饲料中添加甘露寡糖对团头鲂幼鱼生长性能、抗氧化能力和肠道菌群的影响

孙盛明, 谢骏, 朱健, 戈贤平, 江晓浚

doi:10.3969/j.issn.1006-267x.2014.11.025

动物营养学报 >

2014 , Vol. 26 >Issue 11: 3371 - 3379

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.11.025

饲料营养 Feed Science and Technology

饲料中添加甘露寡糖对团头鲂幼鱼生长性能、抗氧化能力和肠道菌群的影响

展开

1. 中国水产科学研究院珠江水产研究所, 农业部热带亚热带水产资源利用与养殖重点实验室, 广州 510380;
2. 中国水产科学研究院淡水渔业研究中心, 农业部淡水渔业和种质资源利用重点实验室, 无锡 214081;
3. 南京农业大学无锡渔业学院, 无锡 214081

孙盛明(1983- ),男,山东莱州人,助理研究员,博士,研究方向为水产动物营养与饲料研究。E-mail:sunsm@ffrc.cn

收稿日期: 2014-05-04

网络出版日期: 2014-11-17

基金资助

农业部热带亚热带水产资源利用与养殖重点实验室开放基金课题(5-14001);国家大宗淡水鱼类产业技术体系华东养殖岗位(CARS-46-14);十二五国家科技支撑计划长江下游池塘高效生态养殖技术集成与示范(2012BAD25B07)

收起

Effects of Dietary Mannan Oligosaccharides on Growth Performance, Antioxidant Ability and Intestinal Microflora of Juvenile Blunt Anout Bream (Megalobrama amblycephala)

Expand

1. Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China;
2. Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China;
3. WuXi Fishery College, Nanjing Agricultural University, Wuxi 214081, China

Received date: 2014-05-04

Online published: 2014-11-17

Fold

摘要

本文旨在研究饲料中添加甘露寡糖对团头鲂幼鱼生长性能、抗氧化能力和肠道菌群的影响。选用平均体重为(1.05±0.01) g的团头鲂幼鱼360尾,随机分为4组(每组3个重复,每个重复30尾),分别饲喂添加0(对照组)、100、200和400 mg/kg甘露寡糖的试验饲料,试验期为8周。结果表明:饲料中添加甘露寡糖对团头鲂幼鱼的增重率、饲料系数和成活率均无显著影响(P>0.05)。与对照组相比,饲料中添加100、200和400 mg/kg甘露寡糖显著提高了肝脏超氧化物歧化酶和过氧化氢酶活性(P<0.05),显著降低了肝脏丙二醛含量(P<0.05)。变性梯度凝胶电泳(DGGE)指纹图谱结果分析表明,200和400 mg/kg甘露寡糖组团头鲂幼鱼肠道菌群Shannon-Wiener指数显著低于对照组(P<0.05),其优势菌主要为放线菌门(Actinobacteria)、变形菌门(Proteobacteria)和硬壁菌门(Firmicutes)。由此可见,饲料中添加200~400 mg/kg甘露寡糖能提高团头鲂幼鱼的抗氧化能力,改善肠道菌群结构。

关键词： 团头鲂; 甘露寡糖; 生长性能; 抗氧化能力; 肠道菌群

本文引用格式

孙盛明, 谢骏, 朱健, 戈贤平, 江晓浚 . 饲料中添加甘露寡糖对团头鲂幼鱼生长性能、抗氧化能力和肠道菌群的影响[J]. 动物营养学报, 2014 , 26(11) : 3371 -3379 . DOI: 10.3969/j.issn.1006-267x.2014.11.025

