动物营养学报 >

2015 , Vol. 27 >Issue 3: 811 - 819

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.03.019

实验方法 Experimental Methods

猪生长阶段与饲粮类型对酸不溶灰分法测定养分消化率的影响

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  • 中国农业科学院北京畜牧兽医研究所, 动物营养学国家重点实验室, 北京 100193
王钰明(1991—),男,陕西咸阳人,硕士研究生,从事饲料营养价值的评定。E-mail:wudixiaoming@163.com

收稿日期: 2014-10-29

  网络出版日期: 2015-03-18

基金资助

国家自然科学基金项目(31172215);中国农业科学院科技创新工程(ASTIP-IAS07);科技部创新方法工作专项(2009IM033100)

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Effects of Growing Phase and Diet Type on Determination of Nutrient Digestibility by Acid Insoluble Ash Technique for Pigs

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  • State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese academy of Agriculture Science, Beijing 100193, China

Received date: 2014-10-29

  Online published: 2015-03-18

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摘要

本试验旨在探讨猪饲粮中添加硅藻土来源的盐酸不溶灰分(AIA)后,以饲粮和粪中AIA含量测定养分消化率的可行性。试验采用2×2两因素完全随机设计,其中饲粮设2个处理,即玉米-豆粕型饲粮和玉米-玉米干酒糟及其可溶物(cDDGS)-米糠粕型饲粮;猪生长阶段设2个处理,即生长期(60~100日龄)和育肥期(100~140日龄)。每个生长阶段分为2个试验期,每期试验包括5 d预试期、5 d全收粪期和3 d部分粪样定点采集期,测定饲粮及粪样的干物质、AIA、总能和粗蛋白质含量,从而计算饲粮的养分消化率及AIA的回收率。结果表明:1)玉米-豆粕型饲粮和玉米-cDDGS-米糠粕型饲粮中AIA的平均回收率分别为97.29%和97.54%,差异不显著(P>0.05);2)全收粪法和AIA法测定的养分消化率差异不显著(P>0.05),猪生长阶段和饲粮类型对其测定的养分消化率的绝对差影响不显著(P>0.05);3)部分粪样定点采集AIA法测定的干物质消化率与全收粪AIA法差异不显著(P>0.05),猪生长阶段和饲粮类型对其测定的干物质消化率的绝对差影响不显著(P>0.05)。综合以上研究结果,在2种猪饲粮中添加0.8%的硅藻土后,以AIA法测定饲粮养分消化率不受猪生长阶段及饲粮类型的影响,且AIA法与全收粪法的测值是可比的。

关键词： 硅藻土; 盐酸不溶灰分; 养分消化率

本文引用格式

王钰明, 赵峰, 陈寿飞, 孙永波, 张虎, 张宏福 . 猪生长阶段与饲粮类型对酸不溶灰分法测定养分消化率的影响[J]. 动物营养学报, 2015 , 27(3) : 811 -819 . DOI: 10.3969/j.issn.1006-267x.2015.03.019

Abstract

This experiment was conducted to validate the method for determination of nutrient digestibility according to the content of acid insoluble ash (AIA) in feces and diet added AIA from celite for pigs. Two diets [corn-soybean meal or corn-corn distillers dried grains with solubles (cDDGS)-rice bran meal diets] and two growing phases [growing period (60 to 100 days of age) or fattening period (100 to 140 days of age)] were used in a 2×2 factorial arrangement in a completely randomized design. Each phase contained 2 experimental periods with each consisted of 5 d preliminary feeding period, 5 d total faecal collection period and 3 d partial faecal collection period, respectively. The contents of dry matter, AIA, crude protein and gross energy in the diets and feces were determined for calculating the nutrient digestibility and the recovery of AIA. The results showed as follows: 1) there was no significant difference in the recovery of AIA in corn-soybean meal and corn-cDDGS-rice bran meal diets with 97.29% and 97.54%, respectively (P>0.05). 2) No significant difference were observed in the nutrient digestibility determined with total faecal collection and AIA technique (P>0.05). Growing phase of pigs and diet type had no significant influence on the absolute difference between nutrient digestibility determined with total faecal collection and AIA technique (P>0.05). 3) There was no significant difference in the dry matter digestibility (DMD) determined by the AIA content in the feces samples from partial faecal collection and total faecal collection (P>0.05). Growing phase of pigs and diet type had no significant influence on the absolute difference between the DMD determined with the content of AIA from these 2 sources (P>0.05). In conclusion, 0.8% celite added to the diet is satisfactory to obtain nutrient digestibility determined with the AIA content in diet and feces, and growing phase and diet type had no significant influence on the values determined with AIA technique.

Key words： celite; acid insoluble ash; nutrient digestibility

参考文献

[1] LANDERO J L,BELTRANENA E,ZIJLSTRA R T.Diet nutrient digestibility and growth performance of weaned pigs fed solvent-extracted Brassica juncea canola meal[J].Animal Feed Science and Technology,2013,180(1/2/3/4):64-72.

[2] KIARIE E,OWUSU-ASIEDU A,PÉRON A,et al.Efficacy of xylanase and β-glucanase blend in mixed grains and grain co-products-based diets for fattening pigs[J].Livestock Science,2012,148(1/2):129-133.

[3] BERROCOSO J D,SERRANO M P,CÁMARA L,et al.Influence of diet complexity on productive performance and nutrient digestibility of weanling pigs[J].Animal Feed Science and Technology,2012,171(2/3/4):214-222.

