动物营养学报 >

2015 , Vol. 27 >Issue 5: 1440 - 1449

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.05.014

特种经济动物营养 Special Economic Animal Nutrition

意大利蜜蜂工蜂幼虫饲粮中亚油酸的适宜添加水平

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  • 山东农业大学动物科技学院, 泰安 271018
王圣伟(1990-),男,河南商丘人,硕士研究生,从事蜜蜂营养研究。E-mail:swwang2012@163.com

收稿日期: 2014-12-19

  网络出版日期: 2015-05-13

基金资助

国家蜂产业技术体系建设专项资金(CARS-45);山东省农业良种工程项目"优质高产蜜蜂及蚕桑新品种培育"(2014-2016)

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Appropriate Linoleic Acid Supplemental Level in Apis mellifera ligustica Worker Larvae Diet

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  • College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271018, China

Received date: 2014-12-19

  Online published: 2015-05-13

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摘要

本试验旨在研究意大利蜜蜂工蜂幼虫饲粮中亚油酸的适宜添加水平。选取576只1日龄的工蜂幼虫,随机分为6组,每组96只幼虫,对照饲喂基础饲粮,试验组分别饲喂添加0.02%、0.04%、0.06%、0.08%和0.10%亚油酸的饲粮,试验期7 d。结果表明:1)与对照组相比,饲粮添加0.10%亚油酸显著降低了化蛹率(P<0.05)。2)与对照组相比,饲粮添加0.04%亚油酸显著提高了5日龄虫体蛋白质浓度(P<0.05);饲粮中添加0.10%亚油酸显著降低了7日龄蛹蛋白质浓度(P<0.05)。3)与对照组相比,饲粮添加0.02%亚油酸对6日龄虫体的过氧化物酶增殖物激活受体(PPAR)α活性有显著的抑制作用(P<0.05),添加0.08%的亚油酸则显著提高了6日龄虫体PPARα和PPARγ活性。4)与对照组相比,饲粮添加0.04%和0.08%亚油酸显著提高了6日龄虫体脂肪酸合成酶(FAS)活性(P<0.05);添加0.08%亚油酸显著提高了5日龄虫体乙酰辅酶A羧化酶(ACC)浓度(P<0.05),添加0.06%和0.10%亚油酸显著提高了6日龄虫体ACC浓度(P<0.05)。5)饲料中添加本试验设定范围的亚油酸对幼虫FAS和ACC基因相对表达量无显著影响(P>0.05)。综合化蛹率和虫体蛋白质浓度2个指标的拟合曲线结果,认为意大利蜜蜂工蜂幼虫饲粮中亚油酸的适宜添加水平为0.024%~0.037%。

关键词： 亚油酸; 意大利蜜蜂; 适宜添加水平; 化蛹率; 蛋白浓度; 脂肪代谢

本文引用格式

王圣伟, 胥保华, 王红芳 . 意大利蜜蜂工蜂幼虫饲粮中亚油酸的适宜添加水平[J]. 动物营养学报, 2015 , 27(5) : 1440 -1449 . DOI: 10.3969/j.issn.1006-267x.2015.05.014

Abstract

This experiment was conducted to study the appropriate linoleic acid supplemental level in Apis mellifera ligustica worker larvae diet. Five hundred and seventy six larvae were randomly assigned to 6 groups with 96 larvae in each group. Larvae in control group were fed a basal diet, and those in experimental groups were fed the basal diet supplemented with 0.02%, 0.04%, 0.06%, 0.08% and 0.10% linoleic acid, respectively. The experiment lasted for 7 days. The results showed as follows: 1) compared with the control group, the diet supplemented with 0.10% linoleic acid reduced the percentage of pupation (P<0.05). 2) Compared with the control group, the diet supplemented with 0.04% linoleic acid significantly increased the total protein concentration of larvae at 5 days old (P<0.05), when the worker larvae developed into pupa at 7 days old, the diet supplemented with 0.10% linoleic acid significantly increased the pupae protein concentration (P<0.05). 3) Compared with the control group, the diet supplemented with 0.02% linoleic acid had significant suppression effects on the activity of peroxisome proliferators-activated receptor (PPAR)α (P<0.05), but 0.08% linoleic acid had stimulating effects on the activities of PPARα and PPARγ at 6 days old. 4) Compared with the control group, the linoleic acid supplemental levels at 0.04% and 0.08% significantly improved the activity of fatty acid synthetase (FAS) of larvae at 6 days old (P<0.05). Acetyl CoA carboxylase (ACC) concentration of larvae at 5 days old was significantly improved by 0.08% linoleic acid (P<0.05) and ACC concentration of larvae at 6 days old was significantly improved by 0.06% and 0.10% linoleic acid (P<0.05). 5) Different supplemental levels of linoleic acid had no effects on the relative expression levels of FAS and ACC genes (P>0.05). According to the curve fitting results of the percentage of pupation and protein concentration of larvae, it is concluded that the appropriate linoleic supplemental level in Apis mellifera ligustica worker larvae diet is 0.024% to 0.037%.

Key words： linoleic acid; Apis mellifera ligustica; appropriate supplemental level; percentage of pupation; concentration of protein; fat metabolism

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