Abstract

This experiment was conducted to investigate the effects of dietary mannose oliogosaccharides (MOS) on growth performance, antioxidant ability and intestinal microflora of juvenile blunt anout bream (Megalobrama amblycephala). A total of 360 juvenile blunt anout bream with the average body weight of (1.05±0.01) g were randomly divided into 4 groups with 3 replicates per group and 30 fish per replicate. The fish in control group were fed a basal diet, and the fish in MOS supplemental groups were fed the experimental diets supplemmented with 100, 200 and 400 mg/kg MOS on the basis of basal diet, respectively. After 8 weeks feeding, the results showed as follows: there were no significantly differences in weight gain rate, feed conversion ratio and survival rate among all groups (P>0.05). Compared with control group, supplementation with 100, 200 and 400 mg/kg MOS significantly improved the liver superoxide dismutase and catalase activities (P<0.05), while significantly decreased the liver malonaldehyde content (P<0.05). Furthermore, the denatured gradient gel electrophoresis (DGGE) fingerprints demonstrated that the Shannon-Wiener index of intestinal microflora in 200 and 400 mg/kg MOS groups was significantly lower than that in control group (P<0.05), and the main predominant bacteria in 200 and 400 mg/kg MOS groups were Actinobacteria, Proteobacteria and Firmicutes. It is concluded that supplementation with 200 to 400 mg/kg MOS can elevate the antioxidant ability and improve the intestinal microflora structure of juvenile blunt anout bream.

Key words： blunt anout bream (Megalobrama amblycephala); mannose oligosaccharides; growth performance; antioxidant ability; intestinal microflora

参考文献

[1] 吴淑勤,王亚军.我国水产养殖病害控制技术现状与发展趋势[J].中国水产,2010(8):9-10.

[2] SIMS M D,DAWSON K A,NEWMAN K E,et al.Effects of dietary mannan oligosaccharide,bacitracin methylene disalicylate,or both on the live performance and intestinal microbiology of turkeys[J].Poultry Science,2004,83(7):1148-1154.

[3] ROZEBOOM D W,SHAW D T,TEMPELMAN R J,et al.Effects of mannan oligosaccharide and an antimicrobial product in nursery diets on performance of pigs reared on three different farms[J].Journal of Animal Science,2005,83(11):2637-2644.

[4] 明建华,谢骏,徐跑,等.团头鲂(Megalobrama amblycephala)两种热休克蛋白70(HSP70s)的原核表达、纯化及鉴定[J].海洋与湖沼,2012,43(1):185-191.

[5] 崔红红,刘波,戈贤平,等.肌醇对氨氮应激下团头鲂幼鱼免疫的影响[J].水产学报,2014,38(2):228-236.

[6] 徐磊,刘波,谢骏,等.甘露寡糖对异育银鲫生长性能、免疫及HSP70基因表达的影响[J].水生生物学报,2012,36(4):656-663.

[7] AKRAMI R,RAEZGHI-MANSOUR M,CHITSAZ H,et al.Effect of dietary mannan oligosaccharide on growth performance,survival,body composition and some hematological parameters of carp juvenile (Cyprinus carpio)[J].Journal of Animal Science Advances,2012,2(11):879-885.

[8] STAYKOV Y,SPRING P,DENEV S,et al.Effect of a mannan oligosaccharide on the growth performance and immune status of rainbow trout (Oncorhynchus mykiss)[J].Aquaculture International,2007,15(2):153-161.

[9] DIMITROGLOU A,MERRIFIELD D L,SPING P,et al.Effects of mannan oligosaccharide (MOS) supplementation on growth performance,feed utilisation,intestinal histology and gut microbiota of gilthead sea bream (Sparus aurata)[J].Aquaculture,2010,300(1/2/3/4):182-188.

[10] 李晓,李冰,董玉峰,等.精养团头鲂池塘沉积物微生物群落的结构特征及组成多样性分析[J].水产学报,2014,38(2):218-227.

[11] DIMITROGLOU A,MERRIFIELD D L,MOATE R,et al.Dietary mannan oligosaccharide supplementation modulates intestinal microbial ecology and improves gut morphology of rainbow trout,Oncorhynchus mykiss (Walbaum)[J].Journal of Animal Science,2009,87(10):3226-3234.

[12] GENC M A,AKTAS M,GENC E,et al.Effects of dietary mannan oligosaccharide on growth,body composition and hepatopancreas histology of Penaeus semisulcatus (de Haan 1844)[J].Aquaculture Nutrition,2007,13(2):156-161.

