猪源性PR39抗菌肽对断奶仔猪生产性能、血清免疫指标和肠道菌群的影响

卢俊鑫; 杨金波; 邝哲师; 赵祥杰; 叶明强; 潘木水

doi:10.3969/j.issn.1006-267x.2014.06.020

动物营养学报 >

2014 , Vol. 26 >Issue 6: 1587 - 1592

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.06.020

饲料营养 Feed Science and Technology

猪源性PR39抗菌肽对断奶仔猪生产性能、血清免疫指标和肠道菌群的影响

卢俊鑫 ,
杨金波 ,
邝哲师 ,
赵祥杰 ,
叶明强 ,
潘木水

展开

1. 广东省农业科学院蚕业与农产品加工研究所, 广州 510610;
2. 华南农业大学动物科学学院, 广州 510642

收稿日期: 2013-12-23

网络出版日期: 2014-05-30

基金资助

广东省教育部产学研结合项目（2011B090300020）；广东省科技星火计划（2012A020602095）；国家星火计划项目（2013GA780059）

收起

Effects of Antimicrobial Peptide PR39 on Performance, Serum Immune Indexes and Intestinal Microflora of Weaner Piglets

LU Junxin ,
YANG Jinbo ,
KUANG Zheshi ,
ZHAO Xiangjie ,
YE Mingqiang ,
PAN Mushui

Expand

1. Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510610, China;
2. College of Animal Science, South China Agricultural University, Guangzhou 510642, China

Received date: 2013-12-23

Online published: 2014-05-30

Fold

摘要

本试验旨在研究饲粮中添加猪源性PR39抗菌肽对断奶仔猪生产性能、血清免疫指标和肠道菌群的影响。选用体重相近的28日龄断奶仔猪120头，随机分为4组，每组3个重复，每个重复10头（公母各占1/2）。对照组饲喂基础饲粮，试验Ⅰ组饲喂基础饲粮+120 mg/kg吉他霉素，试验Ⅱ组饲喂基础饲粮+200 mg/kg天蚕素抗菌肽，试验Ⅲ组饲喂基础饲粮+300 mg/kg PR39抗菌肽。试验期21 d。结果表明：与对照组相比，2种抗菌肽均显著提高了仔猪平均日增重（P0.05）。与对照组相比，2种抗菌肽均显著降低了盲肠、直肠、结肠中大肠杆菌数（P<0.05），试验Ⅱ组显著提高了盲肠双歧杆菌数（P<0.05）。由此可见，饲粮中添加PR39抗菌肽和天蚕素抗菌肽可改善断奶仔猪生产性能，降低腹泻率，提高血清免疫球蛋白含量，促进肠道有益菌增殖，抑制有害菌增殖。

关键词： 抗菌肽; 断奶仔猪; 生产性能; 血清免疫指标; 肠道菌群

本文引用格式

卢俊鑫 , 杨金波 , 邝哲师 , 赵祥杰 , 叶明强 , 潘木水 . 猪源性PR39抗菌肽对断奶仔猪生产性能、血清免疫指标和肠道菌群的影响[J]. 动物营养学报, 2014 , 26(6) : 1587 -1592 . DOI: 10.3969/j.issn.1006-267x.2014.06.020

Abstract

This experiment was conducted to investigate the effects of antimicrobial peptide PR39 on performance, serum immune indexes and intestinal microflora of weaner piglets. A total of 120 weaner piglets at 28 days of age with the similar body weight were randomly allotted into 4 groups with 3 replicates per group and 10 piglets per replicate (half male and half female). The piglets in control group were fed a basal diet, those in experimental group Ⅰ were fed the basal diet+120 mg/kg kitasamycin, those in experimental group Ⅱ were fed the basal diet+200 mg/kg ntimicrobial peptide cecropin, and those in experimental group Ⅲ were fed the basal diet+300 mg/kg antimicrobial peptide PR39. The experiment lasted for 21 days. The results showed as follows: compared with control group, the two kinds of antimicrobial peptides both significantly increased average daily gain of piglets, while significantly reduced feed to gain ratio and diarrhea rate (P0.05). Compared with control group, the two kinds of antimicrobial peptides both significantly reduced the number of Escherichia coli in cecum, colon and rectum (P<0.05), while the number of Bifidobacterium in cecum in experimental group Ⅱ was significantly increased (P<0.05). In conclusion, the dietary supplementation of antimicrobial peptides can improve performance of weaner piglets, decrease the diarrhea rate, increase serum immunoglobulin contents, and stimulate beneficial bacteria proliferation, but inhibit pathogenic bacteria proliferation.

