动物营养学报 >

2014 , Vol. 26 >Issue 11: 3404 - 3413

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.11.029

研究简报 Short Communications

生长猪回肠末端瘘管中食糜养分流量及指示剂回收率的变异

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  • 1. 中国农业科学院北京畜牧兽医研究所, 动物营养学国家重点实验室, 北京 100193;
    2. 华南农业大学动物科学学院, 广州 510642
刘成玲(1990- ),男,江西宜春人,硕士研究生,从事饲料营养价值评定。E-mail:13141465205@163.com

收稿日期: 2014-05-01

  网络出版日期: 2014-11-17

基金资助

国家自然科学基金项目(31172215);科技部创新方法工作专项(2009IM033100);中国农业科学院科技创新工程(ASTIP-IAS07)

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Variation of Nutrients Flow and Recovery Rate of Inert Marker in Digesta Collected with T-Canuula at the Terminal Ileum of Growing Pigs

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  • 1. State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
    2. College of Animal Science, South China Agriculture University, Guangzhou 510642, China

Received date: 2014-05-01

  Online published: 2014-11-17

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摘要

食糜样品的代表性是研究养分在猪体内消化过程的关键。通过T型瘘管获得的代表性食糜采样方案必须基于瘘管中食糜养分的流量及指示剂的回收率。本试验以4头在回肠末端安装瘘管的生长猪[(21.3±1.0) kg]为试验对象,饲喂玉米-豆粕型饲粮。采用3×16双因素完全随机设计,其中采样日设3个水平,即试验期第8、9、10天。日内采样时间设16个水平,即08:00~24:00,每小时通过瘘管采集1次回肠食糜样品,记录鲜食糜的重量并分析食糜样品中总能,干物质、粗蛋白质及三氧化二铬的含量。研究结果如下:1)双因素方差分析表明,日内采样时间显著地影响了瘘管中食糜的鲜食糜、干物质、总能、粗蛋白质和三氧化二铬的流量(P<0.05);在采样日间,瘘管中食糜的鲜食糜、干物质、粗蛋白质和三氧化二铬的日内平均流量均无显著性差异(P>0.05),但回肠末端食糜的总能流量有显著性差异(P<0.05)。2)日内16 h累计流经回肠瘘管的食糜及全天流经回肠末端的食糜在干物质、总能、粗蛋白质总流量的日间变异上有显著性差异(P<0.05),其中试验期第8天的流量均显著地高于第9、10天的相应值(P<0.05),而第9、10天间流量无显著性差异(P>0.05)。通过瘘管连续收集16 h回肠末端食糜的各养分流量占全天流经回肠末端食糜相应养分总流量的比例都在68%以上,且不同采样日间无显著性差异(P>0.05)。3)通过回肠末端瘘管收集日内16 h食糜的三氧化二铬总流量及回收率在日间无显著性差异(P>0.05),平均回收率为72.9%,变异系数为6.87%。上述结果表明,在3 d的收集中,后面2 d回肠末端食糜养分的平均流量相对稳定。这2 d采食后8 h连续收集食糜有较好的代表性。建议3 d采样中,第1天作为采样预试期,后面2 d作为采样正试期。

关键词： 生长猪; 回肠末端; 食糜流量; 指示剂回收率; 变异

本文引用格式

刘成玲, 赵峰, 左建军, 王钰明, 张莉, 高理想, 张宏福 . 生长猪回肠末端瘘管中食糜养分流量及指示剂回收率的变异[J]. 动物营养学报, 2014 , 26(11) : 3404 -3413 . DOI: 10.3969/j.issn.1006-267x.2014.11.029

Abstract

Representation of digesta samples is crucial to study on the in vivo digestion process of dietary nutrients in pigs, thereby, sampling scheme for representative digesta has to be based on the nutrients flow and the recovery of inert marker in digesta collected with T-cannula in pigs. In current experiment, 4 growing [(21.3±1.0) kg] pigs fitted with T-cannulas at the terminal ileum were fed with a corn-soybean meal diet in individual metabolic crate. Three digesta collection days for the 8th, 9th, 10th and 16th hourly digesta collection time from 08:00 to 24:00 per day were used in a 3×16 factorial arrangement. Hourly digesta was collected and weighed, then analyzed for gross energy and dry matter, crude protein and chromic oxide (Cr2O3) content. The results showed as follows: 1) using two-way ANOVA, collection time significantly affected the flow of fresh digesta, dry matter, gross energy, crude protein and Cr2O3 in cannula (P<0.05). No significant differences were observed for the daily mean flow in each of fresh digesta, dry matter, crude protein and Cr2O3 among 3 collection days (P>0.05), whereas, flow of gross energy was excluded (P<0.05). 2) The collection day significantly affected the accumulative flow of dry matter, gross energy, crude protein for 16 h in cannula and 24 h at terminal ileum (P<0.05). The both of accumulative flow in the 8th day of experiment period were greater than these in the 9th and 10th days (P<0.05), whereas no significant difference was observed for the 9th and 10th days (P>0.05). The accumulative flow of each nutrient for 16 h in T-cannula was greater than 68.0% of that passed the intestine in 24 h at terminal ileum. No significant difference was observed for this percentage among collection days (P>0.05).3) No significant daily difference was observed for the Cr2O3 flow collected for 16 h with T-cannula and the recovery rate of Cr2O3, respectively(P>0.05). The mean and coefficient of variation (CV) of recovery rate of Cr2O3 was 72.9% and 6.87%, respectively. In conclusion, during 3 days collection, a stable nutrients flow at terminal ileum is present in the last 2 days. Two continuous 8 hours collection after feeding can get satisfactory representative digesta sample. In a 3-day-collection period, the first day and following 2 days are suggested to be adaption and regular collection of digesta samples, respectively.

Key words： growing pigs; terminal ileum; digesta flow; recovery rate of index; variation

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