综述 Review

反刍动物瘤胃高效产氨菌菌群结构、功能及其调控

  • 申军士 ,
  • 毛胜勇 ,
  • 朱伟云
展开
  • 江苏省消化道营养与动物健康重点实验室, 南京农业大学消化道微生物研究室, 南京 210095

收稿日期: 2015-03-02

  网络出版日期: 2015-08-13

基金资助

国家自然科学基金青年基金(31402101);江苏省自然科学基金青年基金(BK20140696)

Ruminal Hyper Ammonia Producing Bacteria in Ruminants: Community Structure, Function and Its Manipulation

  • SHEN Junshi ,
  • MAO Shengyong ,
  • ZHU Weiyun
Expand
  • Laboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China

Received date: 2015-03-02

  Online published: 2015-08-13

摘要

对于反刍动物来说,氮的平均利用效率仅为25%左右,绝大部分无效氮随粪尿排出体外。氮素的大量流失不仅造成饲料蛋白质资源浪费,而且加剧环境污染。而反刍动物瘤胃中高效产氨菌(HAB)产氨速率过快,是导致反刍动物氮利用率低的主要原因之一。影响瘤胃内HAB菌群结构与功能的因素有很多,离子载体、植物提取物、细菌分泌的细菌素、饲粮类型等因素均可对瘤胃HAB菌群结构与功能产生影响。本文对瘤胃HAB菌群结构、功能及其调控手段进行了综述,以期为提高反刍动物氮利用效率提供科学依据。

本文引用格式

申军士 , 毛胜勇 , 朱伟云 . 反刍动物瘤胃高效产氨菌菌群结构、功能及其调控[J]. 动物营养学报, 2015 , 27(8) : 2323 -2327 . DOI: 10.3969/j.issn.1006-267x.2015.08.002

Abstract

The average nitrogen utilization efficiency in ruminants is only around 25%, and most of inefficient nitrogen is excreted via manure. Excess nitrogen loss is not only a waste of feed protein resources, but also leads to serious environmental pollution. Rapid amino acids deamination by ruminal hyper ammonia-producing bacteria (HAB) is one of the main causes for the inefficient use of nitrogen. The community structure and function of ruminal HAB could be affected by many factors, such as ionophore, plant extracts, bacteriocin, and diet types. This review summarizes community structure and function of ruminal HAB in ruminants and its manipulation methods, which aims to provide scientific basis for the improvement of nitrogen utilization efficiency in ruminants.

参考文献

[1] CALSAMIGLIA S,FERRET A,REYNOLDS C K,et al.Strategies for optimizing nitrogen use by ruminants[J].Animal,2010,4(7):1184-1196.  

[2] TAMMINGA S.Nutrition management of dairy cows as a contribution to pollution control[J].Journal of Dairy Science,1992,75(1):345-357.  

[3] RYCHLIK J L,RUSSELL J B.Mathematical estimations of hyper-ammonia producing ruminal bacteria and evidence for bacterial antagonism that decreases ruminal ammonia production[J].FEMS Microbiology Ecology,2000,32(2):121-128.

[4] LOBLEY G E,BREMNER D M,ZUUR G.Effects of diet quality on urea fates in sheep as assessed by refined,non-invasive[15N15N] urea kinetics[J].The British Journal of Nutrition,2000,84(4):459-468.

[5] CHEN G,RUSSELL J B.More monensin-sensitive,ammonia-producing bacteria from the rumen[J].Applied and Environmental Microbiology,1989,55(5):1052-1057.

[6] BACH A,CALSAMIGLIA S,STERN M D.Nitrogen metabolism in the rumen[J].Journal of Dairy Science,2005,88(Suppl.1):E9-E21.  

[7] WALLACE R J.Ruminal microbial metabolism of peptides and amino acids[J].The Journal of Nutrition,1996,126(4Suppl.):1326S-1334S.

[8] RUSSELL J B,STROBEL H J,CHEN G J.Enrichment and isolation of a ruminal bacterium with a very high specific activity of ammonia production[J].Applied and Environmental Microbiology,1988,54(4):872-877.

[9] PASTER B J,RUSSELL J B,YANG C M J,et al.Phylogeny of the ammonia-producing ruminal bacteria Peptostreptococcus anaerobius,Clostridium sticklandii,and Clostridium aminophilum sp. nov[J].International Journal of Systematic Bacteriology,1993,43(1):107-110.  

[10] ATTWOOD G T,KLIEVE A V,OUWERKERK D,et al.Ammonia-hyperproducing bacteria from New Zealand ruminants[J].Applied and Environmental Microbiology,1998,64(5):1796-1804.

[11] MCSWEENEY C S,PALMER B,BUNCH R,et al.Isolation and characterization of proteolytic ruminal bacteria from sheep and goats fed the tannin-containing shrub legume Calliandra calothyrsus[J].Applied and Environmental Microbiology,1999,65(7):3075-3083.

