表达猪乳铁蛋白的重组乳酸乳球菌对断奶仔猪应用效果的研究

何佳; 黄薇薇; 孟庆森; 姜艳平; 崔文; 乔薪瑗; 葛俊伟; 刘敏; 李一经; 唐丽杰

doi:10.3969/j.issn.1006-267x.2014.06.019

动物营养学报 >

2014 , Vol. 26 >Issue 6: 1579 - 1586

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.06.019

饲料营养 Feed Science and Technology

表达猪乳铁蛋白的重组乳酸乳球菌对断奶仔猪应用效果的研究

何佳 ,
黄薇薇 ,
孟庆森 ,
姜艳平 ,
崔文 ,
乔薪瑗 ,
葛俊伟 ,
刘敏 ,
李一经 ,
唐丽杰

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东北农业大学动物医学学院, 哈尔滨 150030

收稿日期: 2013-12-18

网络出版日期: 2014-05-30

基金资助

国家自然科学基金项目（30172057、30178709）；黑龙江省高校科技创新团队项目（2011TD001）

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Application Effects of Recombinant Lactococcus lactis Expressing Porcine Lactoferrin of Weaner Piglets

HE Jia ,
HUANG Weiwei ,
MENG Qingsen ,
JIANG Yanping ,
CUI Wen ,
QIAO Xinyuan ,
GE Junwei ,
LIU Min ,
LI Yijing ,
TANG Lijie

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College of Veterinary Medicine, Northeast Agriculture University, Harbin 150030, China

Received date: 2013-12-18

Online published: 2014-05-30

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摘要

本试验以表达猪乳铁蛋白的重组乳酸乳球菌饲喂断奶仔猪，研究其作为生态友好安全型的饲料添加剂替代抗生素的可行性。试验选取体重相近的36头断奶仔猪，分为3组，每组3个重复，每个重复4头。对照组饲喂基础饲粮，抗生素组饲喂添加20 mg/kg黄霉素和110 mg/kg金霉素的基础饲粮，试验组在饲喂基础饲粮的基础上每日按仔猪体重添加1次表达猪乳铁蛋白的重组乳酸乳球菌发酵菌液，每头仔猪每天饲喂乳铁蛋白含量约为50 μg/kg。试验期21 d。结果表明：在饲粮中添加表达猪乳铁蛋白的重组乳酸乳球菌，与对照组相比，平均日增重显著提高（P0.05）；血清中白细胞介素-1含量显著高于对照组和抗生素组（P<0.05），血清中白细胞介素-2含量极显著高于对照组（P<0.01），显著高于抗生素组（P<0.05）。由此得出，在断奶仔猪饲粮中添加表达猪乳铁蛋白的重组乳酸乳球菌可促进仔猪肠道黏膜绒毛的发育，提高仔猪的饲料利用率，降低料重比，同时增强仔猪免疫功能，具有提高仔猪生长性能的作用。

关键词： 猪乳铁蛋白; 乳酸乳球菌; 断奶仔猪; 替代抗生素

本文引用格式

何佳 , 黄薇薇 , 孟庆森 , 姜艳平 , 崔文 , 乔薪瑗 , 葛俊伟 , 刘敏 , 李一经 , 唐丽杰 . 表达猪乳铁蛋白的重组乳酸乳球菌对断奶仔猪应用效果的研究[J]. 动物营养学报, 2014 , 26(6) : 1579 -1586 . DOI: 10.3969/j.issn.1006-267x.2014.06.019

Abstract

This experiment was conducted to investigate the feasibility of recombinant Lactococcus lactis expressing porcine lactoferrin as an ecological friendly secure additive to substitute antibiotics feeding weaner piglets. A total of 36 weaner piglets with similar body weight were selected and randomly divided into 3 groups with 3 replicates in each group and 4 piglets in each replicate. The control group was fed a basal diet, the antibiotics group was fed the basal diet added 20 mg/kg flavomycin and 110 mg/kg chlortetracycline, while the trial group was fed the basal diet added recombinant Lactococcus lactis fermentation liquid (supplement according to the body weight of piglets, and the contain of porcine lactoferrin was 50 μg/kg per piglet per day) once a day. The experiment lasted for 21 days. The results showed that adding the recombinant Lactococcus lactis expressing porcine lactoferrin in the piglet diet, average daily gain was significantly higher than that in control group (P0.05); the content of interleukin-1 in serum was significantly higher than that in control group and antibiotics group (P<0.05), the content of interleukin-2 in serum was significantly higher than that in control group (P<0.01), and significantly higher than that in antibiotics group (P<0.05). Adding recombinant Lactococcus lactis expressing porcine lactoferrin in weaner piglet diets can promote the growth of piglet intestinal mucosal villus, improve piglet feed utilization rate, decrease the feed/gain, at the same time, strengthen the piglet immune function, and has the effect of improving piglet growth performance.

Key words： porcine lactoferrin; Lactococcus lactis; weaner piglets; instead of antibiotics

参考文献

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