饲料营养 Feed Science and Technology

硒对黄曲霉毒素B1暴露肉鸡肝脏氧化损伤的保护作用

展开
  • 南京农业大学动物科技学院, 南京 210095
芮小丽(1988-),女,安徽马鞍山人,硕士研究生,动物营养与饲料科学专业。E-mail:ruixiaoli1988@163.com

收稿日期: 2014-03-03

  网络出版日期: 2014-08-13

基金资助

江苏省高等学校大学生实践创新训练计划(1310307018x);青蓝工程

Protective Effects of Selenium on aflatoxin B1 Induced Hepatic Oxidative Stress in Broilers

Expand
  • College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China

Received date: 2014-03-03

  Online published: 2014-08-13

摘要

本试验旨在研究硒(Se)对黄曲霉毒素B1(AFB1)毒性的缓解作用及其可能机制。选取1日龄爱拔益加(AA)肉仔鸡90只,随机分为3组(每组3个重复,每个重复10只):基础饲粮组(对照组)、基础饲粮+100 μg/kg AFB1组(AFB1组)、基础饲粮+100 μg/kg AFB1+0.3 mg/kg Se组(Se组),试验期为42 d。结果表明:1)与对照组相比,AFB1组肉鸡肝脏重显著降低(P<0.05);血清谷丙转氨酶活性显著升高(P<0.05);肝脏丙二醛和过氧化氢含量显著升高(P<0.05),总超氧化物歧化酶活性、谷胱甘肽含量和谷胱甘肽过氧物酶活性则显著降低(P<0.05);肝脏组织病理切片观察到多处出血位点及肝细胞坏死和萎缩;免疫组织化学和蛋白质印迹分析发现核转录因子E2相关因子2(Nrf2)蛋白在肝细胞核内表达显著增加(P<0.05),凋亡因子含半胱氨酸的天冬氨酸蛋白水解酶-3(caspase-3)在AFB1组胞核内表达。2)在100 μg/kg AFB1饲粮中加入Se能在一定程度上缓解AFB1引起的肉鸡肝功能紊乱和肝脏氧化应激损伤,并使肝细胞胞核Nrf2表达水平显著下降(P<0.05)。由此可见,Se能够缓解AFB1引起的肉鸡肝脏功能紊乱和氧化损伤,增加肉鸡抗氧化能力。

关键词: AFB1; ; 肝脏; 氧化应激; Nrf2; 肉鸡

本文引用格式

芮小丽, 陈思潭, 李春梅 . 硒对黄曲霉毒素B1暴露肉鸡肝脏氧化损伤的保护作用[J]. 动物营养学报, 2014 , 26(8) : 2281 -2288 . DOI: 10.3969/j.issn.1006-267x.2014.08.032

Abstract

To investigate the protective effects of selenium(Se) on aflatoxin B1 (AFB1) exposed broilers and its probable mechanism, ninety one-day-old broilers were randomly allotted to 3 groups (3 replicates per group and 10 birds per replicate): basal diet group (control group), basal diet+100 μg/kg AFB1 group (AFB1 group), basal diet 100 μg/kg AFB1 +0.3 mg/kg Se group (Se group). The trial lasted for 42 days. The results showed as follows: 1) compared with control group, the broilers in AFB1 group had a significantly lower liver weight (P<0.05), and serum glutamic pyruvic transaminase (GPT) activity was significantly increased (P<0.05). The contents of maleic dialdehyde (MDA) and H2O2 in AFB1 group were higher than those in control group (P<0.05), but total superoxide dismutase (T-SOD) activity, glutathione (GSH) content and glutathione peroxidase (GSH-Px) activity were significantly decreased (P<0.05). Multiple bleeding sites and hepatocyte necrosis and atrophy were observed by liver histopathology in AFB1 group. Moreover, the expression of NF-E2-related factor 2 (Nrf2) (P<0.05) and caspase-3 were increased in hepatocyte nucleus of AFB1 exposed broilers. 2) Compared with AFB1 group, the liver function indexes were improved by Se addition. Hepatic damage was reduced while Nrf2 expression in hepatocyte nucleus was decreased (P<0.05). These results suggest that Se could attenuate liver dysfunction and oxidative damage, improve performance, increase antioxidant ability in the AFB1 exposed broilers.

参考文献

[1] Council for Agricultural Science and Technology (CAST) of USA.Mycotoxins:economic and health risks[M].Ames, Iowa.Council for Agricultural Science and Technology, 1989, 116:91-91.

[2] SMILEY R D, DRAUGHON F A.Preliminary evidence that degradation of aflatoxin B1 by Flavobacterium aurantiacum is enzymatic[J].Journal of Food Protection, 2000, 63(3):415-418.

[3] LEESON S, DIAZ G, SUMMERS J D.Poultry metabolic disorders and mycotoxins[M].Britain:Nottingham University Press, 1995.

[4] TOWNER R A, QIAN S T, KADⅡSKA M B, et al.In vivo identification of afiatoxin-induced free radicals in rat bile[J].Free Radical Biology and Medicine, 2003, 35(10):1330-1340.  

[5] TOWNER R A, MASON R P, REINKE L A.In vivo detection of afiatoxin-induced lipid free radicals in rat bile[J].Biochimica et Biophysica Acta(BBA):General Subjects, 2002, 1573(1):55-62.  

[6] EATON D L, GROOPMAN J D.The toxicology of afiatoxins:human health, veterinary, and agricultural significance[M].London:Academic Press, 1994:544.

[7] ABDEL-WAHHAB M A, OMARA E A, ABDEL-GALIL M M, et al.Zizyphus spina-christi extract protects against afiatoxin B1-initiated hepatic carcinogenicity[J].African Journal of Traditional Complementary and Alternative Medicines, 2007, 4(3):248-256.

