乳铁蛋白对滇撒配套系断奶仔猪生产性能、血清抗氧化指标及组织抗氧化基因表达的影响

安清聪; 李岑曦; 张春勇; 潘洪彬; 李美荃; 徐娜娜; 陈克嶙; 郭荣富

doi:10.3969/j.issn.1006-267x.2015.07.006

动物营养学报 >

2015 , Vol. 27 >Issue 7: 2018 - 2026

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.07.006

猪营养 Swine Nutrition

乳铁蛋白对滇撒配套系断奶仔猪生产性能、血清抗氧化指标及组织抗氧化基因表达的影响

安清聪 ,
李岑曦 ,
张春勇 ,
潘洪彬 ,
李美荃 ,
徐娜娜 ,
陈克嶙 ,
郭荣富

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云南农业大学, 云南省动物营养与饲料重点实验室, 昆明 650201

收稿日期: 2015-02-05

网络出版日期: 2015-07-07

基金资助

云南省重大科技专项基金项目(201212ZA018);云南省现代农业生猪产业技术体系建设(云财教【2013】160#)

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Effects of Lactoferrin on Growth Performance, Serum Antioxidant Indices and Tissue Antioxidant Gene Expressions of Diansa Weaned Piglets

AN Qingcong ,
LI Cenxi ,
ZHANG Chunyong ,
PAN Hongbin ,
LI Meiquan ,
XU Nana ,
CHEN Kelin ,
GUO Rongfu

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Yunnan Key Laboratory of Animal Nutrition and Feeds, Yunnan Agricultural University, Kunming 650201, China

Received date: 2015-02-05

Online published: 2015-07-07

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摘要

本试验旨在研究乳铁蛋白(lactoferrin,LF)对滇撒配套系断奶仔猪生产性能、血清抗氧化指标及组织抗氧化基因表达量的影响。选用(28±2)日龄滇撒配套系断奶仔猪192头,随机分为4个处理,每个处理6个重复,每个重复8头,对照组饲喂基础饲粮,LF1、LF2和LF3 3个试验组在基础饲粮基础上分别添加125、250和500 mg/kg的LF。饲喂42 d后,测定平均日增重(ADG)、平均日采食量(ADFI)、料重比(F/G)和腹泻率。采用试剂盒检测血清总抗氧化能力(T-AOC)、总超氧化物歧化酶(T-SOD)活力、丙二醛(MDA)含量和谷胱甘肽过氧化物酶(GSH-Px)活力,采用实时荧光定量PCR(RT-qPCR)法测定心脏和肝脏谷氧还蛋白1(GRX1)、硫氧还蛋白1(TRX1)、超氧化物歧化酶(SOD)及GSH-Px基因表达量。结果表明:1)与对照组相比,LF2和LF3组显著提高了ADG、ADFI(P < 0.05),LF1和LF2组显著降低了料重比(P < 0.05),LF2和LF3组显著降低了仔猪的腹泻率(P < 0.05)。2)血清中,与对照组相比,LF1(P < 0.05)、LF2(P < 0.01)和LF3组(P < 0.05)T-SOD活力均显著或极显著提高;LF2组GSH-Px活力及T-AOC均显著提高(P < 0.05);LF2和LF3组的MDA含量均显著降低(P < 0.05)。3)与对照组相比,LF1和LF2组GRX1、SOD和GSH-Px基因在心脏和肝脏中的表达量均增加,其中LF1组肝脏GSH-Px差异显著(P < 0.05),LF2组肝脏GRX1、SOD和GSH-Px差异极显著(P < 0.01);LF1组TRX1基因在肝脏中的表达量极显著提高(P < 0.01);LF3组GRX1、TRX1基因在肝脏和心脏中及SOD、GSH-Px基因在心脏中的表达量均降低,其中心脏GRX1、TRX1和SOD差异极显著(P < 0.01)。结果提示:在断奶仔猪饲粮中添加一定量的LF(本试验添加250 mg/kg最佳)可提高断奶仔猪的生长性能,提高血清抗氧化指标及心脏和肝脏中抗氧化基因GRX1、TRX1、SOD和GSH-Px的表达量。

关键词： 滇撒配套系断奶仔猪; 乳铁蛋白; 生长性能; 血清抗氧化指标; 抗氧化基因

本文引用格式

安清聪 , 李岑曦 , 张春勇 , 潘洪彬 , 李美荃 , 徐娜娜 , 陈克嶙 , 郭荣富 . 乳铁蛋白对滇撒配套系断奶仔猪生产性能、血清抗氧化指标及组织抗氧化基因表达的影响[J]. 动物营养学报, 2015 , 27(7) : 2018 -2026 . DOI: 10.3969/j.issn.1006-267x.2015.07.006

Abstract

This experiment was conducted to investigate the effects of lactofferin (LF) on growth performance, serum antioxidant indices and tissue antioxidant gene expressions in Diansa weaned piglets. A total of 192 Diansa weaned piglets at the age of (28±2) days were randomly divided into 4 groups with 6 replicates in every group and with 8 piglets in every replicate. The control group was fed a basal diet, and LF1, LF2 and LF3 groups were respectively supplemented with 125, 250 and 500 mg/kg LF in the basal diet. Average daily weight (ADG), average daily feed intake (ADFI), feed to gain ratio (F/G) and diarrhea rate were determined after feeding 42 days. Kits were used for detecting serum antioxidant indices: total antioxidant capacity (T-AOC), total superoxide dismutase (T-SOD) activity, malondialdehyde (MDA) content and glutathione peroxidase (GSH-Px) activity. The expression levels of glutraredoxin 1 (GRX1), thioredoxin 1 (TRX1), superoxide dismutase (SOD) and GSH-Px genes in heart and liver were detected by real-time quantitative PCR (RT-qPCR). The results showed as follows: 1) compared with control group, LF2 and LF3 groups significantly improved ADG and ADFI (P < 0.05), LF1 and LF2 groups significantly (P < 0.05) reduced F/G, and LF2 and LF3 groups significantly reduced dairrhea rate (P < 0.05). 2) In serum, compared with control group, T-SOD activity were significantly increased in LF1 (P < 0.05), LF2 (P < 0.01) and LF3 (P < 0.05) groups, GSH-Px activity and T-AOC in LF2 group were significantly increased (P < 0.05), and the contents of MDA in LF2 and LF3 groups were significantly decreased (P < 0.05). 3) Compared with control group, the expression levels of GRX1, SOD and GSH-Px genes in heart and liver were increased in LF1 and LF2 groups. In liver, the difference of GSH-Px gene in LF1 group was significant (P < 0.05), and those of GRX1, SOD and GSH-Px genes in LF2 group were extremely significant (P < 0.01). TRX1 gene expression level in LF1 group was extremely significantly increased (P < 0.01). The expression levels of GRX1 and TRX1 genes in both tissues, SOD and GSH-Px genes in heart in LF3 group were decreased. and the differences of GRX1, TRX1 and SOD genes in heart was extremely significant (P < 0.01). The results suggest that the additon of LF (250 mg/kg was the best in this study) with a certain amount in diets can improve growth performance of weaned piglets, and enhance antioxidant capacity of piglets by improving the serum antioxidant indices and up-regulating antioxidant gene expressions.

Key words： Diansa weaned piglet; lactofferim; growth performance; serum antioxidant indice; antioxidant gene

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