动物营养学报 >

2015 , Vol. 27 >Issue 7: 2060 - 2069

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.07.011

禽营养 Poultry Nutrition

持续偏热环境对肉鸡生长性能、糖脂代谢及解偶联蛋白mRNA表达的影响

  • 甄龙 ,
  • 石玉祥 ,
  • 张敏红 ,
  • 冯京海 ,
  • 张少帅 ,
  • 彭骞骞
展开
  • 1. 河北工程大学农学院, 邯郸 056021;
    2. 中国农业科学院北京畜牧兽医研究所, 动物营养学国家重点实验室, 北京 100193

收稿日期: 2015-02-16

  网络出版日期: 2015-07-07

基金资助

国家科技支撑计划课题"畜禽健康养殖环境控制关键技术研究与集成"(2012BAD39B02);中国农业科学院科技创新团队项目(ASTIP-IAS07)

收起

Effects of Constant Moderate Temperatures on Performance, Glucose and Lipid Metabolism, Expression of Uncoupling Protein of broilers

  • ZHEN Long ,
  • SHI Yuxiang ,
  • ZHANG Minhong ,
  • FENG Jinghai ,
  • ZHANG Shaoshuai ,
  • PENG Qianqian
Expand
  • 1. College of Agriculture HeBei University of Engineering, Handan 056021, China;
    2. State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received date: 2015-02-16

  Online published: 2015-07-07

Fold

摘要

本试验研究了持续不同温度处理(21、26和31 ℃)对肉鸡生长性能、血清糖脂代谢相关指标、胸肌和肝脏解偶联蛋白(avUCP)mRNA表达的影响。试验选取22日龄爱拔益加(AA)肉鸡144只转入环境控制舱,随机分成3组,每组6个重复,每个重复8只鸡(公母各4只)。适应期7 d,温度21 ℃,相对湿度60%。29日龄时,试验温度分别调整到21、26和31 ℃,相对湿度60%,直至试验结束,共14 d。结果表明:1)31 ℃组肉鸡平均日增重(ADG)、平均日采食量(ADFI)极显著低于21、26 ℃组(P<0.01),料重比(F/G)显著高于21、26 ℃组(P<0.05);26 ℃组肉鸡ADG、ADFI显著低于21 ℃组(P<0.05),F/G和21 ℃组无显著差异(P>0.05)。2)31 ℃组肉鸡血清葡萄糖(GLU)、总胆固醇(TC)、甘油三酯(TG)、游离脂肪酸(FFA)含量显著高于21 ℃组(P<0.05);26 ℃组血清生化指标与21 ℃组无显著差异(P>0.05)。3)31 ℃组肉鸡血清甲状腺素(T4)、瘦素(LEP)、皮质酮(CORT)含量显著高于21 ℃组(P<0.05);除T4含量外(P<0.05),26 ℃与21 ℃组之间血清激素指标无显著差异(P>0.05)。4)试验第7天,31 ℃组胸肌avUCP mRNA表达显著低于21、26 ℃组(P<0.05);第14天,26、31 ℃组胸肌avUCP mRNA表达极显著低于21 ℃组(P<0.01);试验第7、14天,31 ℃组肉鸡肝脏avUCP mRNA表达极显著高于21、26 ℃组(P<0.01)。综上,与21 ℃相比,持续偏热处理(26、31 ℃)影响肉鸡糖脂代谢及avUCP mRNA的表达,并显著降低生长性能,且不同偏热程度对肉鸡影响程度不同。

关键词： 偏热环境; 肉鸡; 生长性能; 糖脂代谢; 解偶联蛋白

本文引用格式

甄龙 , 石玉祥 , 张敏红 , 冯京海 , 张少帅 , 彭骞骞 . 持续偏热环境对肉鸡生长性能、糖脂代谢及解偶联蛋白mRNA表达的影响[J]. 动物营养学报, 2015 , 27(7) : 2060 -2069 . DOI: 10.3969/j.issn.1006-267x.2015.07.011

