动物营养学报 >

2014 , Vol. 26 >Issue 9: 2714 - 2721

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.09.033

特种经济动物营养 Special Economic Animal Nutrition

低蛋白质饲粮添加蛋氨酸和赖氨酸对离乳期梅花鹿生长性能和血清生化指标的影响

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  • 中国农业科学院特产研究所, 特种经济动物分子生物学国家重点实验室, 长春 130112
黄健（1989-），男，四川德阳人，硕士研究生，从事特种经济动物营养与饲养研究。E-mali：hj6503310@vip.qq.com

收稿日期: 2014-03-19

  网络出版日期: 2014-09-10

基金资助

国家科技支撑计划专项“名优动物药梅花鹿产业发展关键技术研究”（2011BAI03B02）

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Effects of Low Protein Diet Supplemented Lysine and Methionine on Growth Performance and Serum Biochemical Indices of Weaner Sika Deer

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  • State Key Laboratory of Special Economic Animal Molecular Biology, Institute of Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, Changchun 130112, China

Received date: 2014-03-19

  Online published: 2014-09-10

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摘要

本试验旨在研究低蛋白质饲粮中添加蛋氨酸（Met）和赖氨酸（Lys）对离乳期梅花鹿仔鹿生长性能和血清生化指标的影响。选择16只健康3月龄雄性梅花鹿，随机分为4个组，每组4个重复，每个重复1只。各组限量饲喂4种不同饲粮：对照组（Ⅰ组）饲喂16.28%粗蛋白质（CP）的高蛋白质饲粮，试验组饲喂13.40%CP添加0.23%Lys并分别添加0（Ⅱ组）、0.06%（Ⅲ组）、0.12%（Ⅳ组）Met的低蛋白质饲粮，试验期为56 d。结果表明：1）Ⅱ组平均日增重（ADG）最低，Ⅱ、Ⅲ、Ⅳ组ADG随饲粮Met水平增加而增加，Ⅳ组ADG与Ⅰ组基本相同。2）Ⅰ组56 d血清白蛋白（ALB）含量显著高于Ⅱ、Ⅲ组（P<0.05），56 d血清总蛋白（TP）含量显著高于Ⅱ组（P<0.05），28 d血清尿素氮（UN）含量极显著高于Ⅳ组（P<0.01），56 d血清葡萄糖（GLU）含量极显著低于Ⅱ、Ⅲ组（P<0.01），血清天冬门氨酸氨基转移酶（AST）活性显著高于Ⅳ组（P<0.05），28 d血清碱性磷酸酶（AKP）活性显著或极显著高于Ⅱ、Ⅲ组（P<0.05或P<0.01）。3）Ⅱ、Ⅲ、Ⅳ组相比，Ⅳ组28 d血清UN含量显著低于Ⅱ组（P<0.05），Ⅳ组28 d血清AKP活性显著高于Ⅱ、Ⅲ组（P<0.05），但血清球蛋白（GLB）、TP含量随饲粮Met水平升高而增加，血清AST、丙氨酸氨基转移酶（ALT）活性和UN含量随饲粮Met水平升高而降低。由此可见，在13.40%CP的饲粮中添加Lys和Met，仔鹿的ADG可达到16.28%CP饲粮的水平，饲粮中添加0.12%Met有利于仔鹿生长和代谢平衡。

关键词： 梅花鹿; 低蛋白质; 赖氨酸; 蛋氨酸; 生长性能; 血清生化指标

本文引用格式

黄健, 鲍坤, 张铁涛, 李光玉, 周宁, 王凯英, 杨福合 . 低蛋白质饲粮添加蛋氨酸和赖氨酸对离乳期梅花鹿生长性能和血清生化指标的影响[J]. 动物营养学报, 2014 , 26(9) : 2714 -2721 . DOI: 10.3969/j.issn.1006-267x.2014.09.033

Abstract

This experiment was conducted to investigate the effects of low protein diet supplemented lysine (Lys) and methionine (Met) on growth performance and serum biochemical indices of weaner sika deer. Sixteen healthy 3-month-old male sika deer were randomly divided into 4 groups with 4 replicates per group and 1 sika deer per replicate. Restricted feeding 4 diets: control group (group Ⅰ) fed 16.28% crude protein (CP) based diet, and test groups fed 13.40 % CP diet supplemented with 0.23% Lys and 0 (group Ⅱ), 0.06% (group Ⅲ), 0.12% (group Ⅳ) Met, respectively. The results showed as follows: 1) the average daily gain (ADG) in group Ⅱ was the lowest, and the ADG in groups Ⅱ, Ⅲ and Ⅳ was increased with dietary Met level increased, the ADG in group Ⅳ was nearly the same as that in group Ⅰ(P>0.05). 2) The serum albumin (ALB) content on 56 days in group Ⅰ was significantly higher than that in groups Ⅱ and Ⅲ (P<0.05), the serum total protein (TP) content on 56 days was significantly higher than that in group Ⅱ (P<0.05), the serum urea nitrogen (UN) content on 28 days was significantly higher than that in group Ⅳ (P<0.01), the serum glucose (GLU) content on 56 days was significantly lower than that in groups Ⅱ and Ⅲ (P<0.01), the serum aspartic transaminase (AST) activity was significantly higher than that in group Ⅳ (P<0.05), the serum alkaline phosphatase (AKP) activity on 28 days was significantly higher than that in groups Ⅱ and Ⅲ (P<0.05 or P<0.01). 2) Compared among groups Ⅱ, Ⅲ and Ⅳ, the serum UN content on 28 days in group Ⅱ was significantly higher than that in group Ⅳ (P<0.05), the AKP activity in group Ⅳ on 28 days was significantly higher than that in groups Ⅱ and Ⅲ (P<0.05), but serum ALB and TP contents increased with Met level increased, serum AST and ALT activities and UN content decreased with Met level increased. In conclusion, the ADG of sika deer fed diet supplemented Lys and Met with a CP level of 13.40% is the same as that fed diet with a CP level of 16.28%. The supplemented 0.12% Met in diets can enhance growth and metabolism balance of sika deer.

Key words： sika deer; low protein; lysine; methionine; growth performance; serum biochemical indices

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