本试验旨在探讨仿生消化法测定鸡饲料酶水解物能值（EHGE）的变异程度，为确定该方法的测试精度与变异因素提供参考。采用单因素完全随机设计，从3套同型号的单胃动物仿生消化系统（SDS-2）中随机选择仪器测定玉米、菜籽粕、棉籽粕的干物质消化率（DMD）和EHGE。每个样品分3个批次重复测定，每个批次设5个重复，每个重复1根消化管。分析仿生消化过程中各变量的变异系数及其与DMD和EHGE的相关性。结果表明：1）玉米、小麦、棉籽粕的DMD和EHGE的批内、批间与总变异系数均不超过1.00%。菜籽粕DMD的批内、批间与总变异系数均不超过1.00%，但EHGE的批内、批间与总变异系数最大值达到1.64%。仿生消化中，上样量、残渣的干物质量、残渣总能量、残渣能量浓度的批内、批间及总变异系数均分别低于0.40%、4.16%、4.17%和2.08%。2）玉米、菜籽粕的干物质上样量与DMD无显著性相关关系（P>0.05），仅棉籽粕的干物质上样量与DMD存在低度相关性（|r|0.05），而残渣的干物质量与DMD，残渣总能量与EHGE高度相关（|r|>0.95，P0.05）。由此可见，基于SDS-2的仿生消化法在测定鸡饲料的DMD和EHGE时具有满意的精度。残渣的干物质量的变异是影响DMD与EHGE测值变异的主要因素。

Variation of the method for determining the enzyme hydrolysate gross energy (EHGE) of feedstuff using in vitro bionic digestion was investigated to confirm the precision of assay and evaluate the factors contributed to the variation of determined values. A single factorial completely randomized design was adapted. Three computer-controlled simulated digestion system (SDS-2) were randomly selected to determine the dry matter digestibility (DMD) and EHGE in corn, wheat, rapeseed meal and cottonseed meal. Each feedstuff was repeatedly determined for the DMD and EHGE in 3 batches. Each batch contained 5 replicates with 1 digestion tube per replicate. The variation coefficients of variables for determining the DMD and EHGE were calculated and the correlation between these variables and DMD or EHGE were developed. The results showed as follows: 1) the intra-batch, inter-batch and total coefficient of variation (CV) were all less than 1.00% for DMD and EHGE in corn, wheat and cottonseed meal. The intra-batch, inter-batch and total CV were all less than 1.00% for DMD and 1.64% for EHGE in rapeseed meal, respectively. In bionic digestion, the intra-batch, inter-batch and total CV were all less than 0.40%, 4.16%, 4.17% and 2.08% for sample weight, residues weight, residues energy and residues energy content, respectively. 2) Correlation between sample weight and DMD was not significant for corn, wheat and rapeseed meal (P>0.05), also low for cottonseed meal (|r|0.05). However, high correlation between residues weight and DMD, and between residues energy and EHGE were observed for all feedstuffs (|r|>0.95, P0.05). In conclusion, the precision is acceptable for the method to determine DMD and EHGE of feedstuffs using bionic digestion based on SDS-2. The variation of residues weight is the main factor contributed to the variation of DMD and EHGE.