动物营养学报 >

2014 , Vol. 26 >Issue 11: 3356 - 3361

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.11.023

分子营养 Molecular Nutrition

脂多糖刺激对仔猪下丘脑-垂体-肾上腺轴Toll样受体4信号通路关键基因表达的影响

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  • 武汉轻工大学动物营养与饲料科学湖北省重点实验室, 武汉 430023
陈少魁(1989- ),男,湖北荆州人,硕士研究生,从事猪营养生理机能调控的研究。E-mail:loveskchen@outlook.com

收稿日期: 2014-05-05

  网络出版日期: 2014-11-17

基金资助

国家自然科学基金面上项目(31172222,31372318);湖北省自然科学基金杰出青年人才项目(2013CFA029);湖北省国际科技合作项目(2011BFA008)

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Effects of Lipopolysaccharide Challenge on Key Gene Expression in Toll-Like Receptor 4 Signaling Pathway of Hypothalamic-Pituitary-Adrenal Axis of Piglets

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  • Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China

Received date: 2014-05-05

  Online published: 2014-11-17

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摘要

本试验旨在研究脂多糖(LPS)刺激对仔猪下丘脑-垂体-肾上腺(HPA)轴Toll样受体4(TLR4)信号通路关键基因表达的影响。选取12头断奶仔猪,分成2个组,每个组6个重复。试验组注射100 μg/kg体重的LPS,对照组注射等量的生理盐水。注射LPS或生理盐水4 h后采血,测定血浆中与应激相关激素的含量;采血后屠宰仔猪,取下丘脑、垂体、肾上腺,测定TLR4信号通路关键基因的mRNA表达水平,包括TLR4、髓样分化因子88(MyD88)、白细胞介素-1受体相关激酶1(IRAK1)、肿瘤坏死因子受体相关因子6(TRAF6)和核转录因子-κB(NF-κB)mRNA表达水平。结果表明:与对照组相比,1)LPS刺激导致血浆肿瘤坏死因子-α、皮质醇和促肾上腺皮质激素含量显著上升(P<0.05)。2)LPS刺激导致TLR4信号通路关键基因TLR4在下丘脑、垂体、肾上腺中mRNA表达水平显著升高(P<0.05);MyD88在垂体、肾上腺中mRNA表达水平显著升高(P<0.05),在下丘脑中mRNA表达水平有升高的趋势(P<0.10);IRAK1在垂体、肾上腺中mRNA表达水平有升高的趋势(P<0.10);TRAF6在下丘脑、垂体、肾上腺中mRNA表达水平显著升高(P<0.05);NF-κB在垂体中mRNA表达水平显著升高(P<0.05)。试验结果表明LPS刺激激活了HPA轴,诱导了HPA轴的TLR4信号通路关键基因的表达。

关键词： 仔猪; 脂多糖; HPA轴; TLR4信号通路

本文引用格式

陈少魁, 刘玉兰, 李权, 朱惠玲, 侯永清, 吴欢听, 王秀英, 刘称 . 脂多糖刺激对仔猪下丘脑-垂体-肾上腺轴Toll样受体4信号通路关键基因表达的影响[J]. 动物营养学报, 2014 , 26(11) : 3356 -3361 . DOI: 10.3969/j.issn.1006-267x.2014.11.023

Abstract

This experiment was conducted to investigate the effects of lipopolysaccharide (LPS) challenge on the expression of the key genes in toll-like receptor 4 (TLR4) signaling pathway of hypothalamic-pituitary-adrenal (HPA) axis of piglets. Twelve weaner piglets were allotted to two groups (6 replicates per group and 1 piglet per replicate). The piglets in the experimental group were injected with 100 μg/kg body weight LPS, whereas the piglets in the control group were injected with an equivalent amount of sterile saline. At 4 h post-challenge, blood samples were collected for determining stress-related hormones contents in the plasma. Following blood collection at 4 h, the piglets were slaughtered, and hypothalamus, pituitary gland and adrenal gland were collected. The mRNA expression levels of the key genes in TLR4 signaling pathway, including TLR4, myeloid differentiation factor 88 (MyD88), IL-1 receptor-associated kinase 1 (IRAK1), tumor necrosis factor receptor-associated factor 6 (TRAF6) and nuclear factor-κB (NF-κB) in HPA axis, were detected by real-time quantitative PCR. The results showed as follows: compared with control group, 1) LPS significantly increased plasma TNF-α, cortisol and adrenocorticotropin contents (P<0.05). 2)After LPS challenge, TLR4 mRNA expression level was significantly increased in hypothalamus, pituitary gland and adrenal gland (P<0.05). MyD88 mRNA expression level was significantly increased in pituitary gland and adrenal gland (P<0.05). The mRNA expression level of MyD88 in the hypothalamus had a tendency to increase (P<0.10). IRAK1 mRNA expression level in pituitary gland and adrenal gland had a tendency to increase (P<0.10). TRAF6 mRNA expression level was significantly increased in hypothalamus, pituitary gland and adrenal gland (P<0.05). NF-κB mRNA expression level was significantly increased in pituitary gland (P<0.05). These results indicate that LPS challenge actives HPA axis and induces the mRNA expression levels of the key genes in TLR4 signaling pathway of HPA axis.

Key words： piglets; LPS; HPA axis; TLR4 signaling pathway

参考文献

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