博落回生物碱对猪肠上皮细胞增殖的影响

李杰; 伍树松; 熊兴耀; 苏丁丁; 贺建华

doi:10.3969/j.issn.1006-267x.2014.06.026

动物营养学报 >

2014 , Vol. 26 >Issue 6: 1632 - 1637

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.06.026

研究简报 Short Communications

博落回生物碱对猪肠上皮细胞增殖的影响

李杰 ,
伍树松 ,
熊兴耀 ,
苏丁丁 ,
贺建华

展开

1. 湖南农业大学食品科学技术学院, 长沙 410128;
2. 湖南农业大学动物科学技术学院, 长沙 410128;
3. 作物种质创新与资源利用国家重点实验室培育基地, 长沙 410128

收稿日期: 2013-12-13

网络出版日期: 2014-05-30

基金资助

湖南省科技重大专项（2012FJ1004-2）；湖南省自然科学基金省市联合重点项目（12JJ8014）；国家自然科学基金青年项目（31302005）

收起

Effects of Macleaya cordata Alkaloids on Intestinal Porcine Epithelial Cells Proliferation

LI Jie ,
WU Shusong ,
XIONG Xingyao ,
SU Dingding ,
HE Jianhua

Expand

1. College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China;
2. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;
3. Hunan Provincial Key Laboratory for Germplasm Innovation and Utilization of Crop, Changsha 410128, China

Received date: 2013-12-13

Online published: 2014-05-30

Fold

摘要

本试验旨在研究博落回中血根碱、白屈菜红碱、原阿片碱和别隐品碱在不同浓度、不同时间段对猪肠上皮细胞（IPEC-1）增殖的影响。IPEC-1预培养24 h后，在培养基中分别加入不同浓度（4、2、1、0.5、0.25、0.1、0.05、0.025、0.012 5、0.006 25 μg/mL的血根碱或白屈菜红碱；100、50、25、12.5、6.25、3.125、1.562 5 μg/mL的原阿片碱或别隐品碱，各组不同浓度梯度均设8个重复）的博落回生物碱继续培养12或24 h，4-甲基偶氮唑盐（MTT）染色法检测细胞增殖。结果表明：0.25~4 μg/mL的血根碱、0.25~4 μg/mL的白屈菜红碱、100 μg/mL的原阿片碱或100 μg/mL的别隐品碱对IPEC-1的增殖有显著的抑制作用（P0.05）。由此可见，博落回生物碱在一定浓度范围内可促进IPEC-1的增殖。这说明博落回生物碱提高动物生长性能可能是通过促进小肠细胞增殖分化更新，从而增强小肠消化、吸收、免疫屏障和应激反应等功能的途径实现的。

关键词： 博落回生物碱; IPEC-1; MTT; 增殖

本文引用格式

李杰 , 伍树松 , 熊兴耀 , 苏丁丁 , 贺建华 . 博落回生物碱对猪肠上皮细胞增殖的影响[J]. 动物营养学报, 2014 , 26(6) : 1632 -1637 . DOI: 10.3969/j.issn.1006-267x.2014.06.026

Abstract

To study the effects of sanguinarine, chelerythrine, protopine and allocryptopine from Macleaya cordata on intestinal porcine epithelial cells (IPEC-1) proliferation at different concentrations and different periods. IPEC-1 cells were pre-incubated for 24 hours, and then exposed to different concentrations (4, 2, 1, 0.5, 0.25, 0.1, 0.05, 0.025, 0.012 5 and 0.006 25 μg/mL of sanguinarine or chelerythrine, and 100, 50, 25, 12.5, 6.25, 3.125 and 1.562 5 μg/mL of protopine or allocryptopine; each treatment had 8 replicates) for additional 12 or 24 hours, respectively, cell viability was measured by MTT assay. The results showed as follows: after the IPEC-1 cells were treated by 0.25 to 4 μg/mL of sanguinarine, 0.25 to 4 μg/mL of chelerythrine, 100 μg/mL of protopine or 100 μg/mL of allocryptopine for 12 hours, the proliferation of cells was significantly inhibited (P0.05). The results indicate that the Macleaya cordata alkaloids can promote the proliferation of IPEC-1 under certain concentrations; it speculates that Macleaya cordata alkaloids may improve the intestinal digestive, absorptive, immune and barrier function through this way, and then improve the animal growth performance.