Key words： antimicrobial peptides; weaner piglets; performance; serum immune indexes; intestinal microflora

参考文献

[1] YEAMAN M R,YOUNT N Y.Mechanisms of antimicrobial peptide action and resistance[J].Pharmacological Reviews,2003,55(1):27-55.

[2] 宋青龙,王洪彬,张广民,等.日粮中添加天蚕素抗菌肽对仔猪健康及生产性能的影响[J].中国畜牧杂志,2012,48(13):52-55.
[3] 王菊芳.防御素抗菌肽对断奶仔猪生产性能的影响试验[J].湖南畜牧兽医,2012(3):9-10.
[4] 罗治华,周洪波,苏向东,等.抗菌肽饲料添加剂对断奶仔猪生产性能的影响[J].当代畜牧,2009(7):23-24.
[5] 陈香,李波,易学武,等.添加天蚕素抗菌肽对断奶仔猪生长性能的影响[J].中国饲料,2011(12):25-30.
[6] 刘扬科,李希,杨利,等.日粮中添加天蚕素抗菌肽对降低断奶仔猪饲用抗生素使用量的影响[J].饲料与畜牧,2012(8):56-58.
[7] 李永新,黄木家.抗菌肽制剂对仔猪生长性能和健康程度的影响[J].今日养猪业,2010(11):43-44.
[8] 谢红兵.复方中草药免疫增强剂对断奶仔猪免疫功能的影响[D].硕士学位论文.福州:福建农林大学,2004:30-31.
[9] TANG Z R,YIN Y L,ZHANG Y M,et al.Effects of dietary supplementation with an expressed fusion peptide bovine lactoferricin-lactoferrampin on performance,immune function and intestinal mucosal morpholopy in piglets weaned at age 21 d[J].British Journal of Nutrtion,2009,101(7):998-1005.

[10] 王阿荣,敖长金,宋青龙,等.日粮中添加天蚕素抗菌肽对断奶仔猪生产性能和血液生化指标的影响[J].饲料工业,2011,32(10):21-24.
[11] BAO H,SHE R,LIU T,et al.Effects of pig antibacterial peptides on growth performance and intestine mucosal immune of broiler chickens[J].Poultry Science,2009,88(2):291-297.

[12] 郭志强,杨奉珠,雷岷,等.抗菌肽对肉兔小肠黏膜形态、盲肠菌群和免疫功能的影响[J].动物营养学报,2012,24(9):1778-1784.
[13] WANG D,MA W,SHE R,et al.Effects of swine gut antimicrobial peptides on the intestinal mucosal immunity in specific-pathogen-free chickens[J].Poultry Science,2009,88(5):967-974.

[14] RADDYK V R,YEDERY R D,ARANHA C.Antimicrobial peptides:premises and promises[J].International Journal of Antimicrobial Agents,2004,24(6):536-547.

[15] CHEN X,ZHU F,CAO Y,et al.Novel expression vector for secretion of cecropin AD in Bacillus subtilis with enhanced antimicrobial activity[J].Antimicrobial Agents and Chemotherapy,2009,53(9):3683-3689.

[16] 张董燕,季海峰,刘辉,等.天蚕素抗菌肽对断奶仔猪生产性能及血清指标的影响[J].饲料研究,2011(10):22-24.
[17] 都明海,戚广州,王建军,等.抗菌脂肽对断奶仔猪生长性能、肠道微生物及血液指标的影响研究[J].中国粮油学报,2011,26(5):76-82.

Options

文章导航

摘要

本文引用格式

Abstract

参考文献

关于本刊

在线期刊

作者中心

相关单位

联系我们

模态框（Modal）标题

摘要

本文引用格式

Abstract

参考文献

关于本刊

在线期刊

作者中心

相关单位

联系我们