新型耐热植酸酶对断奶仔猪生长性能与小肠黏膜形态结构的影响

曹贵彬, 于会民, 游金明, 韦启鹏, 邹志恒, 辛向荣

doi:10.3969/j.issn.1006-267x.2014.09.045

动物营养学报 >

2014 , Vol. 26 >Issue 9: 2814 - 2821

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.09.045

研究简报 Short Communications

新型耐热植酸酶对断奶仔猪生长性能与小肠黏膜形态结构的影响

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1. 江西农业大学动物科技学院, 南昌 330045;
2. 中国农业科学院饲料研究所, 北京 100081;
3. 江西省农业科学院畜牧研究所, 南昌 330200

曹贵彬（1989-），男，江西龙南人，硕士研究生，研究方向为动物营养与饲料。E-mail：839419233@qq.com

收稿日期: 2014-03-11

网络出版日期: 2014-09-10

基金资助

“十二五”农村领域国家科技计划（2013BAD10B01）；“十二五”国家科技支撑计划（2011BAD26B03）；农业部“948”项目（2011-G7）

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Effects of New Heat-Resistant Phytase on Growth Performance and Small Intestinal Mucosa Morphology Structure of Weaner Piglets

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1. College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China;
2. Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
3. Institute of Animal Science, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China

Received date: 2014-03-11

Online published: 2014-09-10

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摘要

本研究旨在探讨低磷饲粮中添加新型耐热植酸酶对断奶仔猪生长性能与小肠黏膜形态结构的影响。试验选用256头遗传背景相同、初始体重为（8.37±0.97） kg的30日龄断奶仔猪，按照完全随机区组的方法分为4个处理，每个处理设4个重复，每个重复16头仔猪。4个处理分别饲喂基础饲粮、低磷饲粮、低磷饲粮+500 U/kg新型耐热植酸酶及低磷饲粮+1 000 U/kg新型耐热植酸酶。试验期为30 d。结果表明：1）2个新型耐热植酸酶添加组相比较低磷饲粮组可提高仔猪平均日增重并降低料重比，但差异不显著（P>0.05）；2）1 000 U/kg新型耐热植酸酶添加组空肠和回肠绒毛高度、500 U/kg新型耐热植酸酶添加组回肠绒毛高度均显著高于低磷饲粮组（P<0.05），1 000 U/kg新型耐热植酸酶添加组十二指肠隐窝深度显著低于基础饲粮组和低磷饲粮组（P<0.05），1 000 U/kg新型耐热植酸酶添加组十二指肠绒毛高度与隐窝深度比值显著高于低磷饲粮组（P<0.05）。由此可知，断奶仔猪饲粮中添加新型耐热植酸酶用以减少无机磷的添加，能达到与基础饲粮组仔猪生长性能相近的效果，且对小肠黏膜形态结构有促进作用；添加新型耐热型植酸酶1 000 U/kg优于添加500 U/kg的效果。

关键词： 新型耐热植酸酶; 仔猪; 生长性能; 小肠黏膜形态; 无机磷

本文引用格式

曹贵彬, 于会民, 游金明, 韦启鹏, 邹志恒, 辛向荣 . 新型耐热植酸酶对断奶仔猪生长性能与小肠黏膜形态结构的影响[J]. 动物营养学报, 2014 , 26(9) : 2814 -2821 . DOI: 10.3969/j.issn.1006-267x.2014.09.045

Abstract

This experiment was conducted to study the effects of new heat-resistant phytase (NHP) in the low phosphors diet on growth performance and small intestinal mucosa morphology structure of weaner piglets. A total of 256 piglets weaned at 30 days of age with the same genetic background and with the body weight of (8.37±0.97) kg were randomly allotted to 4 treatments with 4 replicates of 16 piglets using a randomized complete-block design. They were fed a basal diet, a low phosphorus diet, the low phosphorus diet+500 U/kg NHP and the low phosphorus diet+1 000 U/kg NHP, respectively. The experiment lasted for 30 d. The results showed as follows: 1) compared with low phosphorus diet, supplementation of 500 U/kg and 1 000 U/kg NHP improved average daily gain and decreased the feed/gain (P>0.05). 2) Compared with the low phosphorus diet group, ileum villus height in 500 U/kg NHP group, jejunum and ileum villus height in 1 000 U/kg NHP group were significantly increased (P<0.05), duodenum crypt depth in 1 000 U/kg NHP group was significantly decreased (P<0.05), and duodenum villus height/crypt in 1 000 U/kg NHP group was significantly increased (P<0.05). In conclusion, using NPH to reduce part of phosphors in weaner piglets' diet can reach the similar effect of basal diet on the growth performance, and can promote mucosal structure of small intestine of piglets to some degree. In addition, the supplement effect of NHP at 1 000 U/kg is better than that at 500 U/kg.

Key words： new heat-resistant phytase; piglets; growth performance; small intestinal mucosa morphology; inorganic phosphorus

参考文献

[1] 蒋宗勇,林映才,姜文联,等.仔猪有效磷需要量的研究[J].动物营养学报,1999,11(1):44-50.

[2] 李光霞,李宗伟.植酸酶的应用及研究进展[J].畜牧与饲料科学,2009,30(3):61-63.

[3] 贺建华.植酸磷和植酸酶研究进展[J].动物营养学报,2005,17(1):1-6.

