鹅源草酸青霉产果胶酶对肥胖模型大鼠体重、脂肪沉积及脂质代谢的影响

徐晓波, 王宝维, 葛文华, 张名爱, 解超

doi:10.3969/j.issn.1006-267x.2015.05.038

动物营养学报 >

2015 , Vol. 27 >Issue 5: 1650 - 1658

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.05.038

研究简报 Short Communications

鹅源草酸青霉产果胶酶对肥胖模型大鼠体重、脂肪沉积及脂质代谢的影响

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青岛农业大学优质水禽研究所, 国家水禽产业技术体系营养与饲料功能研究室, 青岛 266109

徐晓波(1989—),男,山东潍坊人,硕士研究生,研究方向为营养与保健。E-mail:xiaobo45697@126.com

收稿日期: 2014-11-01

网络出版日期: 2015-05-13

基金资助

国家农业科技成果转化资金项目(2013GB2C620543);山东省农业科技成果转化资金项目(鲁科农字2012-65)

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Effects of Pectinase Produced by Penicillium oxalicum Currie & Thom on Body Weight, Lipid Deposition and Lipid Metabolism of Obese Model Rats

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Nutrition and Feed Laboratory of China Agriculture Research System, Institute of High Quality Waterfowl, Qingdao Agricultural University, Qingdao 266109, China

Received date: 2014-11-01

Online published: 2015-05-13

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摘要

本试验旨在研究鹅源草酸青霉产果胶酶对肥胖大鼠体重、脂肪沉积及脂质代谢影响。随机选取雄性大鼠108只,体重220~240 g,适应性饲养3 d。分为对照组(Ⅰ组)、高脂组(Ⅱ组)和高脂模型组;高脂模型组造模成功后,将其按体重随机分为7组,每组3个重复,每个重复4只;各组在基础饲粮中分别添加0(Ⅲ组)、0.01%(Ⅳ组)、0.05%(Ⅴ组)、0.1%(Ⅵ组)、0.2%(Ⅶ组)、0.4%(Ⅷ组)、0.8%(Ⅸ组)的果胶酶。试验期8周。结果表明:1)鹅源草酸青霉产果胶酶能够有效抑制肥胖模型大鼠体重的增长,降低Lee's指数,控制体型发育。2)鹅源草酸青霉产果胶酶能够降低肥胖模型大鼠机体脂肪沉积量。3)鹅源草酸青霉产果胶酶能够有效地对肥胖模型大鼠由于摄入高脂饲粮引起的脂肪肝进行干预,使其恢复到正常水平。4)鹅源草酸青霉产果胶酶能够降低肥胖模型大鼠血清总胆固醇、甘油三酯、低密度脂蛋白胆固醇、游离脂肪酸含量,提高血清高密度脂蛋白胆固醇含量。5)鹅源草酸青霉产果胶酶能够降低肥胖模型大鼠谷丙转氨酶和谷草转氨酶活性,提高血清脂蛋白酯酶和肝酯酶活性。由此可见,鹅源草酸青霉产果胶酶对肥胖模型大鼠脂肪沉积和脂质代谢具有显著干预修复作用,降脂效果明显。

关键词： 鹅源草酸青霉果胶酶; 肥胖大鼠; 脂肪沉积; 脂质代谢

本文引用格式

徐晓波, 王宝维, 葛文华, 张名爱, 解超 . 鹅源草酸青霉产果胶酶对肥胖模型大鼠体重、脂肪沉积及脂质代谢的影响[J]. 动物营养学报, 2015 , 27(5) : 1650 -1658 . DOI: 10.3969/j.issn.1006-267x.2015.05.038

Abstract

This experiment was conducted to study the effects of pectinase produced by Penicillium oxalicum Currie & Thom on body weight, lipid deposition and lipid metabolism of obese model rats. A total of 108 male rats with body weight of 220 to 240 g, adapted feeding 3 days. The rats were divided into control group (group Ⅰ), hyperlipidemia group (group Ⅱ) and other hyperlipidemia model groups. After successful modeling, the rats were randomly divided into 7 groups with 3 replicates per group and 4 rats per replicate. Rats in the 7 groups were fed the basal diets supplemented with 0 (group Ⅲ), 0.01% (group Ⅳ), 0.05% (group Ⅴ), 0.1% (group Ⅵ), 0.2% (group Ⅶ), 0.4% (group Ⅷ) and 0.8% (group Ⅸ) pectinase, respectively. The experiment last for 8 weeks. The results showed as follows: 1) the pectinase produced by Penicillium oxalicum Currie & Thom could restrain the increasing weight, decreased the Lee's index and control portion size of obese model rats. 2) The pectinase could significantly reduce the amount of lipid deposition of obese model rats. 3) The pectinase could restore the fatty liver disease of obese model rats to the normal level due to intake of high-fat diet. 4) The pectinase could reduce the content of total cholesterol, total triglyceride, low-density lipoprotein cholesterol and nonesterified fatty acid in serum of obese model rats, and increase the serum high-density lipoprotein cholesterol content. 5) It also could reduce the activity of aspartate transaminase and alanine aminotransferase in serum of obese model rats, and increase the activity of serum lipoprotein lipase and hepaticendothelaillipase in serum. In conclusion, the pectinase produced by Penicillium oxalicum Currie & Thom has significant effect on reducing the deposition and serum lipid of obese model rats.

Key words： pectinase produced by Penicillium oxalicum Currie &; Thom; obese rats; lipid deposition; lipid metabolism

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