益生菌和黄曲霉毒素B1降解酶对肉仔鸡生长性能的影响及其作用机理

程伟; 左瑞雨; 常娟; 尹清强; 王平; 党晓伟

doi:10.3969/j.issn.1006-267x.2014.06.023

动物营养学报 >

2014 , Vol. 26 >Issue 6: 1608 - 1615

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.06.023

饲料安全 Feed Security

益生菌和黄曲霉毒素B₁降解酶对肉仔鸡生长性能的影响及其作用机理

程伟 ,
左瑞雨 ,
常娟 ,
尹清强 ,
王平 ,
党晓伟

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1. 河南农业大学牧医工程学院, 郑州 450002;
2. 河南牧业经济学院, 郑州 450011;
3. 河南省畜牧局, 郑州 450008;
4. 河南德邻生物制品有限公司, 新乡 453000

收稿日期: 2013-12-01

网络出版日期: 2014-05-30

基金资助

郑州市科技创新团队（112PCXTD339）

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Effect of Probiotics and Aflatoxin B₁ Degrading Enzyme on Growth Performance of Broilers and Its Mechanisms

CHENG Wei ,
ZUO Ruiyu ,
CHANG Juan ,
YIN Qingqiang ,
WANG Ping ,
DANG Xiaowei

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1. College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China;
2. Henan College of Animal Husbandry and Economics, Zhengzhou 450011, China;
3. Henan Animal Husbandry Bureau, Zhengzhou 450008, China;
4. Henan Delin Biological Product Co., Ltd., Xinxiang 453000, China

Received date: 2013-12-01

Online published: 2014-05-30

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摘要

本试验旨在通过益生菌与黄曲霉毒素B₁（AFB₁）降解酶的高效组合制备霉菌毒素解毒剂，测定其对肉仔鸡生长性能的影响。试验选择1日龄健康爱拔益加（AA）肉仔鸡200只，随机分为5个组，每组5个重复，每个重复8只鸡。A组（对照组）饲喂基础饲粮，B（负对照组）、C、D和E组分别在基础饲粮中添加100 μg/kg的AFB₁，并按照0、0.05%、0.10%和0.15%的比例分别添加益生菌和AFB₁降解酶。试验期21 d。结果表明：与负对照组相比，添加益生菌和AFB₁降解酶可不同程度地提高肉鸡的平均日增重、营养物质代谢率及盲肠中乳酸菌的数量，显著降低肉鸡的死亡率、腹泻率及十二指肠中大肠杆菌数量（P<0.05）。添加0.15%益生菌和AFB₁降解酶可使血清中低密度脂蛋白含量降低（P<0.05），而间接胆红素含量、谷酰转肽酶活性和总抗氧化能力显著升高（P<0.05）。由此可见，益生菌与AFB₁降解酶的组合，可降低采食高剂量AFB₁对肉仔鸡生长性能的不良影响。

关键词： 黄曲霉毒素B₁; 益生菌; 解毒酶; 肉仔鸡; 生长性能

本文引用格式

程伟 , 左瑞雨 , 常娟 , 尹清强 , 王平 , 党晓伟 . 益生菌和黄曲霉毒素B₁降解酶对肉仔鸡生长性能的影响及其作用机理[J]. 动物营养学报, 2014 , 26(6) : 1608 -1615 . DOI: 10.3969/j.issn.1006-267x.2014.06.023

Abstract

Probiotics and aflatoxin B₁ (AFB₁) degrading enzyme were combined to make detoxificating materials for determining its effects on growth performance of broilers and its mechanisms. A total of 200 one-day-old Arbor Acres (AA) broilers were randomly allocated to 5 groups with 5 replicates per group and 8 broilers per replicate. Broilers in group A (control group) were fed a basal diet, broilers in groups B (negative control group), C, D and E were fed the basal diet containing 100 μg/kg AFBl, and supplemented with 0, 0.05%, 0.10% and 0.15% probiotics and AFB₁ degrading enzyme, respectively. The experiment lasted for 21 days. The results showed that compared with group B, the average daily gain, nutrient metabolic rates and caecum lactic acid bacteria number were increased, and mortality rate and diarrhea rate were significantly decreased in the groups added with probiotics and AFB₁ degrading enzyme (P<0.05). Adding 0.15% probiotics and AFB₁ degrading enzyme could decrease the serum low-density lipoprotein content, while increase the indirect bilirubin content, glutamyltransferase activity and total anti-oxidation ability (P<0.05). It is concluded that the combination of probiotics and AFB₁-degrading enzyme can decrease the negative effect of high dietary AFB₁ concentration on growth performance of broilers.

Key words： aflatoxin B₁; probiotics; detoxificating enzyme; broilers; growth performance

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