动物营养学报 >

2014 , Vol. 26 >Issue 8: 2162 - 2168

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.08.018

反刍与草食动物营养 Ruminants and Herbivorous Animal Nutrition

甘氨酸对奶牛乳腺上皮细胞增殖与凋亡的影响

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  • 1. 内蒙古农业大学动物科学学院, 呼和浩特 010018;
    2. 内蒙古农牧业科学院动物营养研究所, 呼和浩特 010031
汤明惠（1988-），女，辽宁本溪人，硕士研究生，研究方向为动物营养与畜产品品质。E-mail：tangminghui126621@126.com

收稿日期: 2014-03-10

  网络出版日期: 2014-08-13

基金资助

国家“973”计划项目（2011CB100803）

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Effects of Glycine on Proliferation and Apoptosis of Mammary Epithelial Cells of Dairy Cows

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  • 1. College of Animal Science, Inner Mongolia Agricultural University, Huhhot 010018, China;
    2. Animal Nutrition Institute, Inner Mongolia Academy of Agriculture and Animal Husbandry Sciences, Huhhot 010031, China

Received date: 2014-03-10

  Online published: 2014-08-13

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摘要

本试验旨在研究甘氨酸（Gly）对体外培养的奶牛乳腺上皮细胞增殖与凋亡的影响。选用中国荷斯坦奶牛乳腺上皮细胞第2代细胞，分为6组，培养时在DMEM/F12培养基中分别添加0、1、2、5、10和20 mmol/L的Gly。每组6个重复，培养细胞24、48和72 h，检测细胞增殖；试验重复3次。每组4个重复，培养细胞24 h，测定细胞凋亡率和处于不同细胞周期的细胞比率。结果表明：与0 mmol/L组比较，1）1 mmol/L组细胞增殖能力略有提高，但差异不显著（P>0.05）；培养基中添加10~20 mmol/L Gly，细胞增殖能力显著降低（P<0.05）。2）培养基中添加2~20 mmol/L Gly显著提高了细胞凋亡率（P<0.05）；20 mmol/L组显著高于其余各组（P<0.05）。3）20 mmol/L组处于DNA合成期的细胞比率显著提高（P<0.05）；培养基中添加5~20 mmol/L Gly，DNA合成后期/细胞分裂期数值上略有升高，但差异不显著（P>0.05）。结果提示：Gly能够调节奶牛乳腺上皮细胞的增殖和凋亡，对细胞增殖具有低水平（1 mmol/L）促进，高水平（10~20 mmol/L）抑制的作用，对细胞凋亡有促进作用；Gly能够促进乳腺上皮细胞向DNA合成期转化，促进细胞分裂。

关键词： 甘氨酸; 乳腺上皮细胞; 细胞增殖; 细胞凋亡

本文引用格式

汤明惠, 张兴夫, 丹妮, 敖长金, 高民 . 甘氨酸对奶牛乳腺上皮细胞增殖与凋亡的影响[J]. 动物营养学报, 2014 , 26(8) : 2162 -2168 . DOI: 10.3969/j.issn.1006-267x.2014.08.018

Abstract

The objective of this experiment was to study the effects of glycine on proliferation and apoptosis of in vitro cultured mammary epithelial cells of dairy cows. The 2nd generation of mammary epithelial cells of Chinese Holstein dairy cows were used. Cells were divided into 6 groups, and were cultured in DMEM/F12 supplemented with 0, 1, 2, 5, 10 and 20 mmol/L glycine, respectively. There were 6 replicates in each group for the analysis of cell proliferation after cultured for 24, 48 and 72 hours (the test was repeated for 3 times), and there were 4 replicates in each group for the analysis of cell apoptosis rate and the proportion of cells in different cell cycles. The results showed as follows: compared with 0 mmol/L group, 1) the cell proliferation ability was improved in 1 mmol/L group, but the difference was not significant (P>0.05); the supplementation of 10 to 20 mmol/L glycine in culture medium significantly decreased the cell proliferation ability (P<0.05). 2) The supplementation of 2 to 20 mmol/L glycine in culture medium significantly increased the cell apoptosis rate (P<0.05), and the rate in 20 mmol/L group was significantly higher than that in the other groups (P<0.05). 3) The proportion of cells in S phase in 20 mmol/L group was significantly increased (P<0.05); the supplementation of 5 to 20 mmol/L glycine in culture medium tended to increase G2 phase/M phase, but the difference was not significant (P>0.05). In conclusion, glycine can regulate proliferation and apoptosis of mammary epithelial cells of dairy cows, has a low level (1 mmol/L) stimulative and high level (10 to 20 mmol/L) inhibitive effect on cell proliferation and a stimulative effect on cell apoptosis, and can promote the conversion of mammary epithelial cells to S phase and cell division.

Key words： glycine; mammary epithelial cell; cell proliferation; cell apoptosis

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