[8] ABDEL-WAHHAB M A, HASSAN N S, EI-KADY A A, et al.Red ginseng extract protects against afiatoxin B1 and fumonisins-induced hepatic pre-cancerous lesions in rats[J].Food and Chemical Toxicology, 2010, 48(2):733-742.  

[9] KWAK M K, WAKABAYASHI N, KENSLER T W.Chemoprevention through the Keap1-Nrf2 signaling pathway by phase 2 enzyme inducers[J].Mutation Research, 2004, 555(1):133-148.

[10] MIN K J, KIM J H, JOU L, et al.Adenosine induces hemeoxygenase-1 expression in microglia through the activation of phosphatidylinositol 3-kinase and nuclear factor E2-related factor 2[J].Glia, 2008, 56(9):1028-1037.  

[11] 钟敏.Nrf2-Keap1抗氧化系统研究进展[J].中国公共卫生, 2006, 22(3):360-362.

[12] 刘永旺, 刘海舰, 王宝琴, 等.富硒麦芽对链尿佐菌素诱发糖尿病大鼠糖脂代谢的影响[J].南京农业大学学报, 2004, 27(3):81-84.

[13] GREGORY Ⅲ J F.Effect of dietary selenium on the metabolism of aflatoxin B1 in turkeys[J].Food and Chemical Toxicology, 1984, 22(8):637-642.  

[14] GRIFFIN A C.Role of selenium in the chemoprevention of cancer[J].Advances in Cancer Research, 1979(29):419-442.

[15] 李红梅, 张勇.钠基膨润土和黄曲霉毒素吸附剂对肉鸡生长性能屠体性能及免疫性能的影响[J].当代畜牧, 2009(7):20-22.

[16] SHLIG A A.Effect of vitamin E and selenium supplement in reducing aflatoxicosis on performance and blood parameters in broiler chicks[J]. Iraqi Journal of Veterinary Sciences, 2009, 23:97-103.

[17] 卿中全, 于炎湖.黄曲霉毒素对家禽生产性能和健康的影响[J].中国饲料, 2000(3):35-36.

[18] 石达友, 廖申权, 刘念, 等.硒对黄曲霉毒素B1中毒雏鸭肾组织抗氧化功能的影响[J].中国兽医杂志, 2011, 47(12):19-21.

[19] 李亚琳.甲醛对小鼠肝脏毒性作用的研究[J].动物医学进展, 2013, 34(4):125-129.

[20] 王钟翊, 李前勇, 张德志.纳米硒对肉鸭日粮中黄曲霉毒素B1拮抗作用的研究[J].粮食与饲料工业, 2009(9):38-41.

[21] MOHIUDDIN S M.Effects of aflatoxin on immune response in viral diseases[J].Poultry Adviser, 1992(20/21/23/24):50-5363.

[22] 曹红, 尹逊慧, 陈善林, 等.黄曲霉毒素解毒酶对岭南黄肉仔鸡日粮中黄曲霉毒素B1解毒效果的研究[J].动物营养学报, 2010, 22(2):424-430.

[23] STANLEY V G, OJO R, WOLDESENBET S, et al.The use of Saccharomyces cerevisiae to suppress the effects of aflatoxcosis in broiler chicks[J].Poultry Science, 1993, 72(10):1867-1872.  

[24] ALPSOY L, YILDIRIM A, AGAR G.The antioxidant effects of vitamin A, C and E on aflatoxin B1-induced oxidative stress in human lymphocytes[J].Toxicology and Industrial Health, 2009, 25(2):121-127.  

[25] KOTAN E, ALPSOY L, ANAR M, et al.Protective role of methanol extract of Cetraria islandica (L.) against oxidative stress and genotoxic effects of AFB1 in human lymphocytes in vitro[J].Toxicology and Industrial Health, 2011, 27(7):599-605.

[26] WANG F Y, SHU G, PENG X, et al.Protective effects of sodium selenite against aflatoxin B1-induced oxidative stress and apoptosis in broiler spleen[J].Environmental Research and Public Health, 2013, 10(7):2834-2844.

[27] FORCEVILLE X.Effects of high doses of selenium, as sodium selenite, in septic shock patients a placebo-controlled, randomized, double-blind, multi-center phase Ⅱ study-selenium and sepsis[J].Journal of Trace Elements in Medicine and Biology, 2007, 21(Suppl.1):62-65.

[28] 曾昭惠, 张宗玉.自由基对线粒体DNA的氧化损伤与衰老[J].生物化学与生物物理进展, 1995, 22(5):429-432.

[29] KUMAR R, BALACHANDRAN C.Histopathological changes in broiler chickens fed aflatoxin and cyclopiazonic acid[J].Veterinarski Arhiv, 2009, 79(1):31-40.

[30] BRAHMI D, BOUAZIZ C, AYED Y, et al.Chemopreventive effect of cactus Opuntia ficus indica on oxidative stress and genotoxicity of aflatoxin B1[J].Nutrition and Metabolism, 2011, 8:73.  

[31] CHAN H T, CHAN C, HO J W.Inhibition of glycyrrhizic acid on aflatoxin B1-induced cytotoxicity in hepatoma cells[J].Toxicology, 2003, 188(2/3):211-217.

[32] SIES H.Oxidative stress:oxidants and antioxidants[J].Experimental Physiology, 1997, 82(2):291-295.

[33] KOBAYASHI M, YAMAMOTO M.Nrf2-Keap1 regulation of cellular defense mechanisms against electrophiles and reactive oxygen species[J].Advances in Enzyme Regulation, 2006, 46(1):113-140.  
文章导航

/