[4] KOPKO I I,KYRYLIV I I,STOIANOVS'KA H M.Effect of exogenous factors on metabolism of phosphorous compounds in the chicken tissues[J].Ukrainskiǐ Biokhimicheskiǐ Zhurnal,2003,75(5):90-94.

[5] 傅启高.植酸对单胃动物的抗营养作用[J].动物营养学报,1998,10(4):1-10.

[6] 蒋守群,吴天星.植酸酶的研究进展[J].动物营养学报,1999,11(3):1-11.

[7] KELLY S M,HUNTER J O.Epidermal growth factor stimulates synthesis and secretion of mucus glycoproteins in human gastric mucosa[J].Clinical Science,1990,79(5):425-427.

[8] ANGEL R,TAMIM N M,APPLEGATE T J,et al.Phytic acid chemistry:influence on phytin-phosphorus availability and phytase efficacy[J].The Journal of Applied Poultry Research,2002,11(4):471-480.

[9] RAVINDRAN V,HEW L I,RAVINDRAN G,et al.Influence of xylanase supplementation on the apparent metabolizable energy and ileal amino acid digestibility in a diet containing wheat and oats,and on the performance of three strains of broiler chickens[J].Australian Journal of Agricultural Research,1999,50(7):1159-1163.

[10] DENBOW D M,RAVINDRAN V,KORNEGAY E T,et al.Improving phosphorus availability in soybean meal for broilers by supplemental phytast[J].Poultry Science,1995,74(11):1831-1842.

[11] MITCHELL R D,EDWARDS H M,Jr.Effects of phytase and 1,25-dihydroxycholecalciferol on phytate utilization and the quantitative requirement for calcium and phosphorus in young broiler chickens[J].Poultry Science,1996,75(1):95-110.

[12] HATTEN L F,Ⅲ,INGRAM D R,PITTMAN S T.Effect of phytase on production parameters and nutrient availability in broilers and laying hens:a review[J].The Journal of Applied Poultry Research,2001,10(3):274-278.

[13] 董国忠,周安国,杨凤,等.饲粮蛋白质水平对早期断奶仔猪大肠蛋白质腐败作用和腹泻的影响[J].畜牧兽医学报,1996,27(4):293-302.

[14] HAMILTON D L,ROE W E.Electrolyte levels and net fluid and electrolyte movements in the gastrointestinal tract of weanling swine[J].Canadian Journal of Comparative Medicine,1977,41(3):241-250.

[15] YI Z,KORNEGAY E T,RAVINDRAN V,et al.Improving phytate phosphorus availability in corn and soybean meal for broilers using microbial phytase and calculation of phosphorus equivalency values for phytase[J].Poultry Science,1996,75(2):240-249.

[16] 赵春,朱忠珂,李勤凡,等.不同剂型植酸酶对肉仔鸡生长性能和钙、磷利用率的影响[J].动物营养学报,2007,19(6):714-718.

[17] 刘自逵,黄兴国,刘文敏,等.不同植酸酶对生长育肥猪生产性能及养分利用的影响[J].中国畜牧兽医,2010,37(2):22-26.

[18] YOUNG L G,LEUNISSEN M,ATKINSON J L.Addition of microbial phytase to diets of young pigs[J].Journal of Animal Science,1993,71(8):2147-2150.

[19] 孙宏选,高秀华,杨禄良.玉米-豆粕型日粮中添加高剂量植酸酶对肉鸡生长性能和血清生化指标的影响[J].中国畜牧兽医,2011,38(4):11-15.

[20] 黄兴国,王超,杨承剑,等.不同植酸酶对饲喂低磷日粮肉鸡生长性能及粪N、P排泄的影响[J].中国畜牧兽医,2008,35(7):25-28.

[21] 蔡青和,计成,岳洪源.玉米豆粕型日粮中添加植酸酶对断奶仔猪生产性能、养分消化率及血清生化指标的影响[J].动物营养学报,2004,16(2):15-21.

[22] NOURMOHAMMADI R,HOSSEINI S M,FARHANGFAR H.Effect of citric acid and microbial phytase on serum enzyme activities and plasma minerals retention in broiler chicks[J].African Journal of Biotechnology,2011,10(62):13640-13650.

[23] 胡骁飞.生长猪应用植酸酶日粮适宜钙磷比的研究[D].硕士学位论文.郑州:河南农业大学,2005.

[24] 李青竹,张立春,郑艳秋,等.吉林白鹅小肠形态学发育规律[J].中国兽医学报,2011,31(4):565-568.

[25] TANG M,LAARVELD B,VAN KESSEL A G,et al.Effect of segregated early weaning on postweaning small intestinal development in pigs[J].Journal of Animal Scienee,1999,77(12):3191-3200.

[26] 沈水宝,冯定远.外源酶对仔猪消化道食糜pH值和小肠绒毛形态结构的影响//中国畜牧兽医学会动物营养学分会--第九届学术研讨会论文集.北京:中国畜牧兽医学会,2004:56.

[27] 游金明,王自蕊,瞿明仁,等.大豆抗原蛋白的生物学特性及其对仔猪的过敏反应[J].饲料工业,2007,28(19):53-56.

[28] 邱梅平.不同剂量及不同剂型植酸酶对蛋鸡生长性能及养分利用率的影响[D].硕士学位论文.南京:南京农业大学,2009.

[29] 谢南彬,冯琳,刘扬,等.幼建鲤磷缺乏的组织病理观察[J].动物营养学报,2010,22(5):1328-1333.

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