本试验旨在研究葡萄糖氧化酶(GOD)缓解霉变饲料对小鼠平均日增重和肠道菌群变化的影响。18~22 g昆明小鼠130只,随机分为13组。分别饲喂添加0、10%、20%、30%霉变玉米面和0、0.1%、0.3%、0.5% GOD的饲料,试验期为60 d。结果表明:1)不同霉变玉米添加量的饲料饲喂小鼠60 d,与试验对照组比较,0.3% GOD能显著或极显著改善小鼠平均日增重(P<0.05或P<0.01)。2)添加10%霉变玉米面,0.3%和0.5% GOD组与试验对照组比较,极显著降低了大肠杆菌的数量(P<0.01),增加了乳酸杆菌数量(P<0.01);添加20%霉变玉米面,饲喂60 d时,3种不同浓度的GOD极显著降低了大肠杆菌数量(P<0.01),提高了乳酸杆菌数量(P<0.01);添加30%霉变玉米面,3种不同浓度的GOD显著或极显著减少了肠道大肠杆菌数量(P<0.05或P<0.01),0.3% GOD效果较好,饲喂60 d时,0.3%和0.5%的GOD添加量极显著提高了肠道乳酸杆菌数量(P<0.01)。结果提示,GOD能够缓解霉变饲料对小鼠生长造成的影响。饲料中黄曲霉毒素B₁含量低于67.77 μg/kg时,添加0.3%的GOD效果较好。

This paper was aimed to study the effects of glucose oxidase on average daily gain and intestinal flora in mice with moldy feed. A total of 130 KM mice, 18 to 22 g,were randomly assigned to 13 groups. The diet was added by 0, 10%, 20% and 30% moldy maize flour and 0, 0.1%, 0.3% and 0.5% GOD, respectively, and the experiment lasted for 60 days. The results showed as follows: compared with experimental control group, the average daily gain of mice fed with the diet added by moldy maize flour and 0.3% GOD for 60 days was significantly increased (P<0.05 or P<0.01). Addition of 0.3% and 0.5% GOD to the diet with 10% moldy maize flour significantly decreased the amount of bacterium E. coli (P<0.01), and increased the amount of Laetobacillus (P<0.01). When the mice were fed for 60 days with the diet added 10% moldy maize flour, GOD significantly decreased the amount of bacterium E. coli, and increased the amount of Laetobacillus (P<0.01). GOD decreased the amount of bacterium E. coli in mice fed with 30% moldy maize flour diet (P<0.05 or P<0.01), and 0.3% GOD was proper. Addition of 0.3% and 0.5% GOD increased the amount of Laetobacillus in mice fed for 60 days (P<0.01). This study demonstrates that GOD can ameliorate the effects of moldy diet on the growth of mice. Addition of 0.3% GOD is suggested on the condition that aflatoxin B₁ dose in diets is not more than 67.77 μg/kg.