酵母培养物水溶物对离体草鱼肠道黏膜细胞生长及细胞膜完整性的影响

姚仕彬, 叶元土, 蔡春芳, 张宝彤, 董娇娇, 黄雨薇, 陈科全

doi:10.3969/j.issn.1006-267x.2014.11.038

动物营养学报 >

2014 , Vol. 26 >Issue 11: 3478 - 3484

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.11.038

研究简报 Short Communications

酵母培养物水溶物对离体草鱼肠道黏膜细胞生长及细胞膜完整性的影响

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1. 苏州大学基础医学与生物科学学院, 江苏省水产动物营养重点试验室, 苏州 215123;
2. 北京市营养源研究所系统营养工程技术研究中心, 北京 100069

姚仕彬(1986- ),男,四川成都人,硕士研究生,从事水产动物营养与饲料研究。E-mail:yaoshibin2006@163.com

收稿日期: 2014-05-14

网络出版日期: 2014-11-17

基金资助

国家自然科学基金项目(31172417);苏州市应用基础(农业)项目(N313401210)

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Effects of Water Soluble Material of Yeast Culture on Growth and Cell Membrane Integrity of Intestinal Epithelial Cells in Vitro of Grass Carp (Ctenopharyngodon idella)

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1. Key Laboratory of Aquatic Nutrition of Jiangsu Province, School of Biology and Basic Medical Sciences, Suzhou University, Suzhou 215123, China;
2. Open Laboratory for Aquatic Animal Nutrition, Beijing Research Institute for Nutritional Resources, Beijing 100069, China

Received date: 2014-05-14

Online published: 2014-11-17

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摘要

本试验以酵母培养物水溶物为试验材料,添加于离体草鱼肠道黏膜细胞培养液中,研究酵母培养物水溶物在不同浓度、不同作用时间下对细胞生长及细胞膜完整性的影响。采用单因子试验设计,设1个对照组及5个酵母培养物水溶物组(YC-1~5组),各组均设96个重复,每个重复为1个培养孔。对照组的培养液中不添加酵母培养物水溶物,YC-1~5组的培养液中酵母培养物水溶物的浓度分别为10、25、50、100、200 mg/L。结果表明:培养液中添加50~200 mg/L酵母培养物水溶物对细胞形态无损伤,100~200 mg/L酵母培养物水溶物在添加后3 h时显著增强细胞活性(P<0.05),50 mg/L酵母培养物水溶物在添加后6 h时显著增强细胞活性(P<0.05)。与对照组相比,3 h时各酵母培养物水溶物组培养液中乳酶脱氢酶(LDH)活力均没有显著变化(P>0.05),6 h时YC-4、YC-5组显著降低(P<0.05),9 h时YC-3、YC-4、YC-5组显著降低(P<0.05),12 h时YC-2、YC-3、YC-4、YC-5组显著降低(P<0.05)。各时间点(3、6、9、12 h)各酵母培养物水溶物组培养液中谷丙转氨酶(GPT)、谷草转氨酶(GOT)活力与对照组相比均没有显著变化(P>0.05),但在6~9 h时GPT活力均低于对照组。由此得出,培养液中添加10~200 mg/L酵母培养物水溶物能促进离体草鱼肠道黏膜细胞的生长,保护细胞膜的完整性,其发挥保护作用的适宜浓度为50~200 mg/L。

关键词： 草鱼; 肠道黏膜细胞; 酵母培养物水溶物; 细胞生长; 细胞膜完整性

本文引用格式

姚仕彬, 叶元土, 蔡春芳, 张宝彤, 董娇娇, 黄雨薇, 陈科全 . 酵母培养物水溶物对离体草鱼肠道黏膜细胞生长及细胞膜完整性的影响[J]. 动物营养学报, 2014 , 26(11) : 3478 -3484 . DOI: 10.3969/j.issn.1006-267x.2014.11.038

Abstract

In this experiment, water soluble material of yeast culture as the experimental material was added in the culture solution of intestinal epithelial cells in vitro of grass carp (Ctenopharyngodon idella), in order to study the effects of water soluble material of yeast culture on growth and cell membrane integrity under the different concentrations and different action time of water soluble material of yeast culture. One control group and 5 water soluble material of yeast culture groups (YC-1 to 5 groups) were designed by using single-factor experimental design, and each group had 96 replicates and 1 replicate was a culture hole. The control group was not added the water soluble material of yeast culture, and the concentration of water soluble material of yeast culture in YC-1 to 5 groups was 10, 25, 50, 100 and 200 mg/L, respectively. The results showed as follows: the cell morphology was no injury when adding 50 to 200 mg/L water soluble material of yeast culture into the culture solution, the cell activity was increased on 3 h after added 100 to 200 mg/L water soluble material of yeast culture, and the cell activity was increased on 6 h after added 50 mg/L water soluble material of yeast culture. Compared with control group, the activity of lactate dehydrogenase (LDH) in culture solution in all water soluble material of yeast culture groups had no significant change on 3 h (P>0.05), while that in YC-4 and YC-5 groups was significantly reduced on 6 h (P<0.05), that in YC-3, YC-4 and YC-5 groups was significantly reduced on 9 h (P<0.05), and that in YC-2, YC-3, YC-4 and YC-5 groups was significantly reduced on 12 h (P<0.05). The activities of glutamic-pyruvic transaminase (GPT) and glutamic-oxaloacetic transaminase (GOT) in culture solution in all water soluble material of yeast culture groups had no significant change on all time points (3, 6, 9 and 12 h) compared with control group (P>0.05), but the activity of GOP on 6 to 9 h in all water soluble material of yeast culture groups was lower than that in control group. In conclusion, adding 10 to 200 mg/L water soluble material of yeast culture into the culture solution can improve the growth and protect the cell membrane integrity of intestinal epithelial cells in vitro of grass carp, and the appropriate concentration of playing protective effect is 50 to 200 mg/L.

Key words： grass carp (Ctenopharyngodon idella); intestinal epithelial cells; water soluble material of yeast culture; cell growth; cell membrane integrity

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