动物营养学报 >

2015 , Vol. 27 >Issue 3: 932 - 940

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.03.034

研究简报 Short Communications

不同代谢葡萄糖水平对绵羊人工瘤胃发酵特性、微生物蛋白质浓度和产气参数的影响

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  • 扬州大学动物科学与技术学院, 扬州 225009
赵睿(1990—),男,山西太原人,硕士研究生,动物营养与饲料科学专业。E-mail:734948896@qq.com

收稿日期: 2014-09-09

  网络出版日期: 2015-03-18

基金资助

国家公益性行业(农业)科研专项"南方地区肉羊育肥与高品质肉生产技术研究"(201303144);江苏高校优势学科建设工程资助项目(PAPD)

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Effects of Different Levels of Metabolic Glucose on Artificial Rumen Fermentation Characteristics, Microbial Protein Concentration and Gas Production Parameters of Sheep

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  • College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received date: 2014-09-09

  Online published: 2015-03-18

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摘要

本试验旨在通过人工瘤胃体外培养,研究不同代谢葡萄糖水平下的绵羊瘤胃发酵特性、微生物蛋白质浓度和产气参数。采用单因子试验设计,共设计4个代谢葡萄糖水平[125(A)、138(B)、153(C)、168 g/kg(D)]。体外试验所用瘤胃液采自4只安装有永久性瘤胃瘘管的绵羊。分别于培养0、2、4、6、8、12、24 h采集2 mL培养液用于分析。结果表明:1)8~24 h培养液pH随着代谢葡萄糖水平的提高而出现显著或极显著下降(P<0.05或P<0.01);氨氮浓度在2 h时D组显著高于A组(P<0.05),而在6 h时A组显著高于其他3组(P<0.05)。2)D组6 h培养液细菌蛋白浓度显著高于A组(P<0.05);随着代谢葡萄糖水平的提高,培养液丙酸、丁酸、总挥发性脂肪酸的浓度总体呈升高趋势,乙酸/丙酸呈下降趋势。3)随着代谢葡萄糖水平的提高,理论最大产气量极显著降低(P<0.01),达1/2理论最大产气量的时间极显著缩短(P<0.01);潜在产气量无显著变化(P>0.05),24 h产气量和产气速率常数分别显著或极显著下降和上升(P<0.05或P<0.01)。结果提示,提高代谢葡萄糖水平,可以提高丙酸、总挥发性脂肪酸的浓度,同时可为绵羊提供较多的生糖前体物质。

关键词： 绵羊; 瘤胃发酵; 代谢葡萄糖; 微生物蛋白质

本文引用格式

赵睿, 徐进昊, 李华伟, 王洪荣 . 不同代谢葡萄糖水平对绵羊人工瘤胃发酵特性、微生物蛋白质浓度和产气参数的影响[J]. 动物营养学报, 2015 , 27(3) : 932 -940 . DOI: 10.3969/j.issn.1006-267x.2015.03.034

Abstract

This experiment aimed to investigate rumen fermentation characteristics, microbial protein concentration and gas production parameters of sheep under the different levels of metabolic glucose through the in vitro artificial rumen fermentation. A single factor experiment design with 4 metabolic glucose levels [125 (A), 138 (B), 153 (C) and 168 g/kg(D)] was adopted. Culture medium of 2 mL was collected for analysis at 0, 2, 4, 6, 8, 12 and 24 hours, respectively. The results showed as follows: 1) with the increasing of metabolic glucose level, the pH of culture medium was significantly decreased at 8 to 24 h (P<0.05 or P<0.01); at 2 h, the ammonia nitrogen concentration of group D was significantly higher than that of group A (P<0.05), while at 6 h, the concentration of group A was significantly higher than that of the other three groups (P<0.05). 2) The bacterial protein concentration at 6 h of group D was significantly higher than that of group A (P<0.05); with the increasing of metabolic glucose level, the concentrations of propionic acid, butyric acid and total volatile fatty acids were increased in general, while acetate/propionate was decreased. 3) With the increasing of metabolic glucose level, the theological maximum gas production and reaching time of 1/2 of the theological maximum gas production were significantly reduced (P<0.01); the potential gas production has no significant change (P>0.05); the 24 h gas production and the constant of gas production rate were significantly decreased and increased (P<0.05 or P<0.01), respectively. The results indicate that the increasing of metabolic glucose level can improve the concentrations of propionic acid and total volatile fatty acids, meanwhile, can provide more sugar precursors for the sheep.

Key words： sheep; rumen fermentation; metabolic glucose; microbial protein

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