沙棘嫩枝叶对绵羊瘤胃发酵特性和血清免疫指标的影响

刘艳丰, 唐淑珍, 张文举, 侯广田, 黄仁慧, 聂存喜, 王文奇

doi:10.3969/j.issn.1006-267x.2014.09.020

动物营养学报 >

2014 , Vol. 26 >Issue 9: 2599 - 2606

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.09.020

反刍与草食动物营养 Ruminants and Herbivorous Animal Nutrition

沙棘嫩枝叶对绵羊瘤胃发酵特性和血清免疫指标的影响

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1. 新疆畜牧科学院饲料研究所, 乌鲁木齐 830000;
2. 石河子大学动物科技学院, 石河子 832000;
3. 新疆生产建设兵团第二师铁门关市畜牧兽医站, 铁门关 841005

刘艳丰（1981-），男，湖南临湘人，助理研究员，博士，研究方向为动物营养与饲料营养。E-mail：liuyanfeng520@sina.com

收稿日期: 2014-04-18

网络出版日期: 2014-09-10

基金资助

新疆维吾尔自治区公益性科研院所基本科研业务经费资助项目（KY2013008）；国家现代肉羊产业技术体系（nycytx-39）

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Shoots and Leaves of Sea Buckthorn: Effects on Rumen Fermentation Characteristics and Serum Immune Indices of Sheep

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1. Feed Research Institute, Xinjiang Academy of Animal Science, Urumqi 830000, China;
2. College of Animal Science and Technology, Shihezi University, Shihezi 832000, China;
3. Tiemenguan Animal Husbandry and Veterinary Station, The Xinjiang Production and Construction Corpssecond Division, Tiemenguan 841005, China

Received date: 2014-04-18

Online published: 2014-09-10

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摘要

本试验旨在研究沙棘嫩枝叶对绵羊瘤胃发酵特性和血清免疫指标的影响。采用单因素随机区组试验设计，选择体重为（28.0±3.5） kg的阿勒泰羔羊60只，随机分成4组，每组3个重复，每个重复5只。对照（Con）组饲喂基础饲粮，试验组以2.5%（SJZY-1组）、5.0%（SJZY-2组）和7.5%沙棘嫩枝叶（SJZY-3组）等量替换基础饲粮中麦秸。试验期63 d，其中预试期7 d，正试期56 d。在正试期第56天晨饲前，分别从各组每个重复随机选取2只，采集血样和瘤胃液。结果表明：1）SJZY-1组和SJZY-3组平均日增重显著高于Con组（P<0.05）。2）随沙棘嫩枝叶添加量的增加，瘤胃液pH呈下降趋势，组间无显著差异（P>0.05）；试验组乙酸、丙酸、丁酸和总挥发性脂肪酸（TVFA）的浓度均极显著高于Con组（P<0.01），SJZY-3组最高；乙酸/丙酸和乙酸/TVFA没有显著变化（P>0.05）；SJZY-2组和SJZY-3组氨态氮浓度分别显著（P<0.05）和极显著（P<0.01）低于Con组。3）与Con组相比，沙棘嫩枝叶的添加对血清CD4、白细胞介素1（IL-1）、白细胞介素2（IL-2）浓度的影响为低量抑制中、高量促进，而对血清γ干扰素（γ-IFN）浓度的影响则表现为低量促进。结果提示，沙棘嫩枝叶能提高绵羊平均日增重，增加瘤胃液中挥发性脂肪酸浓度和降低氨态氮浓度，提高血清CD4、IL-1、IL-2、γ-IFN浓度；综合考虑，建议沙棘嫩枝叶在绵羊饲粮中以5.0%~7.5%添加。

关键词： 沙棘嫩枝叶; 阿勒泰羊; 挥发性脂肪酸; 氨态氮; 细胞免疫因子

本文引用格式

刘艳丰, 唐淑珍, 张文举, 侯广田, 黄仁慧, 聂存喜, 王文奇 . 沙棘嫩枝叶对绵羊瘤胃发酵特性和血清免疫指标的影响[J]. 动物营养学报, 2014 , 26(9) : 2599 -2606 . DOI: 10.3969/j.issn.1006-267x.2014.09.020