[13] DANIELS C L,MERRIFIELD D L,BOOTHROYD D P,et al.Effect of dietary Bacillus spp.and mannan oligosaccharides (MOS) on European lobster (Homarus gammarus L.) larvae growth performance,gut morphology and gut microbiota[J].Aquaculture,2010,304(1/2/3/4):49-57.

[14] GRISDALE-HELLAND B,HELLAND S J,GATLIN Ⅲ D M,et al.The effects of dietary supplementation with mannanoligosaccharide,fructooligosaccharide or galactooligosaccharide on the growth and feed utilization of Atlantic salmon (Salmo salar)[J].Aquaculture,2008,283(1/2/3/4):163-167.

[15] 王红宁.鲤肠道正常菌群的研究[J].水生生物学报,1994,18(4):354-359.

[16] DAVIS M E,MAXWELL C V,BROWN D C,et al.Effect of dietary mannan oligosaccharides and(or) pharmacological additions of copper sulfate on growth performance and immunocompetence of weanling and growing/finishing pigs[J].Journal of Animal Science,2002,80(11):2887-2894.

[17] GU M,MA H M,MAI K S,et al.Effects of dietary β-glucan,mannanoligosaccharide and their combinations on growth performance,immunity and resistance against Vibrio splendidus of sea cucumber,Apostichopus japonicus[J].Fish & Shellfish Immunology,2011,31(2):303-309.

[18] TORRECILLAS S,MAKOL A,CABALLERO M J,et al.Immune stimulation and improved infection resistance in European sea bass (Dicentrarchus labrax) fed mannan oligosaccharides[J].Fish & Shellfish Immunology,2007,23(5):969-981.

[19] SANG H M,FOTEDAKAR R,FILER K.Effects of dietary mannan oligosaccharide on the survival,growth,immunity and digestive enzyme activity of freshwater crayfish,Cherax destructor Clark (1936)[J].Aquaculture Nutrition,2011,17(2):e629-e635.

[20] 王锐,刘军,刘辉宇.半乳甘露寡糖对异育银鲫幼鱼生长和非特异性免疫的影响[J].上海水产大学学报,2008,17(4):502-506.

[21] 刘爱君,冷向军,李小勤,等.甘露寡糖对奥尼罗非鱼(Oreochromis niloticus×O.aureus)生长、肠道结构和非特异性免疫的影响[J].浙江大学学报:农业与生命科学版,2009,35(3):329-336.

[22] 于艳梅,吴志新,陈孝煊,等.魔芋甘露寡糖对黄颡鱼非特异性免疫功能及生长的影响[J].华中农业大学学报,2010,29(3):351-355.

[23] 姚延丹,李谷,陶玲,等.复合人工湿地-池塘养殖生态系统细菌多样性研究[J].环境科学与技术,2011,34(7):51-55.

[24] 马相杰,汪立平,赵勇,等.甘露寡糖对罗非鱼幼鱼肠道微生物的影响[J].微生物学通报,2010,37(5):708-713.

[25] 吕慧源,周志刚.饲喂果寡糖对奥尼罗非鱼Oreochromisaurea ♂×O.nilotica♀肠道菌群、成活率及生长性能的影响[J].动物营养学报,2007,19(6):691-697.

[26] 杜英男,鞠贵春,薛军,等.果聚糖和甘露寡糖对水貂肠道菌群影响的研究[J].经济动物学报,2007,11(2):83-86.

[27] YANG G M,BAO B L,PEATMAN E,et al.Analysis of the composition of the bacterial community in puffer fish Takifugu obscurus[J].Aquaculture,2007,262(2/3/4):183-191.

[28] KIM D H,BRUNT J,AUSTIN B.Microbial diversity of intestinal contents and mucus in rainbow trout (Oncorhynchus mykiss)[J].Journal of Applied Microbiology,2007,102(6):1654-1664.

Options

文章导航

摘要

本文引用格式

Abstract

参考文献

关于本刊

在线期刊

作者中心

相关单位

联系我们

模态框（Modal）标题

摘要

本文引用格式

Abstract

参考文献

关于本刊

在线期刊

作者中心

相关单位

联系我们