本试验研究了黄芪多糖(APS)对肉仔鸡十二指肠黏膜的免疫调节作用。200只1日龄爱拔益加(AA)肉鸡随机分成4组,每组50只,Ⅰ组为对照组,Ⅱ、Ⅲ、Ⅳ组为试验组。Ⅱ、Ⅲ、Ⅳ组于5~7日龄分别经口注入3种不同浓度(6、4、2 mg/mL)APS,0.5 mL/只,1次/d,连用3 d,对照组灌服等量生理盐水。7日龄,每只采用鸡新支二联苗2倍量点眼滴鼻免疫。分别于8、15、22日龄取样,测定各组10只肉仔鸡十二指肠黏膜免疫球蛋白含量、白细胞介素-2(IL-2)含量和黏膜免疫相关细胞数量。结果显示:高、中剂量APS可显著提高15日龄肉仔鸡十二指肠黏膜免疫球蛋白A、免疫球蛋白G、免疫球蛋白M含量(P<0.05),高、中剂量APS可显著或极显著提高15、22日龄肉仔鸡十二指肠黏膜IL-2含量和上皮内淋巴细胞、肥大细胞、杯状细胞数量(P<0.05或P<0.01)。由此可见,APS可增强肉仔鸡十二指肠黏膜免疫功能,以6、4 mg/mL剂量最佳。

This experiment was conducted to investigate the modulator effects of astragals polysaccharides (APS) on duodenum mucosal immunity of broilers. Two hundred Arbor Acres broilers were randomly divided into four groups of 50 broilers per group: group Ⅰ was control group, while the other 3 groups were experimental groups which broilers were orally administered with 0.5 mL APS at 3 different concentrations (6, 4, 2 mg/mL) once per day for three days, and broilers in group Ⅰ were oral administratied the same volume of saline. All broilers at 7 days of age were vaccined with combined vaccine against Newcastle disease and infectious bronchitis with 2 times the volume by drop and nasal immunization. Duodenum mucosa was sampled on 8, 15, 22 days of age with ten broilers for detecting contents of immunoglobulin and interleukin-2 (IL-2), and for measurement of the numbers of mucosal immunity-associated cells, respectively. The results showed as follows: contents of immunoglobulin A, immunoglobulin G, immunoglobulin M of duodenum mucosa were significantly higher in broilers at age of 15 days drinking 6 and 4 mg/mL APS than the control (P<0.05). Content of IL-2 in duodenum mucosa and the numbers of intraepithelial lymphocyte, goblet cell and mast cell were significantly higher in broilers at age of 15 and 22 days drinking 6 and 4 mg/mL APS than the control (P<0.05 or P<0.01). It is concluded that oral administration of APS enhances duodenum mucosal immunity of broilers, and the proper concentration of APS was 6 and 4 mg/mL.