动物营养学报 >

2014 , Vol. 26 >Issue 9: 2565 - 2573

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.09.016

禽营养 Poultry Nutrition

吡咯喹啉醌二钠对蛋鸡生产性能和鸡蛋胆固醇含量的影响

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  • 中国农业科学院饲料研究所, 农业部饲料生物技术重点开放实验室, 北京 100081
孙丽敏（1990-），女，内蒙古赤峰人，硕士研究生，动物营养与饲料科学专业。E-mail：sunlimin5024@163.com

收稿日期: 2014-04-18

  网络出版日期: 2014-09-10

基金资助

国家自然科学基金（31172212）；国家现代农业蛋鸡产业技术体系（CARS-41-K13）

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Effects of Pyrroloquinoline Quinone Disodium Salt on Performance and Egg Cholesterol Content of Laying Hens

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  • Key Laboratory of Feed Biotechnology of Ministry of Agricultural, Feed Research Institute, Chinese Academy of Agricultural Science, Beijing 100081, China

Received date: 2014-04-18

  Online published: 2014-09-10

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摘要

本试验旨在探讨吡咯喹啉醌二钠（PQQ·Na2）对蛋鸡生产性能、鸡蛋胆固醇含量和机体抗氧化能力的影响。选用360只23周龄的京红1号蛋鸡，随机分为5组，即0（对照组）、0.08、0.80、1.60 mg/kg PQQ·Na2组和100 mg/kg维生素E组，每组6个重复，每个重复12只鸡。试验期8周。结果表明：1）与对照组相比，PQQ·Na2能显著提高产蛋率（1.11%）（P<0.05），对料蛋比和蛋黄相对重量没有显著影响（P>0.05），显著增加鸡蛋的蛋白高度（P<0.05）。2）与对照组相比，0.80 mg/kg PQQ·Na2能显著提高肝脏胆固醇含量（P<0.05），显著降低血浆和全蛋胆固醇含量（P<0.05）；PQQ·Na2能显著提高血浆高密度脂蛋白胆固醇含量（P<0.05），对血浆低密度脂蛋白胆固醇含量无显著影响（P>0.05）。3）与对照组相比，0.80 mg/kg PQQ·Na2能显著降低血浆丙二醛含量（P<0.05），0.08 mg/kg PQQ·Na2能显著提高肝脏总超氧化物歧化酶活性和总抗氧化能力（P<0.05），对血浆总超氧化物歧化酶活性无显著影响（P>0.05）；与其他组相比，1.60 mg/kg PQQ·Na2显著提高了肝脏丙二醛含量（P<0.05）。综上，产蛋鸡饲粮中添加适宜水平（0.80 mg/kg）PQQ·Na2可以提高产蛋率，增强蛋鸡机体抗氧化能力，降低鸡蛋胆固醇含量。

关键词： 吡咯喹啉醌二钠; 产蛋鸡; 鸡蛋胆固醇; 抗氧化

本文引用格式

孙丽敏, 张海军, 武书庚, 岳洪源, 王晶, 齐广海 . 吡咯喹啉醌二钠对蛋鸡生产性能和鸡蛋胆固醇含量的影响[J]. 动物营养学报, 2014 , 26(9) : 2565 -2573 . DOI: 10.3969/j.issn.1006-267x.2014.09.016

Abstract

This experiment was conducted to evaluate the effects of dietary pyrroloquinoline quinone disodium salt (PQQ·Na2) on performance, egg cholesterol content and antioxidant capacity of laying hens. Three hundred and sixty Jinghong hens aged 23 weeks were randomly divided into 5 groups: basal diets with 0 (control group), 0.08, 0.80, 1.60 mg/kg PQQ·Na2 and 100 mg/kg vitamin E, respectivly. Each group contained 6 replicates with 12 hens per replicate. The experiment lasted for 8 weeks. The results showed as follows: 1) compared with control group, PQQ·Na2 significantly increased laying rate (1.11%) (P<0.05), had no significant effects on egg to feed ratio and egg relative weight (P>0.05), but significantly increased albumen height(P<0.05). 2) Compared with control group, 0.80 mg/kg PQQ·Na2 significantly increased liver cholesterol content (P<0.05), and significantly decreased cholesterol content in plasma and egg (11.27%) (P<0.05). PQQ·Na2 significantly increased plasma high density lipoprotein cholesterol content (P<0.05), but had no significant effect on plasma low density lipoprotein cholesterol content (P>0.05). 3) Compared with control group, 0.80 mg/kg PQQ·Na2 decreased plasma methane dicarboxylic aldehyde (MDA) content (P<0.05) and 0.08 mg/kg PQQ·Na2 significantly increased liver total superoxide dismutase (T-SOD) activity and total antioxidant capacity(P<0.05), but had no significant effect on plasma T-SOD activity (P>0.05). Compared with the other groups, 1.60 mg/kg PQQ·Na2 significantly increased MDA content in liver (P<0.05). In conclusion, this study indicates that the appropriate level of dietary PQQ·Na2 (0.80 mg/kg) increases laying rate, enhances antioxidant capacity of laying hens and decreases egg cholesterol content.

Key words： PQQ·; Na2; laying hens; egg cholesterol; antioxidant

参考文献

[1] ZHANG P,XU Y P,SUN J X,et al.Protection of pyrroloquinoline quinone against methylmercury-induced neurotoxicity via reducing oxidative stress[J].Free Radical Rearch,2009,43(3):224-233.  

