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牛磺酸对脂多糖诱导的小鼠肝脏损伤的缓解作用

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  • 江西农业大学, 南昌 330045
付凌(1979—),女,江西进贤人,讲师,硕士,从事动物营养与疾病预防研究。E-mail:jxndfl@sohu.com

收稿日期: 2014-10-21

  网络出版日期: 2015-03-18

基金资助

江西农业大学博士科研启动基金(209004532)

Mitigative Effect of Taurine on Lipopolysaccharides-Induced Liver Injury in Mice

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  • Jiangxi Agricultural University, Nanchang 330045, China

Received date: 2014-10-21

  Online published: 2015-03-18

摘要

本试验旨在研究牛磺酸对脂多糖(LPS)诱导的小鼠肝脏损伤的缓解作用。选用30只体重(22±3) g的ICR雄性小鼠,随机分为3组(每组10只小鼠,n=10):对照组和LPS组饲喂基础饲粮,牛磺酸组在基础饲粮中添加2.5%的牛磺酸。饲喂1周后LPS组和牛磺酸组小鼠腹腔注射10 mg/kg的LPS(LPS溶解于生理盐水,注射剂量为0.2 mL/只),对照组小鼠腹腔注射等体积的生理盐水。LPS注射24 h后,各组小鼠眼眶采血并处死,采集肝脏。血液用于血清谷丙转氨酶(ALT)和谷草转氨酶(AST)活性的测定,肝脏称重后检测氧化应激参数,抗氧化基因及转录因子E2相关因子2(Nrf2)、Kelch样ECH相关蛋白(Keap1)的相对表达量。结果表明:1)LPS处理显著升高了小鼠的肝脏指数(P<0.05),而添加牛磺酸显著抑制了由LPS引起的肝脏指数升高(P<0.05)。2)LPS处理显著增加了血清ALT和AST活性(P<0.05),添加牛磺酸后抑制了血清ALT和AST活性的增加,且其AST活性达到对照组水平(P>0.05)。3)LPS组小鼠肝脏出现明显的损伤,而牛磺酸组小鼠肝脏组织损伤较轻。4)与对照组相比,LPS组小鼠肝脏丙二醛(MDA)含量显著上升(P<0.05),谷胱甘肽过氧化物酶(GPx)活性显著降低(P<0.05),而牛磺酸组上述指标未发生显著变化(P>0.05)。5)牛磺酸显著缓解了LPS对肝脏GPx1和Nrf2表达的抑制作用(P<0.05)。综上所述,饲粮中添加2.5%的牛磺酸对LPS诱导的小鼠肝脏损伤起到了一定的缓解作用。

本文引用格式

付凌, 宋剑波, 胡春燕, 曾黎明 . 牛磺酸对脂多糖诱导的小鼠肝脏损伤的缓解作用[J]. 动物营养学报, 2015 , 27(3) : 989 -996 . DOI: 10.3969/j.issn.1006-267x.2015.03.040

Abstract

This study aimed to investigate the mitigative effect of taurine on lipopolysaccharides (LPS)-induced liver injury in mice. Thirty ICR male mice with the similar body weight of (22±3) g were randomly divided into three groups (ten mice in each group, n=10): a control group and a LPS group in which mice received a basal diet, and a taurine group in which mice received the basal diet+2.5% taurine. After fed 1 week, mice in the LPS group and taurine group were intraperitoneally injected 10 mg/kg LPS (LPS was dissolved in physiological saline, and injection dose was 0.2 mL per mouse) and mice in the control group were intraperitoneally injected the same dose of physiological saline. Blood samples were harvested from eyes and all mice were killed at 24 h after injection to collect liver. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities were measured and liver weight, oxidative stress parameters, and the relative expression of antioxidant genes, nuclear factor E2-related factor 2 (Nrf2) and Kelch like ECH associated protein 1 (Keap1) were determined. The results showed as follows: 1) LPS treatment significantly increased liver index, while taurine supplementation significantly reduced the liver index to normal level (P<0.05). 2) LPS treatment significantly enhanced serum ALT and AST activities (P<0.05), and taurine supplementation exhibited some beneficial effects on serum ALT and AST activities, and no significant difference in serum AST activity between taurine group and control group (P>0.05). 3) LPS caused marked liver injury in mice, and mice in taurine group suffered little liver injury. 4) Compared with control group, liver malondialdehyde (MDA) content was significantly increased and live glutathione peroxidase (GPx) activity was significantly decreased in LPS group (P<0.05), while those parameters in taurine group had no significant changes (P>0.05). 5) Taurine significantly mitigated the LPS-induced inhibition of GPx1 and Nrf2 expression (P<0.05). In conclusion, diet supplemented with 2.5% taurine plays a certain mitigative effect on LPS-induced liver injury in mice.

参考文献

[1] RIPPS H,SHEN W.Review:taurine:a "very essential" amino acid[J].Molecular Vision,2012,18:2673-2686.

[2] STIPANUK M H,UEKI I.Dealing with methionine/homocysteine sulfur:cysteine metabolism to taurine and inorganic sulfur[J].Journal of Inherited Metabolic Disease,2011,34(1):17-32.  

[3] STIPANUK M H.Role of the liver in regulation of body cysteine and taurine levels:a brief review[J].Neurochemical Research,2004,29(1):105-110.  

[4] WEI S D,HUANG Q Y,LI J Z,et al.Taurine attenuates liver injury by downregulating phosphorylated p38 MAPK of Kupffer cells in rats with severe acute pancreatitis[J].Inflammation,2012,35(2):690-701.  