[4] KAVANAGH S,LYNCH P B,O'MARA F,et al.A comparison of total collection and marker technique for the measurement of apparent digestibility of diets for growing pigs[J].Animal Feed Science and Technology,2001,89(1/2):49-58.

[5] ADEOLA O,YOUNG L G,MCMILLAN E G,et al.Comparative protein and energy value of OAC Wintri triticale and corn for pigs[J].Journal of Animal Science,1986,63(6):1854-1861.

[6] JAGGER S,WISEMAN J,COLE D J A,et al.Evaluation of inert markers for the determination of ileal and faecal apparent digestibility values in the pig[J].British Journal of Nutrition,1992,68(3):729-739.  

[7] SCOTT T A,HALL J W.Using acid insoluble ash marker ratios (diet:digesta) to predict digestibility of wheat and barley metabolizable energy and nitrogen retention in broiler chicks[J].Poultry Science,1998,77(5):674-679.  

[8] SCOTT T A,BOLDAJI F.Comparison of inert markers [chromic oxide or insoluble ash (Celite)] for determining apparent metabolizable energy of wheat- or barley-based broiler diets with or without enzymes[J].Poultry Science,1997,76(4):594-598.  

[9] GUEVARA P,CLAEYS T,JANSSENS G.Apparent digestibility in meat-type guinea pigs as determined by total collection or by internal marker[J].Veterinarni Medicina,2008,53(4):203-206.

[10] REGMI P R,FERGUSON N S,ZIJLSTRA R T.In vitro digestibility techniques to predict apparent total tract energy digestibility of wheat in grower pigs[J].Journal of Animal Science,2009,87(11):3620-3629.  

[11] DE COCA-SINOVA A,MATEOS G G,GONZÁLEZ-ALVARADO J M,et al.Comparative study of two analytical procedures for the determination of acid insoluble ash for evaluation of nutrient retention in broilers[J].Spanish Journal of Agricultural Research,2011,9(3):761-768.  

[12] HILL R C,BURROWS C F,ELLISON G W,et al.The use of chromic oxide as a marker for measuring small intestinal digestibility in cannulated dogs[J].Journal of Animal Science,1996,74(7):1629-1634.

[13] YIN Y L,MCEVOY J D G,SCHULZE H,et al.Apparent digestibility (ileal and overall) of nutrients as evaluated with PVTC-cannulated or ileo-rectal anastomised pigs fed diets containing two indigestible markers[J].Livestock Production Science,2000,62(2):133-141.  

[14] 陈瑶,邢壮,莫放,等.全收粪和指示剂法测定阉牛日粮有机物消化率的差异性分析[J].畜牧兽医学报,2008,39(2):176-181.

[15] 张晋辉,李德发,朴香淑,等.应用不同指示剂测定羽毛粉的氨基酸回肠末端表观消化率[C]//中国畜牧兽医学会动物营养学分会第六届全国会员代表大会暨第八届学术研讨会论文集(下).北京:中国畜牧兽医学会,2000.

[16] DOURADO L R B,SIQUEIRA J C,SAKOMURA N K,et al.Poultry feed metabolizable energy determination using total or partial excreta collection methods[J].Revista Brasileira de Ciência Avícola,2010,12(2):129-132.  

[17] MOUGHAN P J,SMITH W C,SCHRAMA J,et al.Chromic oxide and acid-insoluble ash as faecal markers in digestibility studies with young growing pigs[J].New Zealand Journal of Agricultural Research,1991,34(1):85-88.  

[18] THONNEY M L,PALHOF B A,DECARLO M R,et al.Sources of variation of dry matter digestibility measured by the acid insoluble ash marker[J].Journal of Dairy Science,1985,68(3):661-668.  

[19] LÆRKE H N,KASPRZAK M M,KNUDSEN K E B.Evaluation of two external markers for measurement of ileal and total tract digestibility of pigs fed human-type diets[J].Journal of Animal Science,2012,90(Suppl.4):384-386.

[20] LAN Y,OPAPEJU F O,NYACHOTI C M.True ileal protein and amino acid digestibilities in wheat dried distillers' grains with solubles fed to finishing pigs[J].Animal Feed Science and Technology,2008,140(1/2):155-163.

[21] VAN KEULEN J,YOUNG B A.Evaluation of acid-insoluble ash as a natural marker in ruminant digestibility studies[J].Journal of Animal Science,1977,44(2):282-287.

[22] MC CARTHY J F,BOWLAND J P,AHERNE F X.Influence of method upon the determination of apparent digestibility in the pig[J].Canadian Journal of Animal Science,1977,57(1):131-135.  

[23] STEIN H H,SHURSON G C.BOARD-INVITED REVIEW:the use and application of distillers dried grains with solubles in swine diets[J].Journal of Animal Science,2009,87(4):1292-1303.  

[24] BAKKER G C M,JONGBLOED A W.The effect of housing system on apparent digestibility in pigs,using the classical and marker (chromic oxide,acid-insoluble ash) techniques,in relation to dietary composition[J].Journal of the Science of Food and Agriculture,1994,64(1):107-115.  

[25] VAN LEEUWEN P,VELDMAN A,BOISEN S,et al.Apparent ileal dry matter and crude protein digestibility of rations fed to pigs and determined with the use of chromic oxide (Cr2O3) and acid-insoluble ash as digestive markers[J].British Journal of Nutrition,1996,76(4):551-562.  

[26] FURUYA S,YAMAMOTO A,ITOH M,et al.Use of acid-insoluble ash added with celite as a marker for determining digestibility in pigs[J].Japanese Journal of Swine Husbandry Research,2001,38(4):171-176.  
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