烟酸对水牛瘤胃发酵指标及微生物区系的影响

林波; 梁辛; 韦升菊; 李丽莉; 梁贤威; 李萍; 邹彩霞

doi:10.3969/j.issn.1006-267x.2015.08.011

动物营养学报 >

2015 , Vol. 27 >Issue 8: 2396 - 2404

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.08.011

反刍动物营养 Ruminant Nutrition

烟酸对水牛瘤胃发酵指标及微生物区系的影响

林波 ,
梁辛 ,
韦升菊 ,
李丽莉 ,
梁贤威 ,
李萍 ,
邹彩霞

展开

中国农业科学院广西水牛研究所, 农业部(广西)水牛遗传繁育重点实验室, 南宁 530001

收稿日期: 2015-03-02

网络出版日期: 2015-08-13

基金资助

国家自然科学基金项目(31160470);广西自然科学基金项目(2013GXNSFBA019114)

收起

Effects of Niacin on Ruminal Fermentation Indexes and Microflora of Water Buffaloes

LIN Bo ,
LIANG Xin ,
WEI Shengju ,
LI Lili ,
LIANG Xianwei ,
LI Ping ,
ZOU Caixia

Expand

Key Laboratory of Buffalo Genetics, Breeding and Reproduction, Ministry of Agriculture and Guangxi, Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning 530001, China

Received date: 2015-03-02

Online published: 2015-08-13

Fold

摘要

本试验旨在研究饲粮添加烟酸对水牛瘤胃发酵指标及微生物区系的影响。试验选用 36头健康状况良好,体重接近的杂交泌乳水牛,随机分为4个组,分别在基础饲粮中添加0(对照)、4、8、12 g/d烟酸,烟酸与精料混合饲喂,进行预试期14 d,正试期42 d的饲养试验。试验结束后,胃管采集瘤胃液测定瘤胃发酵指标,并采用Illumina Miseq PE250高通量测序平台分别测序瘤胃细菌和甲烷菌16S rRNA基因以确定二者区系组成。结果表明:1)与对照组相比,添加12 g/d烟酸提高了水牛日均采食量(P<0.05)和瘤胃液氨态氮浓度(P>0.05),烟酸各组均显著降低了瘤胃液总挥发性脂肪(TVFA)浓度(P<0.05),但未显著影响瘤胃液pH(P>0.05)。2)水牛瘤胃内细菌在门水平上主要以拟杆菌门和厚壁菌门为主,科水平上为普雷沃氏菌科和黄杆菌科;甲烷菌以甲烷短杆菌属为主,占90%以上,其次为甲烷球菌属和热原体属,水牛瘤胃微生物区系总体上与奶牛等其他利用植物纤维的反刍动物相似。3)与对照组相比,饲粮添加烟酸显著降低了瘤胃内厚壁菌门的比例(P<0.05),显著提高了黄杆菌科细菌的比例(P<0.05),但对瘤胃甲烷菌区系无显著影响(P>0.05)。饲粮添加烟酸改变了水牛瘤胃发酵指标和微生物区系组成,瘤胃发酵指标的变化除了与烟酸对水牛热应激的缓解和消化吸收的影响有关,还与烟酸添加对瘤胃细菌区系的影响有关。

关键词： 烟酸; 水牛; 瘤胃发酵; 微生物区系

本文引用格式

林波 , 梁辛 , 韦升菊 , 李丽莉 , 梁贤威 , 李萍 , 邹彩霞 . 烟酸对水牛瘤胃发酵指标及微生物区系的影响[J]. 动物营养学报, 2015 , 27(8) : 2396 -2404 . DOI: 10.3969/j.issn.1006-267x.2015.08.011

Abstract

This study was conducted to study the effects of dietary addition of niacin on ruminal fermentation indexes and microflora of water buffaloes. Thirty-six healthy hybrid water buffaloes with similar body weight were selected and divided into four groups randomly. The four groups were fed the same basal diet but added with 0 (control), 4, 8 and 12 g/d, respectively. Niacin was mixed in concentrate to feed. The advanced experiment feeding period was 14 days and the formal experimental period was 42 days. At the end of experiment, rumen fluid samples were taken from rumen by stomach tube to test the ruminal fermentation indexes, 16S rRNA genes of ruminal bacteria and methanogen were tested to determine microbial community by Illumina Miseq PE250 high-throughput sequencing. The results showed as follows: 1) compared with control group, the addition of 12 g/d of niacin increased average daily feed intake (P<0.05) and rumen fluid ammonia concentration (P>0.05), niacin groups significantly decreased rumen fluid total volatile fatty acid (TVFA) concentration (P<0.05), but the rumen fluid pH was not significantly influenced (P>0.05). 2) Water buffalo ruminal bacterial community was dominated by Bacteroidetes and Firmicutes at phyla level, Prevotellaceae and Fibrobacteraceae at family level, while ruminal methanogen community was dominated by Methanobrevibacter (over 90%) at genus level, followed by Methanosphaera and Thermoplasma. Water buffalo ruminal bacteria and methanogen community composition was generally similar with other ruminants which could utilize plant fiber. 3) Compared with control group, the addition of niacin significantly decreased ruminal Firmicutes the proportion (P<0.05), and significantly increased ruminal Fibrobacteraceae proportion (P<0.05), but there was no influence on methanogen community (P>0.05). In conclusion, the addition of niacin changes ruminal fermentation indexes and microflora of water buffaloes, the changes of ruminal fermentation indexes on one hand are related with release on heat stress and improvement on digestion of niacin, on another hand are related with the change of ruminal microflora.

Key words： niacin; water buffalo; ruminal fermentation; microflora

参考文献

[1] 章纯熙.中国水牛科学[M].南宁:广西科学技术出版社,2010.