[2] TSUCHIDA T H,YASUYAMA T F,HIGUCHI K H,et al.The protective effect of pyrroloquinoline quinone and its derivatives against carbon tetrachloride-induced liver injury of rats[J].Journal of Gastroenterology and Hepatology,1993,8(4):342-347.  

[3] BAUERLY K,HARRIS C,CHOWANADISAI W,et al.Altering pyrroloquinoline quinone nutritional status modulates mitochondrial,lipid,and energy metabolism in rats[J].PLoS One,2011,6(7):e21779.

[4] 徐磊,张海军,武书庚,等.吡咯喹啉醌对蛋鸡生产性能、蛋品质及抗氧化功能的影响[J].动物营养学报,2011,23(8):1370-1377.

[5] SAHIN N,SAHIN K,ONDERCI M,et al.Effects of dietary lycopene and vitamin E on egg production,antioxidant status and cholesterol levels in Japanese quail[J].Asian-Australasian Journal of Animal Sciences,2006,19(2):224-230.

[6] FOLCH J,LEES M,SLOANE STANLEY G H.A simple method for the the isolation and purification of total lipides from animal tissues[J].The Journal of Biological Chemistry,1957,226(1):497-509.

[7] 尹靖东.类黄酮对鸡蛋胆固醇及其氧化物形成的影响[D].博士学位论文.北京:中国农业科学院,2000.

[8] 江均平,蔡丽君,卢卫东,等.利用酶法简捷测定鸡蛋总胆固醇含量研究[J].畜牧与兽医,2010,42(5):37-39.

[9] STEINBERG F,STITES T E,ANDERSON P,et al.Pyrroloquinoline quinone improves growth and reproductive performance in mice fed chemically defined diets [J].Experimental Biology and Medicine,2003,228(2):160-166.

[10] RADWAN NADIA L,HASSAN R A,QOTA E M,et al.Effect of natural antioxidant on oxidative stability of eggs and productive and reproductive performance of laying hens[J].International Journal of Poultry Science,2008,7(2):134-150.  

[11] ÇIFTÇI M,ERTAS O N,GVLER T.Effects of vitamin E and vitamin C dietary supplementation on egg production and egg quality of laying hens exposed to a chronic heat stress[J].Revue de Medecine Veterinaire,2005,156(2):107-111.

[12] MOHITI-ASLI M,SHARIATMADARI F,LOTFOLLAHIAN H,et al.Effects of supplementing layer hen diets with selenium and vitamin E on egg quality,lipid oxidation and fatty acid composition during storage[J].Canadian Journal of Animal Science,2008,88(3):475-483.  

[13] QI G H,SIM J S.Natural tocopherol enrichment and its effect in n-3 fatty acid modified chicken eggs[J].Journal of Agricultural and Food Chemistry,1998,46(5):1920-1926.  

[14] 元娜.万寿菊提取物和维生素E对鸡蛋品质、抗氧化能力及免疫应答影响的研究[D].硕士学位论文.保定:河北农业大学,2009.

[15] TOTH P P,DAVIDSON M H.Therapeutic interventions targeted at the augmentation of reverse cholesterol transport[J].Current Opinion in Cardiology,2004,19(4):373-379.

[16] CHOWANADISAI W,BAUERLY K A,TCHAPARIAN E,et al.Pyrroloquinoline quinone stimulates mitochondrial biogenesis through cAMP response element-binding protein phosphorylation and increased PGC-1α expression[J].The Journal of Biological Chemistry,2010,285(1):142-152.  

[17] SHIN D J,CAMPOS J A,GIL G,et al.PGC-1αactivates CYP7A1 and bile acid biosynthesis[J].The Journal of Biological Chemistry,2003,278(50):50047-50052.  

[18] ZHU B Q,ZHOU H Z,TEERLINK J R,et al.Pyrroloquinoline quinone (PQQ) decreases myocardial infarct size and improves cardiac function in rat models of ischemia and ischemia/reperfusion[J].Cardiovascular Drug and Therapy,2004,18(6):421-431.  

[19] OHWADA K,TAKEDA H,YAMAZAKI M,et al.Pyrroloquinoline quinone (PQQ) prevents cognitive deficit caused by oxidative stress in rats[J].Journal of Clinical Biochemistry and Nutrition,2008,42(1):29-34.  

[20] SAHIN N,SAHIN K,ONDERCI M.Vitamin E and selenium supplementation to alleviate cold-stress-associated deterioration in egg quality and egg yolk mineral concentrations of Japanese quails[J].Biological Trace Element Research,2003,96(1/2/3):179-189.

[21] ISHII T,AKAGAWA M,NAITO Y,et al.Pro-oxidant action of pyrroloquinoline quinone:characterization of protein oxidative modifications[J].Bioscience,Biotechnology and Biochemistry,2010,74(3):663-666.  

[22] SUGIOKA K,NAKANO M,NAITO I,et al.Properties of a coenzyme,pyrroloquinoline quinone:generation of an active oxygen species during a reduction-oxidation cycle in the presence of NAD(P)H and O2[J].Biochimica et Biophysica Acta (BBA):General Subjects,1988,964(2):175-182.  

[23] WATANABE A,HOBARA N,OHSAWA T,et al.Nephrotoxicity of pyrroloquinoline quinone in rats[J].Hiroshima Journal of Medical Sciences,1989,38(1):49-51.

[24] NAKANO M,SUZUKI H,IMAMURA T,et al.Genotoxicity of pyrroloquinoline quinone (PQQ) disodium salt (BioPQQTM)[J].Regulatory Toxicology and Pharmacology,2013,67(2):189-197.  
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