刺五加多糖对免疫应激断奶仔猪免疫器官指数、粪便微生物菌群数量和胃肠道pH的影响

韩杰, 张飞, 边连全

doi:10.3969/j.issn.1006-267x.2014.08.036

动物营养学报 >

2014 , Vol. 26 >Issue 8: 2314 - 2319

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.08.036

研究简报 Short Communications

刺五加多糖对免疫应激断奶仔猪免疫器官指数、粪便微生物菌群数量和胃肠道pH的影响

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沈阳农业大学畜牧兽医学院, 沈阳 110866

韩杰（1978-），女，辽宁抚顺人，讲师，博士，从事动物营养生理调控研究。E-mail：hanjie1130@163.com

收稿日期: 2014-03-24

网络出版日期: 2014-08-13

基金资助

国家“十二五”科技支撑计划项目（2011BAD28B01-02）；2014辽宁省博士科研启动基金计划项目；科技部科技成果转化项目（2009GB2B000068）

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Effects of Acanthopanax senticosus Polysaccharide on Immune Organ Indexes, Counts of Fecal Microflora and Gastrointestinal pH of Immune Challenged Weaner Piglets

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College of Animal Husbandry and Veterinary, Shenyang Agricultural University, Shenyang 110866, China

Received date: 2014-03-24

Online published: 2014-08-13

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摘要

本试验旨在探讨饲粮添加刺五加多糖（ASPS）对大肠杆菌脂多糖（LPS）免疫应激断奶仔猪免疫器官指数、粪便微生物菌群数量及胃肠道pH的影响。试验采用2×2因素设计，将64头28日龄断奶、平均体重为（7.22±0.46）kg的“杜×长×大”仔猪随机分为4组，每组4个重复，每个重复4头猪。1、2组饲喂基础饲粮，3、4组饲喂在基础饲粮中添加800 mg/kg ASPS的饲粮，试验第14天，给1、3组仔猪注射生理盐水，2、4组仔猪注射等量的LPS，试验期21 d。结果表明：与饲喂基础饲粮的仔猪比较，饲喂添加ASPS仔猪的脾脏指数、胸腺指数和粪便乳酸杆菌数量分别显著提高了20.06%（P=0.012）、35.38%（P=0.019）和5.07%（P=0.043），使粪便大肠杆菌数量、回肠和盲肠内容物pH分别显著下降了6.04%（P=0.036）、3.62%（P=0.041）和2.74%（P=0.037）。ASPS与LPS的互作效应对脾脏指数（P=0.031）、胸腺指数（P=0.047）、粪便乳酸杆菌数量（P=0.050）、盲肠内容物pH（P=0.033）的影响显著。对于LPS免疫应激的仔猪，添加ASPS较饲喂基础饲粮的仔猪粪便大肠杆菌数量、回肠和盲肠内容物pH显著下降（P<0.05），粪便乳酸杆菌数量、胸腺和脾脏指数显著升高（P<0.05）。由此可见，饲粮添加ASPS具有促进免疫应激仔猪免疫器官发育和调节肠道健康的作用。

关键词： 刺五加多糖; 仔猪; 免疫器官指数; 免疫应激; 粪便微生物

本文引用格式

韩杰, 张飞, 边连全 . 刺五加多糖对免疫应激断奶仔猪免疫器官指数、粪便微生物菌群数量和胃肠道pH的影响[J]. 动物营养学报, 2014 , 26(8) : 2314 -2319 . DOI: 10.3969/j.issn.1006-267x.2014.08.036

Abstract

This experiment was conducted to evaluate the effect of dietary Acanthopanax senticosus polysaccharide (ASPS) on immune organ indexes, counts of fecal microflora and gastrointestinal pH of weaner piglets challenged with Escherichia coli lipopolysaccharide (LPS). A total of 64 Duroc×Large White×Landrace weaner piglets aged of 28 days with average initial body weight of (7.22±0.46) kg in a 2×2 factorial experiment were randomly allotted to 4 groups with 4 replications per group and 4 pigs per replication. Weaner piglets in 1 and 2 groups were fed basal diet, and those in 3, 4 groups were fed the basal diet added with 800 mg/kg ASPS. On 14 days, weaner piglets in 2 and 4 groups were injected with LPS and the other groups were given equivalent amount of saline. This experiment lasted for 21 days. The results showed as follows: compared with weaner piglets fed basal diet, dietary ASPS significantly increased the spleen, thymus indexes and the count of Lactobacillus in fecal by 20.06% (P=0.012), 35.38% (P=0.019) and 5.07% (P=0.043), respectively. Furthermore, ASPS supplementation significantly reducing effect on the count of Escherichia coli in fecal, the pH of ileum and cecum contents by 6.04% (P=0.036), 3.62% (P=0.041) and 2.74% (P=0.037), respectively. In addition, significant interactions were observed between ASPS and LPS in spleen (P=0.031), thymus indexes (P=0.047), the count of Lactobacillu in fecal (P=0.050) and the pH of cecum content (P=0.033). For the LPS challenge pigs, fed ASPS significantly reduced the count of Escherichia coli in fecal, the pH of ileum and cecum contents (P<0.05), while significantly increased the spleen and thymus indexes as well as the count of Lactobacillu in fecal compared with weaner piglets fed basal diet (P<0.05). It is concluded that dietary ASPS can improve the development of immune organ and modulate the health of intestinal tract of immune challenged weaner piglets.

Key words： Acanthopanax senticosus polysaccharide; weaner piglets; immune organ indexes; immune challenge; fecal microflora

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