研究简报 Short Communications

乙酸对奶牛乳腺上皮细胞乳脂肪酸从头合成相关基因表达量的影响

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  • 内蒙古农业大学动物科学学院, 呼和浩特 010018
韩慧娜(1988—),女,内蒙古呼和浩特人,硕士研究生,从事反刍动物营养的研究。E-mail:hanhuina123@sohu.com

收稿日期: 2014-09-22

  网络出版日期: 2015-03-18

基金资助

奶业"973计划"(2011CB100800)

Effects of Acetic Acid on Expression Levels of Genes Involved in Milk Fatty Acids de Novo Synthesis in Bovine Mammary Epithelial Cells

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  • College of Animal Science, Inner Mongolia Agricultural University, Huhhot 010018, China

Received date: 2014-09-22

  Online published: 2015-03-18

摘要

本试验旨在研究不同浓度的乙酸对奶牛乳腺上皮细胞(bovine mammary epithelial cells,BMECs)乳脂肪酸从头合成相关基因表达量的影响。将传至第3代的BMECs悬液接种于细胞培养板上,每孔加入含10%胎牛血清(FBS)的DMEM/F12培养液,于37 ℃ 5% CO2培养箱培养48 h。再将培养48 h的奶牛BMECs培养孔随机分配到6组中,每组6个重复,每个重复1个孔。在各组中分别加入含不同浓度乙酸的DMEM/F12培养液,培养液中的FBS用1 g/L无脂肪酸的牛血清白蛋白(BSA)代替,并使反应体系中乙酸的最终浓度分别为0(对照)、4、6、8、10、12 mmol/L。置于37 ℃ 5% CO2培养箱继续培养48 h。结果表明:BMECs内甘油三酯的含量随着乙酸浓度的增加呈极显著的一元线性增加(P=0.000),以8~12 mmol/L的乙酸组促进作用较好。脂肪酸合成酶(fatty acid synthase,FASN)和乙酰辅酶A羧化酶(acetyl-coenzyme A carboxylase α,ACACA)mRNA的相对表达量随着乙酸添加浓度的增加呈极显著的一次线性增加(P=0.000),以8~12 mmol/L乙酸组的表达量较高。硬脂酰辅酶A去饱和酶(stearoyl-CoA desaturase,SCD)mRNA相对表达量随着乙酸浓度的增加,变化趋势不显著(P>0.05),但从数值上看,所有试验组均高于对照组,以4 mmol/L乙酸组最高。综合得出,乙酸对奶牛BMECs内乳脂肪的合成及其乳脂肪合成相关基因FASNACACA mRNA的表达量有极显著的提高效果,其中以培养液中8~12 mmol/L的乙酸效果较好。

本文引用格式

韩慧娜, 闫素梅, 齐利枝, 生冉, 赵艳丽 . 乙酸对奶牛乳腺上皮细胞乳脂肪酸从头合成相关基因表达量的影响[J]. 动物营养学报, 2015 , 27(3) : 926 -931 . DOI: 10.3969/j.issn.1006-267x.2015.03.033

Abstract

The purpose of this study was to examine the effects of acetic acid on expression levels of genes involved in milk fatty acid de novo synthesis in bovine mammary epithelial cells (BMECs). The 3th passage cells were plated in culture plates with DMEM/F12 medium containing 10% fetal bovine serum (FBS), then the plates were maintained in an incubator with 5% CO2 and at 37 ℃. After 48 h, cells were distributed to six groups and cultured in DMEM/F12 medium containing different concentrations of acetic acid

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