分子营养 Molecular Nutrition

聚乙二醇修饰猪胰高血糖素样肽-2对试验性结肠炎小鼠肠道紧密连接蛋白和炎性因子基因表达的影响

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  • 浙江省农业科学院畜牧兽医研究所, 杭州 310021
齐珂珂(1981-),女,河南偃师人,助理研究员,博士,主要从事畜禽健康养殖的研究。E-mail:nkyqkk@163.com

收稿日期: 2014-04-28

  网络出版日期: 2014-09-10

基金资助

现代农业产业技术体系(CARS-36);国家青年科学基金项目(C170105/31101724)

Effects of Polyethylene Glycosylation Porcine Glucagon-Like Peptide-2 on Gene Expression of Tight Junction Proteins and Inflammatory Cytokines in a Murine Model of Experimental Colitis

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  • Institute of Animal Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received date: 2014-04-28

  Online published: 2014-09-10

摘要

本试验旨在研究聚乙二醇(PEG)修饰猪胰高血糖素样肽-2(pGLP-2)对结肠炎小鼠肠道紧密连接蛋白和炎性因子基因表达的影响。试验选取24只BALB/C小鼠,随机分为4组,葡聚糖硫酸钠(DSS)组小鼠饮用3% DSS建立小鼠结肠炎模型,DSS+pGLP-2组和DSS+PEG-pGLP-2组饮用3% DSS且在试验第8天腹腔分别注射pGLP-2和PEG-pGLP-2,饮水组小鼠正常饮水。试验期10 d。结果表明:与饮水组相比,DSS组小鼠结肠紧密连接闭锁小带基因(ZO-1)的mRNA相对表达量极显著降低(P<0.01);与DSS组相比,注射pGLP-2对ZO-1的mRNA相对表达量没有改善(P>0.05),而注射PEG-pGLP-2可极显著增加ZO-1的mRNA相对表达量(P<0.01)。与饮水组相比,DSS组小鼠结肠白细胞介素-6(IL-6)、白细胞介素-10(IL-10)和干扰素-γ基因(INF-γ)的mRNA相对表达量极显著增加(P<0.01);与DSS组相比,注射pGLP-2和PEG-pGLP-2可极显著降低IL-6、IL-10和IFN-γ的mRNA相对表达量(P<0.01)。结果提示,PEG-pGLP-2通过增加肠道紧密连接蛋白的表达、降低结肠炎小鼠炎性细胞因子的表达抑制其炎性病变,且作用效果优于pGLP-2。

本文引用格式

齐珂珂, 吴杰, 徐子伟 . 聚乙二醇修饰猪胰高血糖素样肽-2对试验性结肠炎小鼠肠道紧密连接蛋白和炎性因子基因表达的影响[J]. 动物营养学报, 2014 , 26(9) : 2745 -2751 . DOI: 10.3969/j.issn.1006-267x.2014.09.037

Abstract

This study aimed to evaluate the effects of polyethylene glycosylation (PEGylation) porcine glucagon-like peptide-2 (pGLP-2) (PEGylation pGLP-2) on gene expression of tight junction proteins and inflammatory cytokines in a murine model of experimental colitis. Twenty-four BALB/C mice were randomly assigned into four groups: dextran sulfate sodium (DSS, mice colitis model by drinking 3% DSS), DSS+pGLP-2 (3% DSS by drinking+pGLP-2 by intraperitoneal injection on the 8th day), DSS+PEG-pGLP-2 (3% DSS by drinking+PEG-pGLP-2 by intraperitoneal injection on the 8th day) and water groups for 10 days. The results showed as follows: compared with the water group, DSS significantly decreased the mRNA expression level of zonula occludens-1 (ZO-1) in colon (P<0.01). Specifically, PEG-pGLP-2, nor pGLP-2 injection (P>0.05) significantly increased the ZO-1 mRNA expression level compared with the DSS group (P<0.01). Compared with the water group, DSS group significantly increased the mRNA expression levels of interleukin-6 (IL-6), interleukin-10 (IL-10) and interferon-γ (INF-γ) in colon (P<0.01). And PEG-pGLP-2 and pGLP-2 injection significantly decreased the IL-6, IL-10 and INF-γ mRNA expression levels compared with the DSS group (P<0.01). These results show that PEG-pGLP-2 reduce the severity of colonic injury in murine colitis by increasing the expression of the tight junction proteins and reducing the expression of inflammatory cytokines. The therapy of PEG-pGLP-2 is better than that of pGLP-2.

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