动物营养学报 >

2015 , Vol. 27 >Issue 5: 1620 - 1631

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.05.035

研究简报 Short Communications

乙酸酐衍生化气相色谱法测定饲料非淀粉多糖含量时适宜称样量确定依据的研究

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  • 1. 中国农业科学院北京畜牧兽医研究所, 动物营养学国家重点实验室, 北京 100193;
    2. 南京农业大学动物科技学院, 南京 210095
黄庆华(1991—),女,山东菏泽人,硕士研究生,从事饲料养分生物学效价评定的研究。E-mail:hqinghua8686@163.com

收稿日期: 2014-11-30

  网络出版日期: 2015-05-13

基金资助

中国农业科学院科技创新工程(ASTIP-IAS07);国家科技支撑项目课题(2012BAD39B01)

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The Research of Appropriate Sample Weight of the Method for Determining Non-Starch Polysaccharides of Feedstuffs Using Gas-Liquid Chromatography with Aiditol Acetates Derivatives

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  • 1. State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing 100193, China;
    2. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China

Received date: 2014-11-30

  Online published: 2015-05-13

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摘要

本试验旨在探讨乙酸酐衍生化气相色谱法测定饲料非淀粉多糖(NSP)含量时的适宜称样量和变异程度,为确定该方法的测试精度与变异因素提供参考。试验1采用单因素完全随机设计,选取0.10、0.15、0.20、0.25、0.30、0.40和0.50 g 7个参比饲粮纤维的称样水平量,每个水平设3个重复,分别测定可溶和不可溶NSP含量。试验2在适宜称样量范围内,利用参比饲粮纤维分析方法的变异系数,设2个批次,每个批次3个重复。试验3在适宜称样量范围内,测定玉米、豆粕、小麦、小麦麸、棉籽粕、菜籽粕、甜菜籽粕、玉米干酒糟及其可溶物(DDGS)和燕麦麸饲料原料中NSP含量。结果显示:1)0.10、0.15、0.20和0.25 g水平所测得总NSP含量(21.33%~21.88%)无显著差异(P>0.05),但显著高于0.30、0.40和0.50 g水平(18.06%~19.32%,P<0.05)。随称样量的增加(0.10~0.25 g)不溶NSP含量逐渐增加,但差异较小,到0.25 g水平时达到最大值(20.14%),然后线性降低(P<0.05)(0.30~0.50 g)。2)2个批次各单糖、糖醛酸和总NSP含量均无显著差异(P>0.05)。可溶性NSP批内、批间和总变异系数均不超过11.57%,不可溶性NSP批内、批间和总变异系数均低于3.19%,总NSP批内、批间和总变异系数均在3.01%以内。3)饲料原料中总NSP含量为8.59%~65.59%,不可溶性NSP含量均高于可溶性NSP含量,组成NSP的主要单糖为阿拉伯糖、木糖和葡萄糖,谷物副产品中总NSP含量高于完整谷物。结果表明,本试验条件下乙酸酐衍生化气相色谱法所称取样品中NSP含量应不超过55 mg(0.25 g参比饲粮纤维中NSP含量),测定的总NSP含量变异性可控制在3.01%以内。

关键词： 饲料原料; 乙酸酐衍生化气相色谱法; 非淀粉多糖; 称样量; 变异

本文引用格式

黄庆华, 陈亮, 高理想, 刘强, 卢凌, 唐湘方, 刘蕾, 张宏福 . 乙酸酐衍生化气相色谱法测定饲料非淀粉多糖含量时适宜称样量确定依据的研究[J]. 动物营养学报, 2015 , 27(5) : 1620 -1631 . DOI: 10.3969/j.issn.1006-267x.2015.05.035