[12] FLYTHE M D,ANDRIES K.The effects of monensin on amino acid catabolizing bacteria isolated from the Boer goat rumen[J].Small Ruminant Research,2009,81(2/3):178-181.

[13] ESCHENLAUER S C P,MCKAIN N,WALKER N D,et al.Ammonia production by ruminal microorganisms and enumeration,isolation,and characterization of bacteria capable of growth on peptides and amino acids from the sheep rumen[J].Applied and Environmental Microbiology,2002,68(10):4925-4931.  

[14] WALLACE R J,MCKAIN N,MCEWAN N R,et al.Eubacterium pyruvativorans sp. nov.,a novel non-saccharolytic anaerobe from the rumen that ferments pyruvate and amino acids,forms caproate and utilizes acetate and propionate[J].International Journal of Systematic and Evolutionary Microbiology,2003,53(Pt 4):965-970.

[15] RICHARDSON A J,MCKAIN N,WALLACE R J.Ammonia production by human faecal bacteria,and the enumeration,isolation and characterization of bacteria capable of growth on peptides and amino acids[J].BMC Microbiology,2013,13:6.  

[16] 王志博,辛杭书,段春宇,等.饲粮中添加海南霉素和莫能菌素对奶牛瘤胃蛋白质降解和产氨菌群的影响[J].中国农业科学,2012,45(14):2959-2966.

[17] KRAUSE D O,RUSSELL J B.An rRNA approach for assessing the role of obligate amino acid-fermenting bacteria in ruminal amino acid deamination[J].Applied and Environmental Microbiology,1996,62(3):815-821.

[18] WANG Z B,XIN H S,WANG M J,et al.Effects of dietary supplementation with hainanmycin on protein degradation and populations of ammonia-producing bacteria in vitro[J].Asian-Australasian Journal of Animal Sciences,2013,26(5):668-674.  

[19] STIVERSON J,MORRISON M,YU Z T.Populations of select cultured and uncultured bacteria in the rumen of sheep and the effect of diets and ruminal fractions[J].International Journal of Microbiology,2011,2011:Article ID 750613.

[20] PATRA A K,YU Z T.Effects of vanillin,quillaja saponin,and essential oils on in vitro fermentation and protein-degrading microorganisms of the rumen[J].Applied Microbiology and Biotechnology,2014,98(2):897-905.  

[21] CHEN G J,RUSSELL J B.Effect of monensin and a protonophore on protein degradation,peptide accumulation,and deamination by mixed ruminal microorganisms in vitro[J].Journal of Animal Science,1991,69(5):2196-2203.

[22] YANG C M,RUSSELL J B.The effect of monensin supplementation on ruminal ammonia accumulation in vivo and the numbers of amino acid-fermenting bacteria[J].Journal of Animal Science,1993,71(12):3470-3476.

[23] RYCHLIK J L,RUSSELL J B.The adaptation and resistance of Clostridium aminophilum F to the butyrivibriocin-like substance of Butyrivibrio fibrisolvens JL5 and monensin[J].FEMS Microbiology Letters,2002,209(1):93-98.  

[24] KLEVENHUSEN F,MURO-REYES A,KHIAOSA-ARD R,et al.A meta-analysis of effects of chemical composition of incubated diet and bioactive compounds on in vitro ruminal fermentation[J].Animal Feed Science and Technology,2012,176(1/2/3/4):61-59.  

[25] FLYTHE M D.The antimicrobial effects of hops (Humulus lupulus L.) on ruminal hyper ammonia-producing bacteria[J].Letters in Applied Microbiology,2009,48(6):712-717.

[26] MCINTOSH F M,WILLIAMS P,LOSA R,et al.Effects of essential oils on ruminal microorganisms and their protein metabolism[J].Applied and Environmental Microbiology,2003,69(8):5011-5014.  

[27] FLYTHE M,KAGAN I.Antimicrobial effect of red clover (Trifolium pratense) phenolic extract on the ruminal hyper ammonia-producing bacterium,Clostridium sticklandii[J].Current Microbiology,2010,61(2):125-131.  

[28] RAVIKUMAR K,SUNEETHA V,THANISLASS J.Ruminal bacteriocins—an effective tool in preventing lactic acidosis in ruminants[J].Research Journal of Science and Technology,2015,6(4):220-224.

[29] CALLAWAY T R,DE MELO A M S C,RUSSELL J B.The effect of nisin and monensin on ruminal fermentations in vitro[J].Current Microbiology,1997,35(2):90-96.  

[30] RYCHLIK J L,RUSSELL J B.Bacteriocin-like activity of Butyrivibrio fibrisolvens JL5 and its effect on other ruminal bacteria and ammonia production[J].Applied and Environmental Microbiology,2002,68(3):1040-1046.  

[31] LIMA J R,RIBON A O,RUSSELL J B,et al.Bovicin HC5 inhibits wasteful amino acid degradation by mixed ruminal bacteria in vitro[J].FEMS Microbiology Letters,2009,292(1):78-84.  
文章导航

/