陈香茶菌及枯草芽孢杆菌复合发酵豆粕的研究

姚勇芳, 赵祥杰, 廖延智, 陈小凤, 李平凡

doi:10.3969/j.issn.1006-267x.2014.11.046

动物营养学报 >

2014 , Vol. 26 >Issue 11: 3537 - 3544

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.11.046

研究简报 Short Communications

陈香茶菌及枯草芽孢杆菌复合发酵豆粕的研究

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1. 广东轻工职业技术学院食品系, 广州 510300;
2. 广东省农业科学院蚕业与农产品加工研究所, 广州 510610;
3. 韶关市粤凰生态科技有限公司, 韶关 512026

姚勇芳(1976- ),男,安徽安庆人,硕士研究生,研究方向为发酵饲料开发。E-mail:809833140@qq.com

收稿日期: 2014-05-16

网络出版日期: 2014-11-17

基金资助

广东省教育部产学研结合项目(2012B091100402);广东省星火计划项目(2012A020602095)

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Study on Mixed Fermentation of Soybean Meal By the Complex Flora from Crusted Tea and Bacillus subtilis

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1. Department of Food, Guangdong Industry Technical College, Guangzhou 510300, China;
2. Sericulture & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510610, China;
3. Shaoguan Yuehuang Ecological Technology Co. Ltd., Shaoguan 512026, China

Received date: 2014-05-16

Online published: 2014-11-17

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摘要

本试验旨在研究陈香茶菌及枯草芽孢杆菌复合发酵生料豆粕。以酸溶性蛋白含量为主要检测指标,研究陈香茶菌及枯草芽孢杆菌不同培养条件对豆粕固态发酵的影响。结果表明:1)陈香茶菌适宜培养条件为,称取0.5 g陈香茶粉接入装有100 mL马铃薯葡萄糖培养基三角瓶中,28 ℃静置培养24 h。2)豆粕固态发酵最佳工艺为,10%枯草芽孢杆菌发酵液+10%陈香茶菌培养液+10%糖蜜+10%水+55%豆粕,在37 ℃密闭条件下培养3~6 d。通过陈香茶菌及枯草芽孢杆菌复合发酵,能显著改善生料豆粕营养价值,有一定的应用价值。

关键词： 陈香茶菌; 枯草芽孢杆菌; 豆粕; 复合发酵

本文引用格式

姚勇芳, 赵祥杰, 廖延智, 陈小凤, 李平凡 . 陈香茶菌及枯草芽孢杆菌复合发酵豆粕的研究[J]. 动物营养学报, 2014 , 26(11) : 3537 -3544 . DOI: 10.3969/j.issn.1006-267x.2014.11.046

Abstract

The study was conducted to investigate the mixed fermentation of soybean meal by the complex flora from crusted tea and Bacillus subtilis. Making the acid soluble protein content as the main index, the different culture conditions of the complex flora from crusted tea and Bacillus subtilis fermentation factors were studied. The research results showed as follows: 1) the optimal culture conditions of the complex flora from crusted tea was: 0.5 g crusted tea powder into triangle bottle with 100 mL potato dextrose agar was stationarily cultured for 24 h under the condition of 28 ℃. 2) The optimum process for soybean meal by solid state fermentation was: 10% Bacillus subtilis fermentation liquid+10% culture liquid of the complex flora from crusted tea+10% molasses+10% water+55% soybean meal, at 37 ℃ under airtight condition cultured for 3 to 6 days. The mixed fermentation by the complex flora from crusted tea and Bacillus subtilis can significantly improve the nutritional value of raw meal and have certain application value.

Key words： the complex flora from crusted tea; Bacillus subtilis; soybean meal; mixed fermentation

参考文献

[1] 马文强,冯杰,刘欣.微生物发酵豆粕营养特性研究[J].中国粮油时报,2008,23(1):121-124.

[2] 雷晓燕.普洱茶中主要微生物的研究[J].沈阳化工学院学报,2009,23(2):134-137.

[3] 陆熹,李霞,仲小兰,等.微生物发酵在我国饲料工业中的应用及发展探讨[J].现代科技农业,2008,42(1):156-160.

[4] 吴胜华,李吕木,张邦辉,等.多菌种固态发酵豆粕生产小肽饲料[J].食品与发酵工业,2008,34(10):113-115.

[5] 魏炳栋,陈群,刘海燕,等.乳酸菌固态发酵对豆粕蛋白质含量、体外消化率及游离氨基酸含量的影响[J].饲料博览:技术版,2010,42(3):3-5.

[6] HU J K,LU W Q,WANG C L,et al.Characteristics of solid-state fermented feed and its effects on performance and nutrient digestibility in growing-finishing pigs[J].Asian-Australasian Journal of Animal Sciences,2008,21(11):1635-1641.

[7] 周静,肖嫩群.酵母菌与乳酸菌混合培养条件研究[J].安徽农业科学,2011,39(15):8824-8825.

[8] AGAR W T,HIRD F J R,SIDHU G S.The active absorption of amino acids by the intestine[J].The Journal of Physiology,1953,121(2):255-263.

[9] CHUNG S Y,SWAISGOOD H E,CATIGNANI G L.Effects of alkali treatment and treatment in the prence of fructose on digestibility of food proteins asdetermined by and immobilized digestive enzyme assay (IDEA)[J].Journal of Agricultural and Food Chemistry,1986,34(3):579-584.

[10] FEI Y J,KANAI Y,NUSSBERGER S,et al.Expression cloning of a mammalian proton-coupled oligopeptide transporter[J].Nature,1994,368(6471):563-566.

[11] HARA H,FUNABIKI R,IWATA M,et al.Portal absorption of small peptides in rats under unrestrained conditions[J].The Journal of Nutrition,1984,114(6):1122-1129.

[12] 贺月林,郭照辉,谭周进,等.酵母菌与乳酸菌混合培养中试研究[J].现代农业科技,2010,20(2):29-31.

[13] GABERT V M,SAUER W C,SCHMITZ M,et al.The effect of formic acid and buffering capacity on the ileal digestibilities of amino acids and bacterial populations and metabolites in the small intestine of weanling pigs fed semipurified fish meal diets[J].Canadian Journal of Animal Science,1995,75(4):615-623.

[14] BRODERICK G A,RADLOFF W J.Effect of molasses supplementation on the production of lactating dairy cows fed diets based on alfalfa and corn silage[J].Journal of Dairy Science,2004,87(9):2997-3009.

[15] 周伏忠,贾藴丽,陈国参,等.豆粕发酵适宜菌株筛选及其发酵技术研究[J].河南科学,2007,25(3):409-412.

[16] 莫重文,黄岗.固态发酵法生产发酵豆粕的研究[J].中国油脂,2007,32(7):38-40.

[17] 陆文清.发酵饲料生产与应用技术[M].北京:中国轻工业出版社,2011:99-100.

[18] 王金斌,马海乐,段玉清,等.混菌固态发酵豆粕生产优质高蛋白饲料研究[J].中国粮油学报,2011,24(2):120-124.

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