[5] REDMOND H P,WANG J H,BOUCHIER-HAYES D.Taurine attenuates nitric oxide-and reactive oxygen intermediate-dependent hepatocyte injury[J].Archives of Surgery,1996,131(12):1280-1287.  

[6] MAIA A R,BATISTA T M,VICTORIO J A,et al.Taurine supplementation reduces blood pressure and prevents endothelial dysfunction and oxidative stress in post-weaning protein-restricted rats[J].PLoS One,2014,9(8):e105851.

[7] LI Q,LIU Y L,CHE Z Q,et al.Dietary L-arginine supplementation alleviates liver injury caused by Escherichia coli LPS in weaned pigs[J].Innate Immunity,2012,18(6):804-814.  

[8] ROLLER J,LASCHKE M W,SCHEUER C,et al.Heme oxygenase (HO)-1 protects from lipopolysaccharide (LPS)-mediated liver injury by inhibition of hepatic leukocyte accumulation and improvement of microvascular perfusion[J].Langenbeck's Archives of Surgery,2010,395(4):387-394.  

[9] GURUNG R B,PURBE B,GYAWALI P,et al.The ratio of aspartate aminotransferase to alanine aminotransferase (AST/ALT):the correlation of value with underlying severity of alcoholic liver disease[J].Kathmandu University Medical Journal,2013,11(43):233-236.

[10] TASCI I,MAS N,MAS M R,et al.Ultrastructural changes in hepatocytes after taurine treatment in CCl4 induced liver injury[J].World Journal of Gastroenterology,2008,14(31):4897-4902.  

[11] WETTSTEIN M,HÄUSSINGER D.Taurine attenuates cold ischemia-reoxygenation injury in rat liver[J].Transplantation,2000,69(11):2290-2296.  

[12] DEMINICE R,ROSA F T,DA SILVA L E C M,et al.Taurine supplementation does not decrease homocysteine levels and liver injury induced by a choline-deficient diet[J].Life Sciences,2014,105(1/2):43-47.

[13] LIU Y T,YANG L Q,TAO K M,et al.Protective effects of hydrogen enriched saline on liver ischemia reperfusion injury by reducing oxidative stress and HMGB1 release[J].BMC Gastroenterology,2014,14(1):12.

[14] MATSUO K,SASAKI E,HIGUCHI S,et al.Involvement of oxidative stress and immune- and inflammation-related factors in azathioprine-induced liver injury[J].Toxicology Letters,2014,224(2):215-224.  

[15] ZHU X,ZHANG F,ZHOU L,et al.Diallyl trisulfide attenuates carbon tetrachloride-caused liver injury and fibrogenesis and reduces hepatic oxidative stress in rats[J].Naunyn-Schmiedeberg's Archives of Pharmacology,2014,387(5):445-455.  

[16] KIM K J,HONG H D,LEE O H,et al.The effects of Acanthopanax senticosus on global hepatic gene expression in rats subjected to heat environmental stress[J].Toxicology,2010,278(2):217-223.  

[17] ZHANG Z Y, LIU D,YI B,et al.Taurine supplementation reduces oxidative stress and protects the liver in an iron-overload murine model[J].Molecular Medicine Reports,2014,10(5):2255-2262.

[18] JEON S H,LEE M Y,RAHMAN M M,et al.The antioxidant,taurine reduced lipopolysaccharide (LPS)-induced generation of ROS,and activation of MAPKs and Bax in cultured pneumocytes[J].Pulmonary Pharmacology & Therapeutics,2009,22(6):562-566.  

[19] CHANG Y Y,CHOU C H,CHIU C H,et al.Preventive effects of taurine on development of hepatic steatosis induced by a high-fat/cholesterol dietary habit[J].Journal of Agricultural and Food Chemistry,2011,59(1):450-457.  

[20] CHEN X,SEBASTIAN B M,TANG H,et al.Taurine supplementation prevents ethanol-induced decrease in serum adiponectin and reduces hepatic steatosis in rats[J].Hepatology,2009,49(5):1554-1562.  

[21] KURZATKOWSKI D M,TROMBETTA L D.Maneb causes pro-oxidant effects in the hippocampus of Nrf2 knockout mice[J].Environmental Toxicology and Pharmacology,2013,36(2):427-436.  

[22] WRUCK C J,FRAGOULIS A,GURZYNSKI A,et al.Role of oxidative stress in rheumatoid arthritis:insights from the Nrf2-knockout mice[J].Annals of the Rheumatic Diseases,2011,70(5):844-850.  

[23] GIUDICE A,ARRA C,TURCO M C.Review of molecular mechanisms involved in the activation of the Nrf2-ARE signaling pathway by chemopreventive agents[J].Methods in Molecular Biology,2010,647:37-74.

[24] SINHA D,BISWAS J,BISHAYEE A.Nrf2-mediated redox signaling in arsenic carcinogenesis:a review[J].Archives of Toxicology,2013,87(2):383-396.  

[25] STEPKOWSKI T M,KRUSZEWSKI M K.Molecular cross-talk between the NRF2/KEAP1 signaling pathway,autophagy,and apoptosis[J].Free Radical Biology and Medicine,2011,50(9):1186-1195.  

[26] AGCA C A,TUZCU M,HAYIRLI A,et al.Taurine ameliorates neuropathy via regulating NF-κB and Nrf2/HO-1 signaling cascades in diabetic rats[J].Food and Chemical Toxicology,2014,71:116-121.

[27] JANG J S,PIAO S Y,CHA Y N,et al.Taurine chloramine activates Nrf2,increases HO-1 expression and protects cells from death caused by hydrogen peroxide[J].Journal of Clinical Biochemistry and Nutrition,2009,45(1):37-43.  
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