[2] 蒋亚军,周凌云,赵芸君,等. 烟酸在反刍动物营养中的研究进展[J].中国畜牧兽医,2010,37(7):9-14.

[3] 李萍,梁辛,韦升菊,等.不同水平烟酸对夏季泌乳水牛饲粮养分消化率和生产性能的影响[J].动物营养报,2014,26(9):2630-2636.

[4] 王菊花,卢德勋,冯宗慈,等.添加烟酸条件下绵羊瘤胃发酵底物降解动力学变化特征[J].中国草食动物,2008,28(6):7-10.

[5] DOREAU M,OTTOU J F.Influence of niacin supplementation on in vivo digestibility and ruminal digestion in dairy cows[J].Journal of Dairy Science,1996,79(12):2247-2254.

[6] 杨艳,瞿明仁,欧阳克蕙,等.烟酸对锦江黄牛瘤胃乳酸、挥发性脂肪酸浓度及相关酶活性的影响[J].动物营养学报,2013,25(7):1610-1616.

[7] SAMANTA A K,KEWALRAMANI N,KAUR H.Effect of niacin supplementation on VFA production and microbial protein synthesis in cattle[J].Journal of Dairy Science,2000,53(2):150-153.

[8] 张丽英.饲料分析及饲料质量检测技术[M].3版.北京:中国农业大学出版社,2007.

[9] 冯宗慈,高民.通过比色测定瘤胃液氨氮含量方法的改进[J].内蒙古畜牧科学,1993(4):40-41.

[10] 胡伟莲,王佳堃,吕建敏,等.瘤胃体外发酵产物中的甲烷和有机酸含量的快速测定[J].浙江大学学报:农业与生命科学版,2006,32(2):217-221.

[11] KITTELMANN S,SEEDORF H,WALTERS W A,et al.Simultaneous amplicon sequencing to explore co-occurrence patterns of bacterial,archaeal and eukaryotic microorganisms in rumen microbial communities[J].PLoS One,2013,8(2):e47879.

[12] CAPORASO J G,KUCZYNSKI J,STOMBAUGH J,et al.QIIME allows analysis of high-throughput community sequencing data[J].Nature Methods,2010,7(5):335-336.

[13] OTTOU J F,DOREAU M.Influence of niacin on in vitro ruminal fermentation and microbial synthesis depending on dietary factors[J].Animal Feed Science and Technology,1996,58(3/4):187-199.

[14] 欧阳克蕙,张琪,鲁友友,等.高精料饲粮中添加烟酸对体外瘤胃发酵培养液pH及发酵参数动态变化的影响动[J].动物营养学报,2014,26(1):115-124.

[15] 潘龙,卜登攀,程建波,等.烟酸对奶牛瘤胃发酵及缓解热应激的影响[J].中国饲料,2013(7):25-27,31.

[16] TAJIMA K,NONAKA I,HIGUCHI K,et al.Influence of high temperature and humidity on rumen bacterial diversity in Holstein heifers[J].Anaerobe,2007,13(2):57-64.

[17] JAMI E,MIZRAHI I.Composition and similarity of bovine rumen microbiota across individual animals[J].PLoS ONE,2012,7(3):e33306.

[18] SINGH K M,AHIR V B,TRIPATHI A K,et al.Metagenomic analysis of Surti buffalo (Bubalus bubalis) rumen:a preliminary study[J].Molecular Biological Repotts,2012,39(4):4841-4848.

[19] LEE H J,JUNG J Y,OH Y K,et al.Comparative survey of rumen microbial communities and metabolites across one caprine and three bovine groups,using bar-coded pyrosequencing and 1H nuclear magnetic resonance spectroscopy[J].Applied and Environmental Microbiology,2012,78(17):5983-5993.

[20] KIM M,WANG L,MORRISON M,et al.Development of a phylogenetic microarray for comprehensive analysis of ruminal bacterial communities[J].Journal of Applied Microbiology,2014,117(4):949-960.

[21] 林波,纪苗苗,梁权,等.肉桂油和牛至油及其主要成分对体外瘤胃发酵和甲烷产生的影响[J].中国兽医学报,2011,31(2):279-282,287.

[22] ST-PIERRE B,WRIGHT A D G.Diversity of gut methanogens in herbivorous animals[J].Animal,2013,7(Suppl.1):49-56.

[23] CHAUDHARY P P,SIROHI S K.Dominance of Methanomicrobium phylotype in methanogen population present in Murrah buffaloes (Bubalus bubalis)[J].Letters in Applied Microbiology,2009,49(2):274-277.

[24] CHAUDHARY P P,SIROHI S K,SINGH D,et al.Methyl coenzyme M reductase (mcrA) gene based phylogenetic analysis of methanogens population in Murrah buffaloes (Bubalus bubalis)[J].The Journal of Microbiology,2011,49(4):558-561.

[25] SINGH K M,PANDYA P R,PARNERKAR S,et al.Molecular identification of methanogenic archaea from Surti buffaloes (Bubalus bubalis),reveals more hydrogenotrophic methanogens phylotypes[J].Brazil Journal of Microbiology,2011,42(1):132-139.

[26] FRANZOLIN R,ST-PIERRE B,NORTHWOOD K,et al.Analysis of rumen methanogen diversity in water buffaloes (Bubalus bubalis) under three different diets[J].Microbial Ecology,2012,64(1):131-139.

Options

文章导航

摘要

本文引用格式

Abstract

参考文献

关于本刊

在线期刊

作者中心

相关单位

联系我们

模态框（Modal）标题

摘要

本文引用格式

Abstract

参考文献

关于本刊

在线期刊

作者中心

相关单位

联系我们