Abstract

This experiment was conducted to study the effects of shoots and leaves of sea buckthorn on rumen fermentation characteristics and serum immune indices of sheep. A single factor random block design was used, and sixty healthy Altay lambs with the body weight of (28.0±3.5) kg were selected and randomly divided into 4 groups with 3 replicates in each group and 5 lambs per replicate. Lams in control (Con) group were fed a basal diet, and wheat straw in the basal diet was replaced by shoots and leaves of sea buckthorn at 2.5% (SJZY-1 group), 5.0% (SJZY-2 group) and 7.5% (SJZY-3 group), respectively, in test groups. Trial lasted for 63 days, which consisted of a 7-day-pretrial and a 56-day-formal trial. On day 56 of formal trial, two lambs per replicate in each group were randomly selected to collect blood and rumen fluid samples before morning feeding. The results showed as follows: 1) average daily gain in SJZY-1 and SJZY-3 groups was significantly higher than that in Con group (P<0.05). 2) Rumen fluid pH tended to be decreased with the increase of supplemental level of shoots and leaves of sea buckthorn, but no significant difference was found among groups (P>0.05); the concentrations of acetic acid, propionic acid, butyric acid and total volatile fatty acids (TVFA) in test groups were significantly higher than those in Con group (P<0.01), and SJZY-3 group had the highest values; acetic acid/propionic acid and acetic acid/TVFA were not significantly changed (P>0.05); compared with Con group, ammonia nitrogen (NH₃-N) concentration in SJZY-2 (P<0.05) and SJZY-3 groups (P<0.01) was significantly decreased. 3) Compared with Con group, the effects of the supplementation of shoots and leaves of sea buckthorn on serum CD4, interleukin-1 (IL-1) and interleukin-2 (IL-2) concentrations were low-dose inhibition and medium and high-dose stimulation, while the effect on serum γ-interferon (γ-IFN) was low-dose stimulation. The results indicate that shoots and leaves of sea buckthorn can improve average daily gain, increase volatile fatty acid concentrations and reduce NH₃-N concentration in rumen fluid, and increase serum CD4, IL-1, IL-2 and γ-IFN concentrations; the recommended supplemental level of shoots and leaves of sea buckthorn is 5.0% to 7.5% in diet for sheep.

Key words： shoots and leaves of sea buckthorn; Altay sheep; volatile fatty acid; ammonia nitrogen; cellular immune factor

参考文献

[1] ZHAO Y Z,WU F H.Seabuckthorn flavonoids and their medical value[J].Hippophae,1997,10(1):39-41.

[2] 李繁.沙棘的研究进展[J].中国医药导报,2009,6(1):7-9.

[3] 邵淑丽,徐兴军,邵会祥,等.沙棘嫩枝叶对鸡生长性能及肉质的影响[J].黑龙江畜牧兽医,2002(5):12-13.

[4] 郭福存,王建国,谢家声,等.沙棘叶和沙棘果肉渣对奶山羊泌乳性能的影响[J].中兽医医药杂志,1990(3):9-11.

[5] FANG X K,GAO J,ZHU D N.Kaempferol and quercetin isolated from Euonymus alatus improve glucose uptake of 3T3-LI cells without adipogenesis activity[J].Life Science,2008,82 (11/12):615-622.

[6] 刘艳丰,孙焕林,唐淑珍,等.沙棘的理化性质及其在家禽生产中的应用[J].中国畜牧兽医,2013,40(3):247-251.

[7] 程文杰,潘晓亮,潘永斌,等.不同处理方法的人工栽培沙棘枝叶对绵羊育肥效果的影响[J].饲料研究,2013(4):40-43.

