研究简报 Short Communications

饲料中添加低聚木糖对凡纳滨对虾幼虾生长性能、非特异性免疫力、抗氧化功能及抗对虾白斑综合征病毒能力的影响

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  • 1. 广东省农业科学院动物科学研究所, 广州 510640;
    2. 广东省动物育种与营养公共实验室, 广州 510640;
    3. 广东省畜禽育种与营养研究重点实验室, 广州 510640
陈晓瑛(1987-),女,福建龙岩人,硕士研究生,从事水产动物营养与饲料科学研究。E-mail:xiaoyingchen08@126.com

收稿日期: 2014-03-03

  网络出版日期: 2014-08-13

基金资助

国家星火计划项目(2012GA780002);广东省自然科学基金团队对虾项目(10351064001000000);广州市科技计划项目(2011J4300089);广州市应用基础研究专项(2013J4100069)

Xylo-Oligosaccharides Supplementation Affects Growth Performance, Non-Specific Immunity, Antioxidant Function and Anti-White Spot Syndrome Virus Capacity of Juvenile Litopenaeus vannamei

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  • 1. Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
    2. Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, China;
    3. Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, China

Received date: 2014-03-03

  Online published: 2014-08-13

摘要

本试验旨在探讨低聚木糖(XOS)是否影响凡纳滨对虾的生长性能、非特异性免疫力、抗氧化功能及抗对虾白斑综合征病毒(WSSV)能力。试验选用平均体重为(0.67±0.02)g的凡纳滨对虾幼虾800尾,随机分成4组(每组4个重复,每个重复50尾),分别投喂添加0、200、400和600 mg/kg XOS的饲料,并分别记作G0(对照)、G200、G400和G600组。试验期为6周。6周饲养试验结束后,进行WSSV感染试验。结果表明:饲料中添加200 mg/kg XOS能显著提高对虾的增重率、特定生长率、蛋白质效率和蛋白质沉积率(P<0.05),并显著降低饲料系数(P<0.05)。饲料中添加XOS能提高对虾的血细胞总数(THC),且G400组显著高于对照组和G600组(P<0.05)。饲料中添加XOS能显著提高对虾血清酚氧化酶(PO)和溶菌酶(LZM)以及鳃LZM活力(P<0.05)。与对照组相比,G200组血清和肝胰腺超氧化物歧化酶(SOD)和过氧化物酶(POD)活力及血清总抗氧化能力(T-AOC)显著升高(P<0.05),各试验组肝胰腺T-AOC显著升高(P<0.05),G400组肝胰腺抗超氧阴离子自由基(Anti-O2-·)活力显著升高(P<0.05),各试验组肝胰腺丙二醛(MDA)含量以及G400和G600组血清MDA含量均显著降低(P<0.05)。对虾感染WSSV 72 h后,与对照组相比,各试验组对虾的累计死亡率均显著降低(P<0.05),G200、G400、G600组的相对保护率分别为15.2%、18.2%和15.2%。由此得出,饲料中添加适量的XOS能提高凡纳滨对虾幼虾的生长性能、非特异性免疫力、抗氧化功能和抗WSSV能力。

本文引用格式

陈晓瑛, 曹俊明, 黄燕华, 王国霞, 莫文艳, 陈冰, 赵红霞, 付晶晶 . 饲料中添加低聚木糖对凡纳滨对虾幼虾生长性能、非特异性免疫力、抗氧化功能及抗对虾白斑综合征病毒能力的影响[J]. 动物营养学报, 2014 , 26(8) : 2397 -2407 . DOI: 10.3969/j.issn.1006-267x.2014.08.047

Abstract

This experiment was aimed to study whether xylo-oligosaccharides (XOS) supplementation could affect non-specific immunity, antioxidant function and anti-white spot syndrome virus (WSSV) capacity of juvenile Litopenaeus vannamei. Eight hundred juvenile Litopenaeus vannamei with an initial body weight of (0.67±0.02) g were randomly assigned to 4 groups with 4 replicates per group and 50 shrimps per replicate. Shrimps in the 4 groups were fed diets supplemented with 0 (G0 group, as control group), 200 (G200 group), 400 (G400 group) and 600 mg/kg (G600 group) XOS for 6 weeks, respectively. After the 6 weeks' feeding trial, shrimps were injected with WSSV. The results showed that the weight gain rate (WGR), special gain rate (SGR), protein efficiency ratio (PER) and protein deposition rate (PDR) were significantly increased and the feed conversion ratio (FCR) was significantly decreased when adding 200 mg/kg in the diet (P<0.05). Dietary XOS could increase the total haemocyte count (THC), and that in G400 group was significantly higher than that in control group and G600 group (P<0.05). The activities of phenoloxidase (PO) and lysozyme (LZM) in serum and LZM in gill were significantly increased by adding XOS in the diet (P<0.05). Compared with control group, the activities of superoxide dismutase (SOD) and peroxidase (POD) in serum and hepatopancreas and serum total antioxidant capacity (T-AOC) in G200 group were significantly increased (P<0.05), the hepatopancreas T-AOC in experimental groups was significantly increased (P<0.05), and the activity of hepatopancreas anti-superoxide anion radical (Anti-O2-·) in G400 group was significantly increased (P<0.05), while the hepatopancreas malondialdehyde (MDA) content in G400 group and the serum MDA content in experimental groups were significantly reduced (P<0.05). After 72 h of WSSV injection, the cumulated mortality rate (CMR) in experimental groups was significantly decreased compared with control group (P<0.05), and the relative percentage survival (RPS) in G200, G400 and G600 groups was 15.2%, 18.2% and 15.2%, respectively. In conclusion, suitable amount of XOS in the diet can improve growth performance, non-specific immunity, antioxidant function and anti-WSSV capacity of juvenile Litopenaeus vannamei.

