et al.Glucose metabolism and milk yield of cows infused abomasally or ruminally with starch[J].Journal of Dairy Science,1980,81(12):3248-3258.
[16] LARSEN M,KRISTENSEN N B.Effect of abomasal glucose infusion on splanchnic and whole-body glucose metabolism in periparturient dairy cows[J].Journal of Dairy Science,2009,92(3):1071-1083.  
[17] WILDMAN E E,JONES G M,WAGNER P E,et al.A dairy cow body condition scoring system and its relationship to selected production characteristics[J].Journal of Dairy Science,1982,65(3):495-501.  
[18] MCCLEARY B V,GIBSON T S,MUGFORD D C.Measurement of total starch in cereal products by amyloglucosidase-alpha-amylase method:collaborative study[J].Journal of AOAC International,1997,80(3):571-579.
[19] VAN KEULEN J,YOUNG B A.Evaluation of acid-insoluble ash as a natural marker in ruminant digestibility studies[J].Journal of Animal Science,1977,44(2):282-287.
[20] 曹锦萍,张晓雷,翟英丽,等.全收粪法和AIA指示剂法测定奶牛日粮有机物消化率差异比较[J].中国草食动物,2008,28(6):18-20.
[21] DAHLQVIST A.A method for the determination of amylase in intestinal content[J].Scandinavian Journal of Clinical and Laboratory Investigation,1962,14(2):145-151.  
[22] HAMADA T,OMORI S,KAMEOKA K,et al.Direct determination of rumen volatile fatty acids by gas chromatography[J].Journal of Dairy Science,1968,51(2):228-229.  
[23] BRODERICK G A,KANG J H.Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media[J].Journal of Dairy Science,1980,63(1):64-75.  
[24] BERG E L,FU C J,PORTER J H,et al.Fructooligosaccharide supplementation in the yearling horse:effects on fecal pH,microbial content,and volatile fatty acid concentrations[J].Journal of Animal Science,2005,83(7):1549-1553.
[25] OSBORNE J K,MUTSVANGWA T,ALZAHAL O,et al.Effects of monensin on ruminal forage degradability and total tract diet digestibility in lactating dairy cows during grain-induced subacute ruminal acidosis[J].Journal of Dairy Science,2004,87(6):1840-1847.  
[26] REYNOLDS C K,SUTTON J D,BEEVER D E.Effects of feeding starch to dairy-cattle on nutrient availability and production [C]//Recent advances in animal nutrition.Nottingham:Nottingham University Press,1997:103-134.
[27] HURTAUD C,RULQUIN H,VÉRITÉ R.Effects of graded duodenal infusions of glucose on yield and composition of milk from dairy cows.1.Diets based on corn silage[J].Journal of Dairy Science,1998,81(12):3239-3247.  
[28] REYNOLDS C K,CAMMELL S B,HUMPHRIES D J,et al.Effects of postrumen starch infusion on milk production and energy metabolism in dairy cows[J].Journal of Dairy Science,2001,84(10):2250-2259.  
[29] LAWHON S D,MAURER R,ALTIER C,et al.Intestinal short-chain fatty acids alter salmonella typhmurium invasion gene expression and virulence through BarA/SirA[J].Molecular Microbiology,2002,46(5):1451-1464.  
[30] VERNON R G.Lipid metabolism in the adipose tissue of ruminant animals[J].Progress in Lipid Research,1980,19(1/2):23-106.
[31] MCCLYMONT G L,VALLANCE S.Depression of blood glycerides and milk-fat synthesis by glucose infusion [C]//Proceedings of the nutrition society.Oxon:CAB International,1962,21(2):R41.
[32] HURTAUD C,LEMOSQUET S,RULQUIN H.Effect of graded duodenal infusions of glucose on yield and composition of milk from dairy cows.2.Diets based on grass silage[J].Journal of Dairy Science,2000,83(12):2952-2962.  
[33] GRIINARI J M,MCGUIRE M A,DWYER D A,et al.Role of insulin in the regulation of milk fat synthesis in dairy cows[J].Journal of Dairy Science,1997,80(6):1076-1084.  
[34] LEMOSQUET S,RIDEAU N,RULQUIN H,et al.Effects of a duodenal glucose infusion on the relationship between plasma concentration of glucose and insulin in dairy cows[J].Journal of Dairy Science,1997,80(11):2854-2865.  
[35] GRUMMER R R.Impact of changes in organic nutrient metabolism on feeding the transition dairy cow[J].Journal of Animal Science,1995,73(9):2820-2833.
[36] PEEL C J,FRONK T J,BAUMAN D E,et al.Lactational response to exogenous growth hormone and abomasal infusion of a glucose-sodium caseinate mixture in high-yielding dairy cows[J].The Journal of Nutrition,1982,112(9):1770-1778.
[37] VIK-MO L,EMERY R S,HUBER J T.Milk protein production in cows abomasally infused with casein or glucose[J].Journal of Dairy Science,1974,57(8):869-877.  
[38] HURTAUD C,VRIT R,RULQUIN H.Effects on milk yield and composition of infusions of different levels and natures of energy into the digestive tract of dairy cows[J].Annales de Zootechnie(Paris),1992,41(1):105.
[39] HURTAUD C,VRIT R,RULQUIN H.Effects on milk yield and composition of infusions of graded levels of glucose into the duodenum of dairy cows[J].Annales de Zootechnie(Paris),1994,43(3):309.
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