动物营养学报 >

2014 , Vol. 26 >Issue 6: 1527 - 1534

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.06.013

分子营养 Molecular Nutrition

乙酸钠和β-羟丁酸钠对奶牛乳腺上皮细胞乳脂和乳蛋白合成相关基因表达的影响

  • 塔娜 ,
  • 李红磊 ,
  • 侯先志 ,
  • 考桂兰 ,
  • 高民 ,
  • 李大彪
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  • 1. 内蒙古农业大学动物科学学院, 呼和浩特 010018;
    2. 内蒙古农业大学兽医学院, 呼和浩特 010018;
    3. 内蒙古农牧业科学院动物营养研究所, 呼和浩特 010031

收稿日期: 2013-12-05

  网络出版日期: 2014-05-30

基金资助

国家自然科学基金“应用三维细胞培养模型研究脂肪酸和激素对奶牛乳腺上皮细胞乳脂肪合成的影响及分子机理”（31360559）；现代农业（奶牛）产业技术体系建设专项资金（CARS-37）

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Effects of Sodium Acetate and Sodium β-Hydroxybutyrate on Expressions of Genes Involved in Milk Fat and Protein Synthesis in Bovine Mammary Epithelial Cells

  • TA Na ,
  • LI Honglei ,
  • HOU Xianzhi ,
  • KAO Guilan ,
  • GAO Min ,
  • LI Dabiao
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  • 1. College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China;
    2. College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China;
    3. Institute of Animal Nutrition, Inner Mongolia Academy of Agriculture and Animal Husbandry Sciences, Hohhot 010031, China

Received date: 2013-12-05

  Online published: 2014-05-30

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摘要

本试验旨在研究乙酸钠和β-羟丁酸钠对奶牛乳腺上皮细胞（BMECs）乳脂和乳蛋白合成相关基因表达的影响。试验分2部分，均采用单因子完全随机试验设计。第1部分，单独添加试验，乙酸钠的添加浓度分别为0（对照）、6.00、9.00、12.00和15.00 mmol/L，β-羟丁酸钠的添加浓度分别为0（对照）、0.80、1.60和2.40 mmol/L。第2部分，混合添加试验，以单独添加试验得出的乙酸钠和β-羟丁酸钠的适宜浓度，二者之和为总添加浓度，设定3种不同配比，即乙酸钠：β-羟丁酸钠分别为1：1、2：1和4：1，对照组不添加乙酸钠和β-羟丁酸钠。结果表明：1）与对照组相比，12.00 mmol/L乙酸钠能够显著提高BMECs乙酰辅酶A羧化酶（ACC）、脂肪酸合成酶（FAS）、二酰甘油酰基转移酶（DGAT）、乙酰辅酶A合成酶2（ACSS2）、过氧化物酶体增殖物激活受体（PPARG）、κ酪蛋白（CSN3）和雷帕霉素靶蛋白（mTOR）基因的表达量及甘油三酯（TAG）的含量（P<0.05）。2）与对照组相比，2.40 mmol/L的β-羟丁酸钠能够显著提高BMECs ACC、FAS、ACSS2、PPARG、mTOR基因表达量及TAG含量（P<0.05）。3）添加不同配比的乙酸钠和β-羟丁酸钠均不同程度地促进了乳脂合成相关基因的表达，乙酸钠：β-羟丁酸钠为2：1和4：1时，BMECs中TAG含量显著高于为1：1时和对照组（P<0.05）。综合各项指标，以9.60 mmol/L乙酸钠和4.80 mmol/L β-羟丁酸钠混合添加对奶牛乳腺上皮细胞乳脂和乳蛋白合成的促进效果较好。

关键词： 奶牛乳腺上皮细胞; 乙酸钠; β-羟丁酸钠; 乳脂; 乳蛋白

本文引用格式

塔娜 , 李红磊 , 侯先志 , 考桂兰 , 高民 , 李大彪 . 乙酸钠和β-羟丁酸钠对奶牛乳腺上皮细胞乳脂和乳蛋白合成相关基因表达的影响[J]. 动物营养学报, 2014 , 26(6) : 1527 -1534 . DOI: 10.3969/j.issn.1006-267x.2014.06.013

Abstract

The aim of this study was to determine the effects of sodium acetate and sodium β-hydroxybutyrate on expressions of genes involved in milk fat and protein synthesis in bovine mammary epithelial cells (BMECs). The study was consisted of two parts, and completely random single-factor designs were adopted. Part 1: single supplementation test, the supplemental concentrations of sodium acetate and sodium β-hydroxybutyrate were 0 (control), 6.00, 9.00, 12.00 and 15.00 mmol/L, and 0 (control), 0.80, 1.60 and 2.40 mmol/L, respectively. Part 2: mixed supplementation test, based on data from part 1, the total supplemental concentration was the sum of optimal supplemental concentrations of sodium acetate and sodium β-hydroxybutyrate; three ratios of sodium acetate to sodium β-hydroxybutyrate (1:1, 2:1 and 4:1) were designed, and the treatment of no supplementation was used as a control group. The results showed as follows: 1) compared with control group, 12.00 mmol/L sodium acetate significantly increased the expression levels of acetyl-CoA carboxylase (ACC), fatty acid synthetase (FAS), diacylgycerol acyltransferase (DGAT), acetyl-CoA synthetase 2 (ACSS2), peroxisome prolifertor receptor (PPARG), κ-casein (CSN3) genes and the content of triglyceride (TAG) in BMECs (P<0.05). 2) Compared with control group, 2.40 mmol/L sodium β-hydroxybutyrate significantly increased the expression levels of ACC, FAS, ACSS2, PPARG and mammalian target of rapamycin (mTOR) genes and the content of TAG in BMECs (P<0.05). 3) When the ratios of sodium acetate to sodium β-hydroxybutyrate were 2:1 and 4:1, The supplementation of sodium acetate and sodium β-hydroxybutyrate at different mixed ratios significantly up-regulated the expression levels of genes involved in milk fat synthesis at different extents. The content of TAG in BMECs were significantly increased compared with when the ratio was 1:1 and control group (P<0.05). In conclusion, the mixed supplementation of 9.60 mmol/L sodium acetate and 4.80 mmol/L sodium β-hydroxybutyrate are optimal, considering its simulative effects on milk fat and protein synthesis.

Key words： bovine mammary epithelial cells; sodium acetate; sodium β-hydroxybutyrate; milk fat; milk protein

参考文献

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