本试验旨在研制一种适合工业生产用的配方成本低、生长效果好的酵母菌液体培养基。试验以当地8种农副产品为主要原料，采用均匀试验方案设计培养基组方，通过测定液态培养基发酵后酵母活菌数及酵母多糖含量来选择最佳碳源和氮源，并通过确定复合碳源、碳氮比及无机盐添加量最终确定液态培养基的组方。结果表明：酵母菌液体培养基组方为无水葡萄糖0.9%、糖蜜4.5%、麸皮3.6%、玉米干酒糟及其可溶物0.3%、棉籽粕0.275%、酵母浸膏0.075%和（NH₃）₂SO₄ 0.08%，无机盐为KH₂PO₄ 0.3%、MgSO₄ 0.03%和CaCl₂ 0.03%，此时活菌数为2.38×10⁸ CFU/mL，β-葡聚糖和甘露聚糖含量分别为651.6和210.8 mg/L。由此可见，酵母培养液既适用于工业生产中酵母菌的快速生长繁殖，又能节约原料成本。

This experiment was conducted to develop a low-cost yeast liquid medium which was used in industrial production and had a good growth effect. In this study, eight kinds of local agricultural products were used as fermentation substrate. The components of the medium were designed by uniform design and the yeast viable count in the liquid medium after fermentation and the content of yeast polysaccharide was determined to select the best carbon source and nitrogen source. Then the composition of the liquid medium was determined by measuring the ratio of composite carbon source and composite nitrogen source and inorganic salts addition. The results showed that the composition of the yeast liquid culture included anhydrous glucose (0.9%), molasses (4.5%), wheat bran (3.6%), corn distillers dried grains with solubles (0.3%), cottonseed meal (0.275%), yeast extract (0.075%) and (NH₃)₂SO₄ (0.08%).The inorganic salts included KH₂PO₄ (0.3%), MgSO₄ (0.03%) and CaCl₂ (0.03%). The viable count was 2.38×10⁸ CFU/mL and the contents of β-glucan and mannan were 651.6 and 210.8 mg/L. In conclusion, the yeast liquid medium is not only good for the yeast rapid growth and reproduction in the industrial production but also saves raw materials costs.