本试验采用单因素试验设计，通过在基础饲料中添加0（对照）、0.025%、0.050%、0.100%、0.200%和0.400%的纤维素酶，配制6种试验饲料，饲喂平均初始体重为（1.44±0.01） g的幼刺参49 d，以研究饲料中添加纤维素酶对幼刺参生长性能、消化能力和非特异性免疫力的影响。每组设3个重复，每个重复35头刺参。结果表明：饲料中添加0.025%~0.100%纤维素酶可显著提高幼刺参的特定生长率（SGR）（P<0.05），降低饲料系数（FCR）（P<0.05），其中0.100%组的SGR最高和FCR最低。饲料中添加纤维素酶对幼刺参的摄食率（FI）和成活率（SR）无显著影响（P>0.05）。饲料干物质表观消化率（ADCd）和粗蛋白质表观消化率（ADCp）随着纤维素酶添加量的增加先上升后下降，其中0.100%组的ADCd和ADCp最高，显著高于对照组（P<0.05），0.400%组的ADCd与对照组差异不显著（P>0.05），但ADCp显著低于对照组（P<0.05）。饲料中添加纤维素酶后幼刺参前肠、中肠和后肠淀粉酶（AMS）活性均有所升高，其中0.100%、0.200%和0.400%的纤维素酶分别对前肠、中肠和后肠AMS活性作用显著（P<0.05）。饲料中添加0.100%~0.200%纤维素酶可显著提高幼刺参肠道中超氧化物歧化酶（SOD）和酸性磷酸酶（ACP）活性（P<0.05）。由此得出，在本试验条件下，饲料中添加纤维素酶可促进幼刺参生长，提高其消化能力和非特异性免疫力。综合考虑生长、消化和非特异性免疫指标，在幼刺参饲料中纤维素酶的适宜添加量为0.100%。

The experiment was conducted to investigate the effects of dietary cellulase on growth performance, digestion ability and non-specific immunity of juvenile sea cucumber (Apostichopus japonicus Selenka). Juvenile sea cucumbers with an average initial body weight of (1.44±0.01) g were fed with 6 experimental diets according to a single factor design: a basal diet supplemented with 0 (control), 0.025%, 0.050%, 0.100%, 0.200% and 0.400% cellulase for 49 days. There were 3 replicates per group with 35 sea cucumbers. The results showed as follows: supplementation of 0.025% to 0.100% cellulase could significantly enhance the specific growth rate (SGR) (P<0.05) and significantly reduce the feed conversion ratio (FCR) (P<0.05), and the highest SGR and lowest FCR of juvenile sea cucumbers were both found in 0.100% group. There were no significant differences in feed intake (FI) and survival rate (SR) among all groups (P>0.05). With the cellulase supplemental level increasing, the apparent digestibility coefficient of dry matter (ADCd) and the apparent digestibility coefficient of crude protein (ADCp) in diets were firstly raised and then declined, and the highest ADCd and ADCp were both found in 0.100% group which were significantly higher than those in control group (P<0.05). The ADCd in 0.400% group was not significantly different compared with control group (P>0.05), but the ADCp in 0.400% group was significantly lower than that in control group (P<0.05). The activity of amylase (AMS) in foregut, midgut and hindgut of juvenile sea cucumbers was increased by cellulase supplementation, and the supplementation of 0.100%, 0.200% and 0.400% cellulase had significant influences on AMS activity in foregut, midgut and hindgut, respectively (P<0.05). Supplementation of 0.100% to 0.200% cellulase could significantly increase the activities of superoxide dismutase (SOD) and acid phosphatase (ACP) in gut of juvenile sea cucumbers (P<0.05). It can be concluded that dietary cellulase can promote the growth, and enhance the digestion ability and non-specific immunity of juvenile sea cucumber in the condition of this experiment. It compromises the indexes on growth, digestion and non-specific immune, the optimal supplemental level of cellulase in the diet of juvenile sea cucumber is 0.100%.