Key words： Macleaya cordata alkaloids; IPEC-1; MTT; proliferation

参考文献

[1] 林启寿.中草药成分化学[M].北京:科学出版社,1977:72.
[2] 谷千秋,高尾樽雄.博落回植物碱的研究[J].药学杂志(日),1962,82:755.
[3] KOSINA P,WALTEROVÁ D,ULRICHOVÁ J,et al.Sanguinarine and chelerythrine:assessment of safety on pigs in ninety days feeding experiment[J].Food and Chemical Toxicology,2004,42(1):85-91.
[4] 蔡鹏,孙志良,曾建国,等.不同剂量博落回提取物对断奶仔猪生长性能的影响[J].中国畜牧兽医,2010,37(5):41-43.
[5] 满意,张春勇,李美荃,等.博落回提取物对早期断奶仔猪生长性能和血清免疫参数的影响[J].动物营养学报,2013,25(1):126-132.
[6] VIEIRA S L,BERRES J,REIS R N,et al.Studies with sanguinarine like alkaloids as feed additive in broiler diets[J].Brazilian Journal of Poultry Science,2008,10(1):67-71.
[7] 许泷,陈湘滨,曾建国,等.博落回提取物(MCE)中主要生物碱与硫酸成盐研究[J].中兽医医药杂志,2011,13(6):20-23.
[8] 庞发根.博落回抗癌活性成分的研究[D].硕士学位论文.沈阳:沈阳药科大学,2005.
[9] 杨军,王静,刘信顺,等.博落回的药效研究[J].中药材,1999,22(2):82-85.
[10] 郭小清,唐莉苹,聂建超,等.博落回的药理作用及其在动物保健中的应用[J].中国动物保健,2005(5):34-35.
[11] LENFELD J,KROUTIL M,MARSÁLEK E,et al.Antiinflammatory activity of quaternary benzophenanthridine alkaloids from Chelidonium majus[J].Planta Medica,1981,43(2):161-165.
[12] BIANCO F,BASINI G,GRASSELLI F.The plant alkaloid sanguinarine affects swine granulosa cell activity[J].Reproductive Toxicology,2006,21(3):335-340.
[13] DRSATA J.Sanguinarine and chelerythrine as inhibitors of aromatic amino acid decarboxylase[J].Journal of Enzyme Inhibition and Medicinal Chenistry,1996,10(4):231-237.

[14] BOUHET S,HOURCADE E,LOISEAU N,et al.The mycotoxin fumonisin B₁ alters the proliferation and the barrier function of porcine intestinal epithelial cells[J].Toxicological Sciences,2004,77(1):165-171.
[15] YAMAJI S,TENNANT J,TANDY S,et al.Zinc regulates the function and expression of the iron transporters DMTl and IREGI in human intestinal Caco-2 cells[J].FEBS Letters,2001,507(2):137-141.

[16] SUKHOTNIK I,AGAM M,SHAMIR R,et al.Oral glutamine prevents gut mucosal injury and improves mucosal recovery following lipopolysaccharide endotoxemia in a rat [J].Journal of Surgical Research,2007,143(2):379-384.
[17] 郭小清,唐莉萍.中药博落回的研究进展[J].兽药市场指南,2006(5):11-12.

Options

文章导航

摘要

本文引用格式

Abstract

参考文献

关于本刊

在线期刊

作者中心

相关单位

联系我们

模态框（Modal）标题

摘要

本文引用格式

Abstract

参考文献

关于本刊

在线期刊

作者中心

相关单位

联系我们