饲料营养 Feed Science and Technology

玉米-杂粕型饲粮中添加植酸酶对蛋鸡生产性能、钙磷代谢及肠道形态的影响

  • 李连彬 ,
  • 陈秀丽 ,
  • 岳洪源 ,
  • 高玉鹏 ,
  • 武书庚
展开
  • 1. 西北农林科技大学动物科技学院, 杨凌 712100;
    2. 中国农业科学院饲料研究所, 农业部饲料生物技术重点开放实验室, 北京 100081

收稿日期: 2013-12-23

  网络出版日期: 2014-05-30

基金资助

现代农业产业技术体系(CARS-41-K13);现代农业产业技术体系北京市家禽创新团队(CARS-PSTP)

Effects of Phytase on Performance, Metabolism of Calcium and Phosphorus and Intestinal Morphology of Layer Hens Fed Corn-Miscellaneous Meal Diets

  • LI Lianbin ,
  • CHEN Xiuli ,
  • YUE Hongyuan ,
  • GAO Yupeng ,
  • WU Shugeng
Expand
  • 1. College of Animal Science and Technology, Northwest A & F University, Yangling 712100, China;
    2. Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received date: 2013-12-23

  Online published: 2014-05-30

摘要

本试验旨在研究玉米-杂粕型饲粮中植酸酶添加水平对蛋鸡生产性能、蛋品质、钙磷代谢及肠道形态的影响。选取600只产蛋率和体重相近、健康的24周龄海兰褐蛋鸡,随机分为5组,每组8个重复,每个重复15只鸡。正对照组(PC组)饲喂总磷为0.60%(有效磷为0.36%)的正常基础饲粮;负对照组(NC组)饲喂总磷为0.40%(有效磷为0.16%)的低磷的基础饲粮;其余3个试验组(P125、P250和P500组)在NC组饲粮基础上分别添加125、250和500 U/kg的植酸酶。试验期为24周。结果表明:1)与NC组比较,P250和P500组产蛋率、平均蛋重和平均日采食量显著提高(P0.05);饲粮中添加植酸酶对料蛋比无显著影响(P>0.05),但有降低的趋势,对蛋品质无显著影响(P>0.05);2)与NC组相比,饲粮中添加植酸酶对血浆钙和胫骨钙含量无显著影响(P>0.05),P250和P500组血浆磷、胫骨灰分和磷含量及磷表观消化率显著提高(P0.05);3)与NC组相比,P250和P500组隐窝深度显著降低(P<0.05),绒毛高度/隐窝深度显著升高(P<0.05)。结果提示,低磷玉米-杂粕型饲粮中添加植酸酶能够改善蛋鸡生产性能、钙磷代谢及肠道的发育;本试验条件下,饲粮中添加250 U/kg的植酸酶较为适宜。

本文引用格式

李连彬 , 陈秀丽 , 岳洪源 , 高玉鹏 , 武书庚 . 玉米-杂粕型饲粮中添加植酸酶对蛋鸡生产性能、钙磷代谢及肠道形态的影响[J]. 动物营养学报, 2014 , 26(6) : 1593 -1600 . DOI: 10.3969/j.issn.1006-267x.2014.06.021

Abstract

This experiment was conducted to study the effects of phytase supplemental level on performance, egg quality, metabolism of calcium and phosphorus and intestinal morphology of laying hens fed corn-miscellaneous meal diets. Six hundred Hy-Line Brown hens were randomly divided into 5 groups with 8 replicates per group and 15 laying hens per replicate. Positive control (PC) group was fed a basal diet containing 0.60% total phosphorus (0.36% available phosphorus), negative control (NC) group was fed the basal diet containing 0.40% total phosphorus (0.16% available phosphorus), and three experimental groups (P125 group, P250 group and P500 group) were fed the NC group diets supplemented with 125, 250, and 500 U/kg phytase, respectively. The experiment lasted for 24 weeks. The results showed as follows: 1) compared with NC group, laying rate, average egg weight and average daily feed intake in P250 group and P500 group were significantly increased (P0.05); there were no effects of phytase on feed/egg and egg quality (P>0.05). 2) Compared with NC group, tibia calcium and plasma calcium contents were not affected by dietary phytase (P>0.05), plasma phosphorus and tibia ash and phosphorus contents, and the apparent digestibility of phosphorus in P250 group and P500 group were significantly higher than those in NC group (P0.05). 3) Compared with NC group, the crypt depth in P250 group and P500 group was significantly decreased (P<0.05) and the villous height/crypt depth was significantly increased (P<0.05). In conclusion, the supplementation of phytase in corn-miscellaneous meal diets can improve performance, calcium and phosphorus digestibility and intestinal development of laying hens; the optimal level of phytase is 250 U/kg in the present study.