Abstract

The objectives of the present study was investigated appropriate sample weight and variation of the method for determining non-starch polysaccharides (NSP) of feedstuffs by gas chromatography with aiditol acetates derivatives to confirm the precision of assay and the variation of determined values. A single factorial completely randomized design with 7 sample weight treatments (0.10, 0.15, 0.20, 0.25, 0.30, 0.40 and 0.50 g) for reference dietary fiber (RDF) was adapted in Exp. 1 and each treatment contained 3 replicates. The contents of soluble and insoluble NSP of RDF was determined. In Exp. 2, the variation coefficients for determining NSP of RDF based on appropriate sample weight was analyzed in 2 batches with 3 replicates per batch. In Exp.3, the NSP of corn, soybean meal, wheat, wheat bran, cotton meal, rapeseed meal, sugar beet pulp was repeatedly determined based on appropriate sample weight. The results showed as follows: 1) total NSP of RDF with sample weight for 0.10, 0.15, 0.20 and 0.25 g (ranged from 21.33% to 21.88%, P>0.05) were significantly greater than for 0.30, 0.40 and 0.50 g (ranged from 18.06% to 19.32%, P<0.05). The insoluble NSP of RDF increased as the level of sample weight from 0.10 to 0.25 g, whereas the insoluble NSP of RDF significantly decreased by increasing the level of sample weight from 0.25 g to 0.50 g (linear, P<0.05). 2) The contents of monosaccharide, uronic acid, and total NSP were not significantly affected by the batch (P>0.05). The intra-batch, inter-batch and total coefficient of variation (CV) for soluble, insoluble and total NSP were less than 11.57%, 3.19% and 3.01%, respectively. 3) The content of total NSP for selective feed ingredients ranged from 8.59% to 65.59% and soluble NSP was less than the content of insoluble NSP. The primary monosaccharide components of feed ingredients were arabinose, xylose and glucose. The total NSP for cereal was greater than force real by-products. In conclusion, no more than approximate 55 mg NSP (the approximate content of NSP in 0.25 g RDF) of sample weight was used and the CV for total NSP are less than 3.01% in the present method using gas chromatography with aiditol acetates derivatives.

Key words： feedstuff; gas chromatography with aiditol acetates derivatives; non-starch polysaccharides; sample weight; coefficient of variation

参考文献

[1] BACH KNUDSEN K E.Carbohydrate and lignin contents of plant materials used in animal feeding[J].Animal Feed Science Technology,1997,67(4):319-338.  

[2] BACH KNUDSEN K E,HEDEMANN M S,LRKE H N.The role of carbohydrates in intestinal health of pigs[J].Animal Feed Science and Technology,2012,173(1/2):41-53.  

[3] ENGLYST H N,QUIGLEY M E,HUDSON G J,et al.Determination of dietary fiber as non-starch polysaccharides by gas-liquid chromatography[J].Analyst,1992,117(11):1707-1714.  

[4] NRC.Nutrient requirements of swine[M].Washington,D.C.:The National Academies Press,2012.

[5] 贺永惠,王清华,刘长忠,等.乙酸酐衍生化气相色谱法测定小麦非淀粉多糖的单糖组成[J].光谱实验室,2009,26(4):1039-1042.

[6] 许辉,田福利,潘国卿.糖醇乙酯法测定谷物中非淀粉多糖的含量[J].食品工业科技,2004,25(12):125-126.

[7] 龚敏,李铁军,王洁,等.气相色谱法测定麦类农作物中非淀粉多糖含量[J].食品科技,2011,34(5):253-256.

[8] THEANDER O,AMAN P,WESTERLUND E,et al.Total dietary fiber determined as neutral sugar residues,uronic acid residues,and Klason lignin (the Uppsala method):collaborative study[J].Journal of AOAC International,1995,78(4):1030-1044.

[9] ENGLYST H N,QUIGLEY M E,HUDSON G J.Determination of dietary fiber as non-starch polysaccharides with gas-liquid chromatographic,high-performance liquid chromatographic or spectrophotometric measurement of constituent sugars[J].Analyst,1994,119(7):1497-1509.  

[10] LEE S C,PROSKY L,DE VRIES J W.Determination of total,soluble,and insoluble dietary fiber in foods:enzymatic-gravimetric method,MES-TRIS buffer:collaborative study[J].Journal of AOAC International,1992,12:157-165.

[11] CHEN L,ZHANG H F,GAO L X,et al.Effect of graded levels of fiber from alfalfa meal on intestinal nutrient flow and hindgut fermentation of growing pigs[J].Journal of Animal Science,2013,91(10):4757-4764.  

[12] 张亚伟.四种纤维原料组分在猪消化道区段变化规律及相关性的研究[D].硕士学位论文.南京:南京农业大学,2014.

[13] 马云翔,田福利.豆类中非淀粉多糖组分糖醛酸的分光光度法测定[J].分析测试学报,2004,23(1):100-102.

[14] 蒋红卫,夏结来.基于样本变异系数的组间与组内变异统计量[C]//中国卫生统计学术交流大会论文集.武汉:中国卫生信息学会,2006:1-7.

[15] ENGLYST H N,CUMMINGS J H.Simplified method for the measurement of total non-starch polysaccharides by gas-liquid chromatography of constituent sugars as alditol acetates[J].Analyst,1984,109(7):937-942.  

[16] THEANDER O,WESTERLUND E,ÅMAN P,et al.Plant cell walls and monogastric diets[J].Animal Feed Science and Technology,1989,23(1/2/3):205-225.  

[17] FINCHER G B,STONE B A.Cell walls and their components in cereal grain technology[J].Advances in Cereal Science and Technology,1986(8):143-152.
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