参考文献

[1] BALCÁZAR J L, DE BLAS I, RUIZ-ZARZUELA I, et al.The role of probiotics in aquaculture[J].Veterinary Microbiology, 2006, 114(3/4):173-186.

[2] GATESOUPE F J.The use of probiotics in aquaculture[J].Aquaculture, 1990, 180(1/2):147-165.

[3] THOMPSON F L, ABREU P C, CAVALLI R.The use of microorganisms as food source for Penaeus paulensis larvae[J].Aquaculture, 1999, 174(1/2):139-153.  

[4] 杨曙明.寡糖在动物营养研究中的进展[J].动物营养学报, 1999, 11(1):1-9.

[5] 郑建仙.功能性低聚糖[M].北京:化学工业出版社, 2004:67-85.

[6] TORRECILLAS S, MAKOL A, CABALLERO M J, et al.Immune stimulation and improved infection resistance in European sea bass (Dicentrarchus labrax) fed mannan oligosaccharides[J].Fish & Shellfish Immunology, 2007, 23(5):969-981.  

[7] 熊沈学, 刘文斌, 詹玉春, 等.低聚木糖梯度添加对异育银鲫生产性能的影响[J].饲料研究, 2007(7):60-62.

[8] 强俊, 王辉, 李瑞伟, 等.低聚木糖对奥尼罗非鱼幼鱼生长、体成分和消化酶活力的影响[J].淡水渔业, 2009, 39(6):63-68.

[9] 张荣斌, 曹俊明, 黄燕华, 等.饲料中添加低聚木糖对奥尼罗非鱼生长性能和血清生化指标的影响[J].动物营养学报, 2011, 23(11):2000-2008.

[10] 褚武英, 吴信, 成嘉, 等.低聚木糖对草鱼生长性能及血液生化指标的影响[J].饲料研究, 2008(6):60-61.

[11] 宋理平. 宝石鲈营养需求的研究[D].博士学位论文.山东:山东师范大学, 2009:159-170.

[12] 齐志涛, 张启焕, 仇明, 等.低聚木糖对斑点叉尾鮰生长及血液指标的影响[J].水产科学, 2011, 30(12):785-788.

[13] 胡毅.凡纳滨对虾饲料配方优化及几种饲料添加剂的应用[D].博士学位论文.青岛:中国海洋大学, 2007:133-150.

[14] 黄燕华, 王国霞, 刘襄河, 等.低聚木糖对凡纳滨对虾生长及消化道主要消化酶活性的影响[J].华南农业大学学报, 2010, 31(3):61-64.

[15] VARGAS-ALBORES F, GUZMÁN M A, OCHOA J L.A lipopolysaccharide-binding agglutinin isolated from brow shrimp (Penaeus californiensis Holmes) haemolymph[J].Comparative Biochemistry and Physiology, 1993, 104B(2):407-413.

[16] ASHIDA M.Purification and characterization of pre-phenoloxidase from hemolymph of the silkworm Bombyx mori[J].Archives of Biochemistry and Biophysics, 1971, 144(2):749-762.  

[17] 何建国, 周化民, 姚泊, 等.白斑综合症杆状病毒的感染途径和宿主种类[J].中山大学学报:自然科学版, 1999, 38(2):65-69.

[18] CORNICK J W, STEWART J E.Partial characterization of a natural agglutinin in the hemolymph of the lobster, Homarus americanus[J].Journal of Invertebrate Pathology, 1973, 21(3):255-262.  

[19] NICHOLS D S.Prokaryotes and the input of polyunsaturated fatty acids to the marine food web[J].Microbiology Letters, 2003, 219(1):1-7.  

[20] KIHARA M, SAKATA T.Production of short-chain fatty acids and gas from various oligosaccharides by gut microbes of carp (Cyprinus carpio L.) in micro-scale batch culture[J].Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology, 2002, 132(2):333-340.

[21] TANG Z R, YIN Y L, NYACHOTI C M, et al.Effect of dietary supplementation of chitosan and glacto-mannan-oligosaccharide on serum parameters and the insulin-like growth factor-Ⅰ mRNA expression in early-weaned piglets[J].Domestic Animal Endocrinology, 2005, 28(4):430-441.  

[22] RENGPIPAT S, RUKPRATANPORN S, PIYATIRATITIVORAKUL S, et al.Immunity enhancement in black tige shrimp (Penaeus monodon) by a probiont bacterium (Bacillus S11)[J].Aquaculture, 2000, 191(4):271-288.  