参考文献

[1] 李爱科,郝淑红,张晓琳,等.我国饲料资源开发现状及前景展望[J].畜牧市场,2007(9):13-16.
[2] 邓敏.杂粕型日粮添加复合酶对丽佳鸭生产性能和消化功能的影响[D].硕士学位论文.福州:福建农林大学,2010.
[3] ZAGHARI M,GAYKANI R,SHIVAZAD M,et al.Evaluation of phytase nutrient equivalency for old layer hens[J].Asian Journal of Poultry Science,2008,2:24-29.
[4] HUGHES A L,DAHIYA J P,WYATT C L,et al.The efficacy of quantum phytase in a forty-week production trial using white leghorn laying hens fed corn-soybean meal-based diets[J].Poultry Science,2008,87(6):1156-1161.  
[5] SKŘIVAN M,ENGLMAIEROVÁ M,SKŘIVANOVÁ V.Effect of different phosphorus levels on the performance and egg quality of laying hens fed wheat-and maize-based diets[J].Czech Journal of Animal Science,2010,55:420-427.
[6] GORDON R W,ROLAND D A.Performance of commercial laying hens fed various phosphorus levels,with and without supplemental phytase[J].Poultry Science,1997,76(8):1172-1177.  
[7] SIMONS P C M,VERSTEEGH H A L,JONGBLOED A W,et al.Improvement of phosphorus availability by microbial phytase in broilers and pigs[J].British Journal of Nutrition,2007,64(2):525-540.
[8] 雷乔波,石凌霄,张克英,等.不同剂型植酸酶对蛋鸡生产性能、蛋品质和骨骼质量的影响[J].动物营养学报,2010,22(5):1374-1381.
[9] ZYLA K,MIKA M,DULI N ' SKI R,et al.Effects of inositol,inositol-generating phytase B applied alone,and in combination with 6-phytase A to phosphorus-deficient diets on laying performance,eggshell quality,yolk cholesterol,and fatty acid deposition in laying hens[J].Poultry Science,2012,91(8):1915-1927.  
[10] JALAL M A,SCHEIDELER S E.Effect of supplementation of two different sources of phytase on egg production parameters in laying hens and nutrient digestibility[J].Poultry Science,2001,80(10):1463-1471.  
[11] MUSTAFA S,AHMET G,MEHMET D.Egg production and calcium-phosphorus utilization of laying hens fed diets supplemented with phytase alone or in combination with organic acid[J].International Journal of Poultry Science,2012,11(3):181-189.  
[12] 方热军,贺佳,曹满湖,等.日粮磷水平对肉鸡磷代谢及Na/Pi-Ⅱb基因mRNA表达的影响[J].畜牧兽医学报,2011,42(2):289-296.
[13] COLLAZOS H,GRANADOS J,PIERNAGORDA D.Phytase supplementation in cereals by-products based diets and effects on production,eggshell quality,calcium and phosphorus in laying hens [C]//ⅩⅫ world’s poultry congress,Istanbul: [s.n.],2004:395.
[14] KESHAVARZ K.The effect of different levels of nonphytate phosphorus with and without phytase on the performance of four strains of laying hens[J].Poultry Science,2003,82(1):71-91.  
[15] LIM H S,NAMKUNG H,PAIK I K.Effects of phytase supplementation on the performance,egg quality and phosphorous excretion of laying hens fed different levels of dietary calcium and nonphytate phosphorous[J].Poultry Science,2003,82(1):92-99.  
[16] LIEBERT F,HTOO J K,SUNDER A.Performance and nutrient utilization of laying hens fed low-phosphorus corn-soybean and wheat-soybean diets supplemented with microbial phytase[J].Poultry Science,2005,84:1576-1583.
[17] HUGHES A L,DAHIYA J P,WYATT C L,et al.Effect of quantum phytase on nutrient digestibility and bone ash in white leghorn laying hens fed corn-soybean meal-based diets[J].Poultry Science,2009,88(6):1191-1198.  
[18] 成令忠.组织学[M].2版.北京:人民卫生出版社,1994.
[19] 邱梅平,王恬.低磷日粮中添加植酸酶对蛋鸡肠道形态结构的影响[J]饲料工业,2009,30(24):20-23.
[20] SELLE P H,RAVINDRAN V.Microbial phytase in poultry nutrition[J].Animal Feed Science and Technology,2007,135(1/2):1-41.
文章导航

/