[8] PRAKASH H,BALA M,A1 I A,et al.Modification of gamma radiation induced response of peritoneal macrophages and splenocytes by Hippophae rhamnoides(RH-3)in mice[J].Journal of Pharmacy and Pharmacology,2005,57(8):1065-1072.

[9] 徐兴军,邵淑丽,张伟伟,等.沙棘饲料添加剂饲喂肉鸡的效果[J].江苏农业科学,2012,40(12):210-212.

[10] MARTEN V H.Challenging the retinal for altering VFA ration in growing ruminates[J].Feed Mix,l996,4(1):68-70.

[11] 中华人民共和国农业部.NY/T 816-2004肉羊饲养标准[S].北京:中国标准出版社,2004.

[12] 秦为琳.用气相色谱测定瘤胃挥发性脂肪酸方法的研究改进[J].南京农业大学报,1982(5):110-116.

[13] 郭旭东.芦丁对奶牛泌乳性能、瘤胃消化代谢和对大鼠乳腺发育的影响[D].硕士学位论文.北京:中国农业科学院,2011.

[14] 阮成江.沙棘叶的饲料价值及开发利用[J].陕西林业科技,2002(3):26-30.

[15] 邵淑丽,徐兴军,赵军,等.沙棘嫩枝叶对鸡日粮中蛋白质利用率的影响[J].高师理科学刊,2001,21(2):47-49.

[16] 习文东,雷天民,张付舜.沙棘在畜牧兽医上的应用研究[J].沙棘,1988,1(4):45.

[17] 李旺.瘤胃挥发性脂肪酸的作用及影响因素[J].中国畜牧杂志,2012,48(7):63-66.

[18] 刘洁,刁其玉,赵一广,等.饲粮不同NFC/NDF对肉用绵羊瘤胃pH、氨态氮和挥发性脂肪酸的影响[J].动物营养学报,2012,24(6):1069-1077.

[19] 邵卫星,彭大新,卢建红,等.鸡的7种细胞因子的一些分子生物学特性[J].动物医学进展,2004,25(4):11-15.

[20] WILLIAMS N H,STAHLY T S,ZIMMERMAN D R.Effect of chronic immune system activation on body nitrogen retention,partial efficiency of lysine utilization,and lysine needs of pigs[J].Journal of Animal Science,1997,75(9):2472-2480.

[21] 李垚,金赛勉,刘长伟.沙棘提取物对断奶仔猪的免疫调节作用[J].沙棘,2009,22(1):4-6.

[22] TANG W F,WANG Y G,ZHU L,et al.Effect of somatostatin on immune inflammatory response in patients with severe acute pancreatitis[J].Journal of Digestive Diseases,2007,8(2):96-102.

[23] HILTON L S,BEAN A G D,KIMPTON W G,et al.Interleukin-2 directly induces activation and pro liferation of chicken T cells in vivo[J].Journal Interferon and Cytokine Research,2002,22(7):755-763.

[24] SCHIJNS V E,WEINING K C,NUIJTEN P,et al.Immunoad-juvant activities of E.coli and plasmid expressed recombinant chicken IFN-α/β in 1-day and 3-week old chickens[J].Vaccine,2000,18:2147-2154.

[25] LWENTHAL J W,O NEIL T E,BROADWAY M,et al.Coadministration of IFN-γenhances antibody responses in chickens[J].Journal Interferon and Cytokine Research,1998,18(8):617-622.

[26] GEETHA S,SINGH V,RAM M S,et al.Immunomodulatory effects of seabuckthom(Hippophoe rhamnoides L.)agaimt chromium(Ⅵ)induced im-munosuppression[J].Molecular and Cellular Biochemistry,2005,278(1/2):101-109.

[27] 邵卫星,卢建红,彭大新,等.鸡IL-1β、IL-2、IFN-γ和MGF对表达新城疫病毒HN基因的重组鸡痘病毒免疫效力的影响[J].中国兽医学报,2008,28(5):506-510.

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