动物营养学报 >

2015 , Vol. 27 >Issue 7: 2300 - 2308

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.07.038

研究简报 Short Communications

饲粮脂肪水平对冬毛期银狐生长性能、体脂肪酸组成及空肠中小肠型脂肪酸结合蛋白表达的影响

  • 张婷 ,
  • 钟伟 ,
  • 罗婧 ,
  • 周宁 ,
  • 黄健 ,
  • 王卓 ,
  • 孙皓然 ,
  • 樊燕燕 ,
  • 李光玉
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  • 中国农业科学院特产研究所, 吉林省特种经济动物分子生物学省部共建实验室, 长春 130112

收稿日期: 2015-01-19

  网络出版日期: 2015-07-07

基金资助

吉林省自然科学基金项目(20140101033JC);吉林省特种经济动物营养与生理研究创新团队项目(20121810)

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Effects of Dietary Fat Level on Growth Performance, Body Fatty acid Composition and the Expression of Intestinal Fatty Acid Binding Protein in Jejunum of Silver Foxes during Winter Fur-Growing Period

  • ZHANG Ting ,
  • ZHONG Wei ,
  • LUO Jing ,
  • ZHOU Ning ,
  • HUANG Jian ,
  • WANG Zhuo ,
  • SUN Haoran ,
  • FAN Yanyan ,
  • LI Guangyu
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  • State Key Laboratory of Special Economic Animal Molecular Biology, Institute of Special Animal and Plant Science, Chinese Academy of Agricultural Science, Changchun 130112, China

Received date: 2015-01-19

  Online published: 2015-07-07

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摘要

为研究饲粮脂肪水平对冬毛期银狐生长性能、体脂肪酸组成及空肠中小肠脂肪酸结合蛋白(I-FABP)表达的影响,选取145日龄、体重相近的健康雄性银狐50只,随机分成5组(每组10个重复,每个重复1只),分别饲喂含10%、12%、16%、20%、24%混合油脂(鸡油:豆油=1:1)的试验饲粮,试验饲粮实测脂肪水平分别为12.85%(A组)、14.71%(B组)、18.72%(C组)、22.23%(D组)、26.11%(E组)。预试期为15 d,正试期为75 d。结果显示:1)饲粮脂肪水平显著影响冬毛期银狐的平均日采食量(ADFI)(P<0.01),ADFI随着饲粮脂肪水平的升高而降低;B、C、D组的平均日增重(ADG)极显著高于A、E组(P<0.01);D组料重比(F/G)显著低于A、B、E组(P<0.05)。2)随着饲粮脂肪水平的升高,肌间脂肪中饱和脂肪酸(SFA)的比例升高,单不饱和脂肪酸(MUFA)的比例下降(P<0.05),而多不饱和脂肪酸(PUFA)的比例无显著变化(P>0.05)。3)饲粮脂肪水平对银狐皮下腹部脂肪中SFA、MUFA和PUFA的比例无显著影响(P>0.05)。4)随饲粮脂肪水平的升高,空肠中I-FABP mRNA的相对表达量呈上升趋势,表现为E组显著高于A、B、C组(P<0.05)。由此得出,当饲粮脂肪水平达到18.72%时,冬毛期银狐表现出最佳的生长性能;银狐体脂肪酸组成能够反映饲粮的脂肪酸组成,肌间脂肪脂肪酸组成受饲粮脂肪水平的影响,而皮下脂肪脂肪酸组成相对稳定;此外,提高饲粮脂肪水平可促进冬毛期银狐空肠中I-FABP的表达。

关键词： 银狐; 脂肪水平; 生长性能; 脂肪酸组成; 小肠型脂肪酸结合蛋白

本文引用格式

张婷 , 钟伟 , 罗婧 , 周宁 , 黄健 , 王卓 , 孙皓然 , 樊燕燕 , 李光玉 . 饲粮脂肪水平对冬毛期银狐生长性能、体脂肪酸组成及空肠中小肠型脂肪酸结合蛋白表达的影响[J]. 动物营养学报, 2015 , 27(7) : 2300 -2308 . DOI: 10.3969/j.issn.1006-267x.2015.07.038

Abstract

This experiment was conducted to study the effects of dietary fat level on growth performance, body fatty acid composition and the expression of intestinal fatty acid binding protein (I-FABP) in jejunum of silver foxes during winter fur-growing period. Fifty 145-day-old male silver foxes with a similar body weight were randomly divided into 5 groups with 10 replicates per group and 1 fox per replicate. Foxes in those 5 groups were fed the experimental diets contained 10%, 12%, 16%, 20%, and 24% lipid mixture (soybean oil:chicken oil=1:1), and measured value of total fat level of those diets was 12.85% (group A), 14.71% (group B), 18.72% (group C), 22.23% (group D) and 26.11% (group E), respectively. The pre-test period lasted for 15 days and the trial period lasted for 75 days. The results showed as follows: 1) the average daily feed intake (ADFI) was significantly affected by dietary fat level (P<0.01), and the ADFI showed a decrease trend with the dietary fat level increasing; the average daily gain (ADG) in groups B, C and D was significantly higher than that in groups A and E (P<0.01); the feed/gain (F/G) in group D was significantly lower than that in groups A, B and E (P<0.05). 2) With the dietary fat level increasing, the proportion of saturated fatty acids (SFA) of intermuscular fat was increased, and the proportion of monounsaturated fatty acids (MUFA) was decreased, while the proportion of polyunsaturated fatty acids (PUFA) had no significant change (P>0.05). 3) Dietary fat level had no significant effects on the proportion of SFA, MUFA and PUFA of subcutaneous fat of silver foxes (P>0.05). 4) The relative expression of I-FABP mRNA in jejunum was increased with the dietary fat level increasing, and it showed that group E was significantly higher than groups A, B and C (P<0.05). In conclusion, the silver foxes have the best growth performance when fed diets with 18.72% fat during winter fur-growing period; body fatty acid composition of silver foxes reflect the dietary fatty acid composition, the fatty acid composition of intermuscular fat is affected by dietary fat level, while the fatty acid composition of subcutaneous fat is stable; moreover, increasing dietary fat level can improve the expression of I-FABP in jejunum of silver foxes during winter fur-growing period.

Key words： silver foxes; fat level; growth performance; fatty acid composition; intestinal fatty acid binding protein

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