Abstract

This experiment was conducted to study the effects of three different constant ambient temperatures (21, 26 and 31 ℃) on performance, serum biochemical and hormone indices related in glucose and lipid metabolism, and expression of uncoupling protein in pectoralis muscle and liver of broilers. One hundred and forty-four 22-day-old Arbor Acres (AA) broilers were assigned to three environment chambers, each chamber contained six cages with eight birds per cage(with four males and four females), and each cage as a replicate. The pre-test period lasted for 7 days and broilers were kept at 21 ℃ and 60% relative humidity. When broilers were 29-day-old, the temperatures of each environmental chamber were gradually regulated to 21, 26 and 31 ℃, respectively, while maintaining the relative humidity at 60% and both kept constant until the end of the experiment. The trial period lasted for 14 days. The results showed as follows: 1) average daily gain (ADG), average daily food intake (ADFI) and food to gain ratio (F/G) in 31 ℃ group showed highly significant (P < 0.01) depression than that in 21 and 26 ℃ groups; ADG and ADFI in 26 ℃ group were significant (P < 0.05) lower than that in 21 ℃ group, with no significant difference (P > 0.05) in F/G was found between the two groups. 2) serum glucose (GLU), total cholesterol (TC), total triglyceride (TG) and nonestesterified fatty acid (FFA) in 31 ℃ were significant (P < 0.05) higher than that in 21 ℃ group, with no significant difference (P > 0.05) was found between 21 and 26 ℃ groups. 3) serum thyroxine (T4), leptin (LEP) and corticosterone (CORT) in 31 ℃ were significant (P < 0.05) higher than that in 21 ℃ group, with no significant difference (P > 0.05) was found between 21 and 26 ℃ groups except for T4. 4) expression of avian uncoupling protein (avUCP) mRNA in pectoral muscle of broilers in 31 ℃ group showed highly significant (P < 0.01) depression than that in 21 and 26 ℃ groups at the seventh day, with highly significant (P < 0.01) depression in 26 and 31 ℃ groups than 21 ℃ at the fourteenth day; throughout the trial period, expression of avUCP mRNA in liver of broilers in 31 ℃ group was highly significant (P < 0.01) higher than that in 21 and 26 ℃ groups. In conclusion, compared with 21 ℃ group, the constant moderate heat stress (26 and 31 ℃) can the performance, glucose and lipid metabolism, and expression of uncoupling protein of broilers. And the different extent moderate heat stress would produce different effects on that.

Key words： moderate temperature; broiler; performance; glucose and lipid metabolism; avian uncoupling protein

参考文献

[1] AL-FATAFTAH A A,ABU-DIEYEH Z H M.Effect of chronic heat stress on broiler performance in Jordan[J].International Journal of Poultry Science,2007,6(1):64-70.  

[2] SOHAIL M U,HUME M E,BYRD J A,et al.Effect of supplementation of prebiotic mannan-oligosaccharides and probiotic mixture on growth performance of broilers subjected to chronic heat stress[J].Poultry Science,2012,91(9):2235-2240.  

[3] 唐丽.热应激对肉种母鸡繁殖性能、相关生理生化和分子指标的影响[D].硕士学位论文.北京:中国农业科学院,2013.

[4] 罗庆斌,何丹林.鸡热应激研究概述[J].养禽与禽病防治,2005(4):2-5.

[5] 刘梅.急性热应激对肉仔鸡生长性能及脂肪代谢的影响[J].动物营养学报,2011,23(5):862-868.

[6] GERAERT P A,PADILHA J C F,GUILAUMIN S.Metabolic and endocrine changes induced by chronic heat exposure in broiler chickens:growth performance,body composition and energy retention[J].British Journal of Nutrition,1996,75(2):195-204.

[7] RAIMBAULT S,DRIDI S,DENJEAN F,et al.An uncoupling protein homologue putatively involved in facultative muscle thermogenesis in birds[J].Biochemical Journal,2001,353:441-444.

[8] WANG J T,ZHANG X J,XU S W.Effects of cold stress on energy metabolism in the chicken[J].Chinese Journal of Applied Physiology,2009,25(2):172-176.

[9] ROUSSEL D,ROUANET J L,DUCHAMP C,et al.Effects of cold acclimation and palmitate on energy coupling in duckling skeletal muscle mitochondria[J].FEBS Letters,1998,439(3):258-262.  

[10] MUJAHID A,SATO K,AKIBA Y,et al.Acute heat stress stimulates mitochondrial superoxide production in broiler skeletal muscle,possibly via downregulation of uncoupling protein content[J].Poultry Science,2006,85(7):1259-1265.  

[11] TAOUIS M,DE BASILIO V,MIGNON-GRASTEAU S,et al.Early-age thermal conditioning reduces uncoupling protein messenger RNA expression in pectoral muscle of broiler chicks at seven days of age[J].Poultry Science,2002,81(11):1640-1643.  

[12] 张敏红,苏红光,冯京海,等.采集用于建立肉鸡生活环境舒适性评价模型数据的方法和专用装置:中国,CN103404447A[P/OL].2013-11-27.

[13] HARRIS G C,Jr,DODGEN W H,NELSON G S.Effects of diurnal cyclic growing temperatures on broiler performance[J].Poultry Science,1974,53(6):2204-2208.  

[14] DONKOH A.Ambient temperature:a factor affecting performance and physiological response of broiler chickens [J].International Journal of Biometeorology,1989,33(4):259-265.  

[15] 苏红光,张敏红,冯京海,等.持续冷热环境对肉鸡生长性能、糖代谢和解偶联蛋白mRNA表达的影响[J].动物营养学报,2014,26(11):3276-3283.

