动物营养学报 >

2015 , Vol. 27 >Issue 5: 1596 - 1604

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.05.032

研究简报 Short Communications

饲料维生素E水平影响云纹石斑鱼幼鱼对氨氮胁迫的响应

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  • 1. 中国水产科学研究院东海水产研究所, 农业部东海与远洋渔业资源开发利用重点实验室, 上海 200090;
    2. 上海海洋大学水产与生命学院, 上海 201306
施兆鸿(1958-),男,上海人,研究员,主要从事海水鱼类繁育生物学研究。E-mail:shizh@eastfishery.ac.cn

收稿日期: 2014-12-23

  网络出版日期: 2015-05-13

基金资助

国家科技支撑项目(2011BAD13B01)

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Dietary Vitamin E Level Affects the Response of Juvenile Epinehelus moara to Ammonia Nitrogen Stress

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  • 1. Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation, Ministry of Agriculture, East Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China;
    2. College of Fisheries and Life, Shanghai Ocean University, Shanghai 201306, China

Received date: 2014-12-23

  Online published: 2015-05-13

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摘要

为观察饲料中维生素E水平在云纹石斑鱼(Epinehelus moara)幼鱼抗氨氮胁迫中的作用,试验配制了维生素E水平分别为11.09、47.52、91.38、134.57、178.92 mg/kg的试验饲料,饲喂初始体重为(15.6±0.2) g的云纹石斑鱼幼鱼56 d后进行6 h氨氮胁迫,测定其血清葡萄糖(GLU)、乳酸(LD)、皮质醇(COR)、丙二醛(MDA)含量,超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)活力,总抗氧化能力(T-AOC)以及肝脏SOD活力和MDA含量对氨氮胁迫的响应。每个维生素E水平设3个网箱(重复),每个网箱放养20尾鱼。结果显示:各组血清中GLU含量在饲喂前后无显著差异(P>0.05),氨氮胁迫后血清中GLU含量随饲料中维生素E水平的升高而降低。氨氮胁迫后血清中LD含量与饲料中维生素E水平呈反比,并在饲料中维生素E水平达到47.52 mg/kg之后维持稳定。氨氮胁迫后各组血清中COR含量都有显著升高(P<0.05)。当饲料中维生素E水平达到47.52 mg/kg后,饲喂56 d后再氨氮胁迫对血清和肝脏中SOD活力不产生显著影响(P>0.05)。血清中CAT活力在饲喂后和氨氮胁迫后均有下降的趋势,当饲料中维生素E水平达到或超过91.38 mg/kg时不论饲喂后还是氨氮胁迫后,血清中CAT活力变化都不显著(P>0.05)。在低维生素E水平(11.09和47.52 mg/kg)下,血清和肝脏中MDA含量在氨氮胁迫后均呈显著上升(P<0.05);在高维生素E水平(91.38、134.57、178.92 mg/kg)下,血清和肝脏中MDA含量在氨氮胁迫后没有产生显著差异(P>0.05)。在饲料维生素E水平达到或超过91.38 mg/kg时,饲喂后血清中GSH-Px活力均显著上升(P<0.05),氨氮胁迫后血清中GSH-Px活力则无显著变化(P>0.05)。在饲料维生素E水平达到或超过47.52 mg/kg时,血清T-AOC在饲喂后呈现显著升高(P<0.05),而氨氮胁迫后又出现下降的趋势。由此得出,在本试验条件下,饲料中维生素E水平与云纹石斑鱼幼鱼的抗应激和抗氧化能力具有相关性,饲料中维生素E水平达到或超过91.38 mg/kg可有效地提高云纹石斑鱼幼鱼的抗应激和抗氧化能力。

关键词： 维生素E; 云纹石斑鱼; 氨氮胁迫; 抗应激能力; 抗氧化能力

本文引用格式

施兆鸿, 张艳亮, 高权新, 彭士明, 张晨捷 . 饲料维生素E水平影响云纹石斑鱼幼鱼对氨氮胁迫的响应[J]. 动物营养学报, 2015 , 27(5) : 1596 -1604 . DOI: 10.3969/j.issn.1006-267x.2015.05.032

Abstract

This study was conducted to determine the effect of vitamin E level on juvenile Epinephelus moara under ammonia nitrogen stress. Five diets were formulated to contain 11.09, 47.52, 91.38, 134.57 and 178.92 mg/kg of vitamin E, respectively. Juvenile Epinephelus moara with the initial body weight of (15.6±0.2) g were selected as the experimental animals. After 56 days of feeding, all fish were exposed to ammonia nitrogen for 6 h, and then the responses of serum glucose (GLU), lactate (LD), cortisol (COR) and malondialdehyde (MDA) contents, serum superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) activities and total antioxidant capacity (T-AOC), and liver SOD activity and MDA content to ammonia nitrogen stress were measured. Each vitamin E level had 3 net cages (replicates) and each net cage cultured 20 fish. The results showed as follows: after feeding, the serum GLU content of all groups showed no significantly difference compared with before feeding (P>0.05) and the serum GLU content was decreased with the dietary vitamin E level increasing after ammonia nitrogen stress. There was an inverse relationship between the serum LD content and dietary vitamin E level after ammonia nitrogen stress, and the serum LD content showed stable when dietary vitamin E level arrived at 47.52 mg/kg. After ammonia nitrogen stress, serum COR content of all groups was significantly increased (P<0.05). When the vitamin E level was equal or higher than 47.52 mg/kg, the serum and liver SOD activity was not significantly affected by the ammonia nitrogen stress after 56 days of feeding (P>0.05). The serum CAT activity was decreased after feeding and ammonia nitrogen stress, while this difference was not significant when the dietary vitamin E level was equal or higher than 91.38 mg/kg neither after feeding or ammonia nitrogen stress (P>0.05). The serum and liver MDA content showed a significant rising trend when fish fed diets containing low levels (11.09 and 47.52 mg/kg) of vitamin E after ammonia nitrogen stress (P<0.05), and no significant difference was observed when fish fed high levels (91.38, 134.57 and 178.92 mg/kg) of vitamin E (P>0.05). When the dietary vitamin E level was equal or higher than 91.38 mg/kg, the serum GSH-Px activity decreased significantly after feeding (P<0.05), but no significant change was found after ammonia nitrogen stress (P>0.05). When the dietary vitamin E level was equal or higher than 47.52 mg/kg, the serum T-AOC increased significantly after feeding (P<0.05), and then decreased after ammonia nitrogen stress. These results indicate that there is a correlation between the dietary vitamin E levels and the anti-stress and antioxidant capacities under this experimental condition, and the anti-stress and antioxidant capacities can be enhanced when the dietary vitamin E level is equal or higher than 91.38 mg/kg.

Key words： vitamin E; Epinephelus moara; ammonia nitrogen stress; anti-stress capacity; antioxidant capacity

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