[23] RODRÍGUEZ J, LE MOLLAC G.State of the art of immunological tools and health control of penaeid shrimp[J].Aquaculture, 2000, 191(1/2/3):109-119.  

[24] LE MG, SOYEZ C, SAULNIER D, et al.Effect of hypoxic stress on the immune response and the resistance to vibriosis of the shrimp Penaeus stylirostris[J].Fish and Shellfish Immunology, 1998, 8(8):621-629.  

[25] 李振达, 陈小娥, 廖智, 等.壳寡糖对凡纳滨对虾生长和免疫力的影响[J].南方水产科学, 2011, 7(4):36-42.

[26] HERNÁNDEZ-LÓPEZ J, GOLLAS-GALVÁN T, VARGAS-ALBORES F.Activation of the prophenoloxidase system of the brown shrimp Penaeus californiensis Holmes[J].Comparative Biochemistry and Physiology Part C:Pharmacology, Toxicology and Endocrinology, 1996, 113(1):61-66.

[27] 刘含亮, 孙敏敏, 王红卫, 等.壳寡糖对虹鳟生长性能、血清生化指标及非特异性免疫功能的影响[J].动物营养学报, 2012, 2(3):479-486.

[28] 卢明淼, 陈孝煊, 吴志新, 等.果寡糖对草鱼非特异性免疫功能的影响[J].华中农业大学学报, 2010, 29(2):213-216.

[29] HESTA M, DEBRAEKELEER J, JANSSENS G P J, et al.The effect of a commercial high-fibre diet and an iso-malto-oligosaccharide-supplemented diet on post-prandial glucose concentrations in dogs[J].Journal of Animal Physiology and Animal Nutrition, 2001, 85(7/8):217-221.

[30] 李伟欣, 陈倩, 李平兰, 等.一种双歧杆菌胞外多糖免疫调节功能研究[J].微生物学通报, 2009, 36(6):931-935.

[31] FRANCIS R J, BORNET M D, FRED BROUNS.Immune-stimulating and gut health-promoting properties of short-chain fructo-oligosaccharides[J].Nutrition Reviews, 2002, 60(10):326-334.  

[32] CHESSON P.Multispecies competition in variable environments[J].Theoretical Population Biology, 1994, 45(3):227-276.  

[33] LEER R J, CHRISTIAENS H, VERSTRAETE W, et al.Gene disruption in Lactobacillus plantarum strain by site-specific recombination:isolation of a mutant strain deficient in conjugated bile salt hydrolase activity[J].Molecular and General Genetics, 1993, 239(1/2):269-272.

[34] 庞丽姣, 吴志新, 熊娟, 等.低聚木糖对草鱼非特异性免疫功能的影响[J].动物营养学报, 2010, 22(6):1687-1693.

[35] 王国霞, 周晔, 黄文庆, 等.低聚木糖对凡纳滨对虾生长、体组成和非特异免疫的影响[J].淡水渔业, 2010, 40(5):54-58.

[36] ORUC H H, CIBIK R, YILMAZ E, et al.Fate of aflatoxin M1 in Kashar cheese[J].Journal of Food Safety, 2007, 27(1):82-90.

[37] VARGAS-ALBORES F, GUZMÁN M, OCHOA J.An anticoagulant solution for haemolymph collection and prophenoloxidase studies of penaeid shrimp (Penaeus californiensis)[J].Comparative Biochemistry and Physiology, 1993, 106(2):299-303.  

[38] 王宝杰, 王雷.中国对虾血细胞吞噬活动中超氧阴离子(O2-)的产生[J].中国水产科学, 2003, 10(1):14-18.

[39] 扶国才, 罗有文, 周岩民.低聚木糖对生长猪生产性能和抗氧化功能的影响[J].江苏农业科学, 2009(1):221-222.

[40] 周伦江, 邵良平, 李国平, 等.甘露寡聚糖和粪链球菌对雏鸡和哺乳仔猪血液SOD和GSH-Px活性的影响[J].福建农业大学学报, 1999, 28(2):200-203.

[41] RAMADAN A, AFIFI N A, MOUSTA M M, et al.The effect of ascogen on the immune response of tilapia fish to Aeromonas hydrophila vaccine[J].Fish & Shellfish Immunology, 1994, 4(3):159-165.  

[42] MOHANKUMAR K, RAMASAMY P.White spot syndrome virus infection decreases the activity of antioxidant enzymes in Fenneropenaeus indicus[J].Virus Research, 2006, 115(1):69-75.  

[43] RAMESHTHANGAM P, RAMASAMY P.Antioxidant and membrane bound enzymes activity in WSSV infected Penaeus monodon Fabricius[J].Aquaculture, 2006, 254(1):32-39.

[44] MATHEW S, ASHOK KUMAR K, ANANDAN R, et al.Changes in tissue defence system in white spot syndrome virus (WSSV) infected Penaeus monodon[J].Comparative Biochemistry and Physiology Part C:Toxicology & Pharmacology, 2007, 145(3):315-320.  
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