[16] 甄龙,张少帅,石玉祥,等.水料比作为偏热环境肉鸡热舒适评价指标研究[J].动物营养学报,2015,27(6):1750-1758.

[17] 王玢,左明雪.人体及动物生理学[M].2版.北京:高等教育出版社,2001.

[18] 王启军.高温环境对不同生长阶段北京油鸡脂肪沉积及脂质代谢的影响[D].硕士学位论文.杨凌:西北农林科技大学,2006.

[19] VECEREK V.Influence of high environmental temperature on production and haematological and biochemical indexes in broiler chickens[J].Czech Journal of Animal Science,2002,47(5):176-182.

[20] 周杰,骆先虎,檀其梅,等.高温对肉用仔鸡生产性能和某些血清生化指标的影响[J].畜牧与兽医,1997,29(2):57-59.

[21] 李军桥.高温环境对肉鸡血液生化指标、热应激蛋白(HSP72)转录及肉品质的影响[D].硕士学位论文.保定:河北农业大学,2004.

[22] KOUBA M,HERMIER D,LE DIVIDICH J.Influence of a high ambient temperature on lipid metabolism[J].Journal of Animal Science,2001,79(1):81-87.

[23] 卢庆萍.高温环境下不同基因型肉鸡肉质性状及脂肪沉积规律的研究[D].博士学位论文.北京:中国农业科学院,2007.

[24] TAO X,ZHANG Z Y,DONG H,et al.Responses of thyroid hormones of market-size broilers to thermoneutral constant and warm cyclic temperatures[J].Poultry Science,2006,85(9):1520-1528.  

[25] 顾宪红,王新谋.高温对蛋鸡生产性能和血浆皮质酮,甲状腺素,孕酮水平的影响[J].畜牧兽医学报,1995,26(2):109-1115.

[26] 刘思当,宁章勇,谭勋.热应激对肉仔鸡血液生化指标影响的观察[J].中国兽医杂志,2003,39(9):20-23.

[27] 刘凤华,吴国娟,王占贺,等.热应激中仔鸡血液生化指标及T细胞变化规律[J].中国兽医杂志,2004,40(6):11-13.

[28] 杜荣,顾宪红.环境温度和日粮能量水平对鸡血浆皮质酮水平的影响[J].畜牧兽医学报,1997,28(2):126-129.

[29] DEYHIM F,TEETER R G.Research note:sodium and potassium chloride drinking water supplementation effects on acid-base balance and plasma corticosterone in broilers reared in thermoneutral and heat-distressed environments[J].Poultry Science,1991,70(12):2551-2553.  

[30] GERAERT P A,PADILHA J C F,GUILLAUMIN S.Metabolic and endocrine changes induced by chronic heat exposure in broiler chickens:biological and endocrinological variables[J].British Journal of Nutrition,1996,75(2):205-216.

[31] BREUNER C W,SPRAGUE R S,PATTERSON S H,et al.Environment,behavior and physiology:do birds use barometric pressure to predict storms?[J].The Journal of Experimental Biology,2013,216(Pt11):1982-1990.

[32] 郝靖宇.持续高温对不同性别北京油鸡脂肪沉积和脂肪代谢的影响[D].硕士学位论文.北京:中国农业科学院,2011.

[33] TOYOMIZU M,UEDA M,SATO S,et al.Cold-induced mitochondrial uncoupling and expression of chicken UCP and ANT mRNA in chicken skeletal muscle[J].FEBS Letters,2002,529(2/3):313-318.

[34] GASPARINO E,DEL VESCO A P,VOLTOLINI D M,et al.The effect of heat stress on GHR,IGF-Ⅰ,ANT,UCP and COXⅢ mRNA expression in the liver and muscle of high and low feed efficiency female quail[J].British Poultry Science,2014,55(4):466-473.  

[35] EVOCK-CLOVER C M,POCH A M,RICHARDS M P,et al.Expression of an uncoupling protein gene homolog in chickens[J].Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology,2002,133(2):345-358.  

[36] DRIDI S,ONAGBESAN O,SWENNEN Q,et al.Gene expression,tissue distribution and potential physiological role of uncoupling protein in avian specees[J].Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology,2004,139(3):273-283.  

[37] LARKIN S,MULL E,MIAO W,et al.Regulation of the third member of the uncoupling protein family,UCP3,by cold and thyroid hormone[J].Biochemical and Biophysical Research Communications,1997,240(1):222-227.  
Options
文章导航

/

〈 〉

    联系我们

  • 地址：北京市海淀区圆明园西路2号
    　　　中国农业大学动科动医大楼153室
  • 邮编：100193
  • 电话：010－62817823
  • 网址：http://www.chinajan.com
  • E-mail：cjan@chinajan.com
京公网安备 11010802041926号    京ICP备17050989号-2